体积置换法直接测量土壤质量含水率及土壤容重

土壤含水率直接测量是相关研究和应用的基础,在土壤力学、作物栽培、农田灌溉、生态环境等研究和实践中十分重要。该文提出了一种与传统烘干称质量法相当的体积置换法直接测量土壤质量含水率及土壤容重。该方法在假设一定土壤颗粒密度的前提下,用一定体积的标准取样环刀取得土样后,通过向待测量土体补充水分使土壤达到饱和,用一定体积的水置换土壤中的充气空隙,直到土样达到饱和状态;再通过测量得到的初始/原始土样质量、饱和后土壤的质量以及已知土壤颗粒密度和水密度,计算得到被置换的充气空隙的体积,进而由此计算得到土壤质量含水率和土壤容重。采用3种不同土壤,即陕西杨凌黏黄土、北京粉壤土和江西黏红土,分别预配制成7种不同初始土壤体积含水率,含水率约为:风干土(含水率2%～3%)、5%、10%、15%、25%、30%和饱和含水率,以及3种不同土壤容重:1.25、1.35和1.45g/cm3进行室内试验。用类似的土样,采用传统方法烘干土样8、12、24、48h后,测量确定土壤的质量含水率,通过延长烘干时间测得数据表明,传统方法烘干8h所测得的质量含水率仍有1%～3.2%的含水率误差。最终试验结果表明体积置换方法测得的土壤含水率比传统烘干土样8h所测得的结果大2%～3%,比烘干土样48h所测得的结果大1%左右。体积置换方法测量操作过程简单,耗时较少,节约能源,测量结果具有较高精度。     

基于Modelica语言建模的柴油机稳动态性能仿真与试验

针对传统柴油机建模效率不高、所建模型抽象不易理解、软件平台开放程度差、模型通用性低的问题,采用多领域统一物理建模规范Modelica语言和面向对象的建模思想建立了柴油机的仿真模型。以上柴D4114B型发电用柴油机为例,在解决上述问题的同时模拟了负荷特性下柴油机的稳态过程,最高压力、排温等稳态数据点的实测值与仿真值的最大相对误差为8.9%,证明了所建立的柴油机模型具有较好的稳态性能。在此基础上进行了动态仿真,模拟了转速、单缸循环油量等参数的动态变化规律,并将部分参数的仿真值与实测值进行比较,两者吻合得比较好,证明了所建立的柴油机模型能较好地预测其动态性能,可为后续的基于Modelica语言的柴油机建模提供参考。     

饲料用秸秆丝化多频快速压缩成型工艺参数优化

为了进一步探明丝化后干玉米秸秆压缩成型的最佳工艺参数,利用WDW-200微机控制电子式万能试验机,以秸秆含水率、最大压缩力(能够压缩成型的最小压缩力)、压缩次数和压缩速度为影响因子,对饲料用玉米秸秆丝化后压缩成型块的松弛比进行了二次回归旋转组合试验研究。建立并分析了4个影响因子对玉米秸秆丝化压缩成型松弛比的回归模型,并进行了验证试验。结果表明:丝化后玉米秸秆压缩成型最佳工艺条件为:含水率为7.8%~18.3%,能够成型的最小压缩力为6.22 MPa,压缩次数为2~6次,压缩速度为128~337 mm/min。在此工艺条件下,干玉米秸秆丝化压缩成型的松弛比在1.2和1.5之间,均能满足实际生产中较低能耗与生产质量的要求。该试验对压缩工艺的优化、压缩设备的研制和了解压缩成型后产品的特性具有参考意义。     

基于土地功能的农村居民点内部用地结构分类

科学合理的农村居民点内部用地功能结构分类是提升农村土地利用效率、实现用地结构优化配置的重要前提。该文在梳理农村居民点用地分类现状的基础上,从土地利用的功能角度出发,构建了涵盖农村居民点用地生产功能、生活功能、生态功能和潜在功能的分类体系,其中包含4个一级类、10个二级类和37个三级类;并以该分类方法为基础,通过实地调查典型农村居民点内部用地结构现状,从微观视角揭示内部用地结构。基于新的分类体系,大兴庄村用地类型可分为28种,农村居民点内部生产用地、生活用地和生态用地的比例分别为34.73%、53.53%和4.06%,可挖潜面积比例为占农村居民点总面积的13.09%。研究结果为中国农村居民点用地分类体系构建提供了基本思路和工作框架。     

