Effects of salinity and temperature on artificial cultivation and early ontogeny of giant kokopu,Galaxias argenteus (Gmelin 1789)

Attempts at artificial propagation and rearing of juvenile galaxiids (‘whitebait’) have been hindered by low fecundities and survival. To circumvent these issues, we subjected wild and captivity‐acclimated giant kokopu (Galaxias argenteus), a hitherto unexplored galaxiid, to captive breeding protocols and evaluated the effects of salinity and temperature on egg fertilisation, incubation duration and hatching. Eggs sourced over several spawning seasons were subjected to different salinity (0, 9, 15, 20 and 30–35 PSU) and temperature (4, 10, 15 and 17°C) combinations. Average fertilisation rates were highest (95%) when gametes were activated in fresh water (0 PSU) and declined as salinity increased. Average hatch rates were highest (71–68%) when eggs were incubated in fresh water (0 PSU) at 10°C and decreased as salinity and temperature increased. Sequential stages of embryonic development were documented and several year classes of offspring were produced to form a captive breeding colony. We conclude that the giant kokopu has notable reproduction‐related qualities that may be useful for future aquaculture exploitation of this species.     

La valeur ornithologique des zones humides de l'est Algerien

In the environs of the town of El Kala in northeast Algeria there are some extensive wetlands of great ornithological, and probably also other biological, importance. The most important are ‘Lake’ Tonga, a marsh of about 2700 ha with much Scirpus lacustris; Lake Oubeïra, a freshwater lake of about 2200 ha; Lake Mellah, where fresh water from brooks and sea water meet, of about 800 ha; Garaet el Mekhada, a Scirpus maritimus marsh of about 5600 ha and swamp forests, which could not be investigated.Many species of waterfowl were seen in the second half of May, 1976. From these and some other observations probable breeding may be derived in many cases. Important species in the region are: squacco heron Ardeola ralloides (about 100 seen), cattle egret Bubulcus ibis (about 2000 seen), little egret Egretta garzetta (about 65 seen), grey heron Ardea cinerea (rare), purple heron Ardea purpurea (about 60 seen, possibly far more present), little bittern Ixobrychus minutus, bittern Butaurus stellaris and night heron Nycticorax nycticorax.Beside herons, the following species were seen: ferruginous duck Aythya nyroca (about 100 seen, probably far more numerous and hidden by the vegetation), white-headed duck Oxyura leucocephala (some 50 seen, probably present in greater numbers), marbled teal Anas angustirostris, purple gallinule Porphyrio porphyrio (common in Tonga). Interesting birds of prey in the region were griffon vulture Gyps fulvus (up to 30), marsh harrier Circus aeruginosus (rather numerous in Tonga), tawny eagle Aquila rapax (probably breeding on one place) and Eleonora's falcon Falco eleonorae (up to 5).As to probable breeding birds, Tonga has the greatest density of species and individuals, but other wetlands also have important aspects.Probably the most important lake for breeding birds, Fetzara (near Annaba), was drained in 1937. Marbled teal, ferruginous duck and purple gallinule were very common there, and white-headed duck, purple heron, squacco heron, night heron, little egret, spoonbill Platalea leucorodia, glossy ibis Plegadis falcinellus, grey-lag goose Anser anser, red-crested pochard Netta rufina and gadwall Anas strepera used to breed.A brief review is given of wintering waterfowl, mainly based on counts by different observers in the period 1971–1979. Large numbers of waterfowl have been seen in Lake Oubeïra; up to 5000 pintail Anas acuta, up to 14000 wigeon Anas penelope, up to 27000 pochard Aythya ferina, up to 9000 tufted duck Aythya fuligula and up to 32000 coot Fulica atra, up to 850 black-necked grebe Podiceps nigricollis and up to 2300 little grebe Tachybaptus ruficollis.In Lake Mellah up to 9000 pochard have been seen, up to 12000 tufted duck, and up to 35000 coot.In Garaet al Mekhada up to 25000 wigeon, up to 5000 coot and up to 8000 grey-lag geese have been seen. It seems quite possible that the 95000 wigeon seen by Smart in the artificial lake of the Barrage de la Cheffia in 1974 fed in Mekhada. An insight of waterfowl averages during the different counts can be obtained from the reports, mentioned in the references.All large wetlands mentioned have a high, actual or potential, ecological value. Each of them deserves protection and, from the ecological viewpoint, Fetzara merits inundation. Protection should be aimed at the preservation of the special character of each of the wetlands and of their mutual relationship for birds.The region is the most important in Algeria for wintering duck and coot (an average of 93000 from 1974–1978) and probably also for breeding waterfowl (especially Tonga) in spite of the high quality of La Macta in West Algeria.For the preservation of this diversity, several factors are essential, e.g., water level, water quality, absence of exotic elements in the water fauna and a relatively low shooting pressure (with little disturbance). It is important to prevent drainage of the lakes and of their surroundings, afforestation of the adjacent land, deforestration and erosion in the catchment areas, introduction of exotic fish species—and it is important to introduce good rules concerning shooting.This complex of wetlands is part and parcel of an extensive area of high biological interest, of which the marine habitats and large semi-natural forests are also remarkable.It is to be hoped that this entity will be efficiently protected in the framework of the National Park of El Kala Project.     

Confirmation of declared provenance of European extra virgin olive oil samples by NIR spectroscopy

The potential of near-infrared transflectance spectroscopy (1100-2498 nm) combined with chemometric techniques to confirm the geographical origin of European olive oil samples was evaluated. In total, 913 extra virgin olive oil samples (210 Ligurian and 703 non-Ligurian) were collected over three consecutive harvests (2005, 2006, and 2007). A multivariate spectral fingerprint for Ligurian olive oil was developed and deployed to confirm or refute a claim that any given sample was Ligurian. Samples were pseudorandomly split into calibration (n = 280) and validation sets (n = 633); the only selection constraint applied was to insist on equal numbers of Ligurian and non-Ligurian samples in the calibration set. Following preliminary examination by principal component analysis, the full spectrum modeling method applied to the spectral data set was discriminant partial least-squares regression; various data pretreatments were also investigated. The best models correctly predicted the origins of samples in the prediction set up to 92.8 and 81.5% for Ligurian and non-Ligurian olive oil samples, respectively, using a first-derivative data pretreatment. The potential of this approach in commercial traceability and quality assurance schemes is noted.     

Identification of toxigenic Pasteurella multocida in atrophic rhinitis of pigs by in vitro characterisation

Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.     

Influence of grape-harvesting steps on varietal thiol aromas in Sauvignon blanc wines

The intense tropical fruit aroma of Sauvignon blanc wines has been associated with the varietal thiols 3-mercaptohexanol (3MH), derived from odorless precursors in the grape, and 3-mercaptohexyl acetate (3MHA), arising from 3MH during fermentation. Grapes and juice were sourced from five locations in Marlborough, New Zealand, taking hand-picked grapes and samples at four stages during the mechanical harvesting process and pressing, which were then fermented in replicated 750 mL bottles. With each set of juices, the highest concentrations of Cys-3MH and Glut-3MH were found in the juices pressed to 1 bar, but these juices produced wines with lower 3MH and 3MHA concentrations. With three of the juices, there was an increase in varietal thiol content for wines made from juices that had been machine harvested compared to the hand-picked samples, which matched earlier findings of lower 3MH and 3MHA levels in wines made from hand-picked grapes. Juices that were more oxidized, and which showed a higher absorbance at 420 nm, were found to produce wines with lower 3MH and 3MHA concentrations.