Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; n = 50) or control (without GTP; n = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.     

预混合饲料的生产技术要点及使用

预混合饲料是同一类的多种添加剂或不同类型的多种添加剂按一定比例配制而成的匀质混合物。虽然预混合饲料在全价饲料中所占比例很小,但其对全价饲料的饲养效果却起着极其重要的作用。科学的预混合饲料配方是预混料生产的关键。要生产出优质的预混料,不仅需要科学的配方,还需要     

拓展缉毒犬使用效果相关问题探析

一、当前国内缉毒犬使用情况 当前国内禁毒形势日益严峻,随着警犬作为装备配发政策落实程度的不断深化,一些地市公安机关相继建立起适应当地工作需要的警犬队伍,其中不乏缉毒犬队伍的发展与壮大,在此基础上缉毒犬使用也得到了较大程度的发展.总体体现在缉毒犬队伍专业化、规模化;缉毒犬使用日常化;参与缉毒专项行动、公开查缉等工作中也取得了较好成绩.     

训犬员,警营里的一道多味麻辣汤

在云南德宏边境一线,常年活跃着这样一群年轻士兵,他们身穿橄榄绿,终日与犬为伴。巡逻路上,他们带着警犬跋山涉水,风餐露宿,把足迹踏遍边境线上的每     

Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens

Four hundred male chickens were selected to study the effects of pyruvate (Pyr), creatine pyruvate (CrPyr) and creatine (Cr) on the expression of hepatic mitochondrial and cytoplasm proteins associated with lipid and protein metabolism. Mitochondrial purification was accomplished using the two-step differential centrifugation and density gradient method, and the activities of organelle-specific marker enzymes were determined to assess the purity of the mitochondria. Proteins were extracted and fractionated by two-dimensional electrophoresis and the differential protein spots were assessed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. CrPyr reduced fatty acid accumulation by down-regulating adipose differentiation-related protein, inhibited ATP synthase expression, and reduced cholesteryl ester transfer protein (CETP) expression, thus reducing the levels of high density lipoprotein and triglycerol (TG) levels (thereby lowering fat and cholesterol deposition). CrPyr increased the expression of eukaryotic translation initiation factor (eIF) 2B, calreticulin (CRT) and eIF3a, thus promoting protein synthesis. CrPyr up-regulated the expression of fatty acid-binding proteins, CETP and apolipoprotein A-IV in cytoplasmic extracts, and these proteins accelerated the decomposition of fatty acids and TG, thus reducing fat deposition. In conclusion, CrPyr plays an important role in lipolysis and protein synthesis, and this effect was more pronounced than was the effect of Pyr and Cr.     

频频变脸的中国饲料营销(1)

大饲料集团卖的啥20世纪80年代末和90年代初,这一段时间内中国产生的暴发户之多,真可谓是空前绝后。我国的饲料业就是在这个时候萌芽的,由于饲料行业科技含量低,投资门槛可高可低,营销简单易行,设备可繁可简,最简单时可简单到一把铁锹……一夜之间,一些民企经营高手们逢时而起,像天女散花似的,把饲料厂散遍大江南北,长城内外,造就了不少下辖相当数量子公司的饲料集团公司。在四川,首先是希望集团的崛起。刘氏四兄弟中老三——刘永美(陈育新)是学畜牧专业的,他从养鹌鹑、孵化小鸡开始,然后到为养殖户着想,提供长得快、不生病的饲料,就这样不…     

禽流感(H9)疫苗免疫间隔期探讨

通过在不同日龄对海兰褐蛋鸡进行禽流感(H9)灭活疫苗的免疫,探讨禽流感灭活疫苗的免疫间隔期。结果表明,开产以后不管防疫2次、3次、4次禽流感灭活疫苗,抗体水平均在6log2以上,且鸡的日龄越大,免疫次数越少,免疫抗体持续期越长。因此,建议从育成到鸡群淘汰的饲养全程中,四次时机恰当、搭配合理的禽流感(H9)疫苗免疫基本上能使得95%以上产蛋期抗体维持于7log2以上。     

奶牛环形泰勒焦虫病诊治报告

２０００年６月上旬至７月下旬，我县营子、七贤等乡镇发生一种呈地方性流行或散发的急性奶牛疾病，以高热稽留、体表淋巴结肿大、贫血为主要特征。根据流行病学、临床症状、剖检变化、实验室诊断，确诊为奶牛环形泰勒焦虫（Ｔ．ａｎｎｕｌａｔａ）病，现报告如下：１流行病学从６月上旬开始陆续发病，发病率达２５％，其中死亡２头。该病以一胎母牛多发。经调查，多为带犊由外地购进，本地饲喂时间不足一年。检查牛体，能发现吸饱血或未吸饱血的豆粒大或米粒大牛蜱。２临床症状病牛初期体温上升高达４１℃，稽留热。肩前和鼠蹊淋巴结肿大，…     

由训练记录知美军军犬训练——《美军军犬作战条令》解读系列二

美军军犬训练十分注重过程跟踪和数据积累,每头军犬都建立了详实的训练使用档案,从其出生到死亡或退役,每天都记录军犬的训练使用科目、时间和饮食状况,并填写训练存在的问题和改进措施,每周至少安排24小时执行非训练任务,每月进行一次甚至两次体检。每头美军军犬训练使用记录都包括两类：一类是巡逻训练使用记录;     

Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus

Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found (₁VCKKITEHERCHVNI₁₅), (₂₀AFFGVMPRGTTPRAPVR₃₆), (₄₆RRGLETGWAYTHQGGI₆₁), (₂₈₁EGDMATGITYASYGYFC₂₉₇), (₄₂₆YSGEDPANLRVVTSQSPYVVVATNAIESGV₄₅₅) and (₄₈₁FIVTGLKRMAVTVGEQA₄₉₇) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody–antigen reaction of the non-structural proteins and may aid in the clinical application as well.