单轨道山地果园运输机齿条齿形优选

为减小单轨道山地果园运输机能耗及提高运输效率,该文基于动力学理论建立了运输机驱动轮与轨道齿条啮合的动力学模型,并设计、加工制造了链轮齿形齿条、销轮齿形齿条、摆线齿形齿条。以运输机驱动轮旋转角速度、轨道坡度、齿条齿形为考察因素,以驱动轮与不同齿形齿条啮合时所需提供的驱动扭矩为评价指标,探究齿条齿形对单轨道山地果园运输机力学性能的影响,得到在相同条件下驱动轮与链轮齿形齿条啮合时的驱动扭矩最小,且波动幅度最小。在驱动轮转速为+88.08 rad/s、轨道坡度分别为+0?、+6?、+12?时,驱动轮与链轮齿形齿条啮合时的驱动扭矩均值较驱动轮与圆弧齿形齿条啮合时的驱动扭矩均值分别减小33.82%,33.45%,21.59%;在驱动轮转速为-88.08 rad/s、轨道坡度分别为-0?、-6?、-12?时,驱动轮与链轮齿形齿条啮合时的驱动扭矩均值较驱动轮与圆弧齿形齿条啮合时的驱动扭矩均值分别减小35.55%,27.24%,30.43%。试验结果表明,链轮齿形齿条综合性能最优,较圆弧齿形齿条更适宜用于单轨道山地果园运输机的轨道运输中。该研究为单轨道山地果园运输机轨道的结构优化设计提供了参考。     

高速电磁阀电磁力近似模型的构建与分析

为提高高速电磁阀动态响应速度,采用近似模型方法,以建立电磁阀多物理场零维近似耦合模型,实现其性能高效预测及优化。首先创建了柴油机电控单体泵高速电磁阀电磁力有限元计算模型,并通过与试验对比验证了模型的精度。结合面中心复合设计、嵌套中心复合设计、最优拉丁超立方设计与二次多项式响应面模型、Kriging模型、径向基函数模型,构建了18组电磁力近似模型。分析了不同样本点集大小、试验设计方法及近似方法对近似模型精度的影响。得出近似模型的精度随着样本点集的增大并非呈现单调递增的关系;而最优拉丁超立方试验设计与Kriging模型、径向基函数模型具有良好的适应性。构建高速电磁阀工作气隙、驱动电流、线圈匝数、副磁极半径、衔铁厚度、衔铁半径等关键参数的电磁力近似模型最佳方案是最优拉丁方试验设计与Kriging模型的组合,样本点集大小为二次多项式响应面模型所需最少样本点数的1.5倍,模型复相关系数、平均绝对误差、均方根误差值分别为0.97、0.06、0.09。该研究为高速电磁阀多物理场零维近似耦合模型的建立及其优化提供了参考。     

Hydroxycinnamoylmalic acids and their methyl esters from pear (Pyrus pyrifolia Nakai) fruit peel

Two novel caffeoylmalic acid methyl esters, 2-O-(trans-caffeoyl)malic acid 1-methyl ester (6) and 2-O-(trans-caffeoyl)malic acid 4-methyl ester (7), were isolated from pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) fruit peels. In addition, 5 known hydroxycinnamoylmalic acids and their methyl esters were identified: 2-O-(trans-coumaroyl)malic acid (1), 2-O-(cis-coumaroyl)malic acid (2), 2-O-(cis-coumaroyl)malic acid 1-methyl ester (3), 2-O-(trans-coumaroyl)malic acid 1-methyl ester (4), and 2-O-(trans-caffeoyl)malic acid (phaselic acid, 5). The chemical structures of these compounds were determined by spectroscopic data from ESI MS and NMR. Of all the isolated compounds, five hydroxycinnamoylmalic acids and their methyl esters (2-4, 6, 7) were identified in the pear for the first time.     

Effect of Preharvest Sprays of Calcium on Cracking and ‘Schattenmorelle’ Sour Cherry Fruit Quality Harvested Mechanically

The aim of the study was to examine effect of preharvest sprays of calcium (Ca) in the form of Ca-chloride (CaCl₂), Ca-nitrate [Ca(NO₃)₂], or a mixture of Ca-formate, Ca-acetate, CaCl₂, and Ca(NO₃)₂ on cracking and quality of ‘Schattenmorelle’ sour cherry fruit harvested mechanically. The experiment was conducted in 2008–2009 at a commercial orchard in central Poland. Mature trees grew on a coarse-textured soil poor in organic matter, at a spacing of 4.0 × 1.5 m. The spray treatments of Ca were performed at 7-day intervals, starting 28 days before harvest, at the rates of 5.0–5.6 kg Ca ha^?1 per season. The trees sprayed with water were treated as the control. Fruit were harvested mechanically when peduncle-fruit detachment force dropped below 3 N. The results showed that preharvest Ca sprays caused no leaf damage. This measure did not affect yield, mean fruit weight, soluble solids concentration and titratable acidity of fruit, and weight loss of fruit during 24 h after harvest. Fruit sprayed with Ca had improved status of this nutrient, and were less liable to juicy leakage from the stem scar, rain-induced cracking, and preharvest decay caused by Glomerella cingulata. The above effects of Ca sprays did not depend on the tested material. It was concluded that preharvest sprays of Ca as CaCl₂ and/or Ca(NO₃)₂ should be recommended in ‘Schattenmorelle’ sour cherry orchards to reduce fruit losses resulting from rain-induced cracking, leakage of juice, and the incidence of cherry bitter rot.     

人工合成抗菌肽D2A21基因的克隆和表达

为了获得高效表达的抗菌肽D2A21,本研究根据抗菌肽D2A21的氨基酸序列及大肠杆菌偏爱的密码子,设计并人工合成D2A21基因,并将该合成基因克隆至pProEXHTb载体中,构建成含有含6个组氨酸标签的重组质粒。重组质粒转化至大肠杆菌中,经摇瓶发酵,IPTG诱导后,发酵液用聚丙烯酰胺凝胶电泳分析。结果表明,成功合成D2A21基因,并构建了抗菌肽D2A21表达载体。通过镍柱亲和层析纯化,聚丙烯酰胺凝胶电泳分析,抗菌肽D2A21能够在大肠杆菌中稳定表达。本研究将为大规模表达纯化D2A21及其进一步的研究和利用提供一定基础。     

Biochar derived from dairy cattle carcasses as an alternative source of phosphorus and amendment for soil acidity

Two pot experiments were conducted to evaluate biochar derived from dead dairy cattle as a mineral fertilizer, especially phosphorus (P) fertilizer, and to clarify the effect of particle size of biochar on plant growth (Zea mays L.) and P uptake. To produce the biochar, body parts of dead cattle were placed in a charring chamber and allowed to char at 450°C for 4 h. The biochar was of high pH and rich in major plant nutrients, especially P. Application of fine biochar (< 1 mm) increased P uptake by the corn plants grown in soil of low available P status. As a result, plant growth was improved following biochar application and dry matter production was also increased. The effect of the biochar application on the P uptake and plant growth was promoted by the application of mineral nitrogen (N) fertilizer. Soil analysis after harvest indicated that the biochar application increased soil pH, available P and exchangeable calcium (Ca) and magnesium (Mg) compared with the soil before seeding, while soil available N and exchangeable potassium (K) were considerably decreased. The decrease in the soil available N was incomprehensible, because the result of the mass balance given by the difference between input as the applied N from the biochar and fertilizer N and output as the N uptake by the plants was positive. We observed a similar result in the mass balance of K to the case of N. The medium (2–4 mm) and coarse (> 4 mm) grade biochar did not significantly affect plant growth, because P uptake was not, or was only slightly, increased by the application of these biochars. Dissolution of P from the coarser biochars was probably slower than that of the fine biochar. The lower dissolution of P from the medium and coarse biochars was supported by the lower P absorption efficiency of these biochars compared with that of the fine biochar and superphosphate. The effect of fine biochar on plant growth and P uptake was similar to that of superphosphate. We can therefore conclude that fine biochar derived from cattle carcasses is an effective source of P fertilizer and amendment for soil acidity. The N and K contents in the biochar, although relatively high, cannot be relied upon as a mineral fertilizer. Further studies are needed to assess whether the N and K contents of the biochar indicate it can be regarded as a useful fertilizer.