Evaluation of the chicory inulin efficacy on ameliorating the intestinal morphology and modulating the intestinal electrophysiological properties in broiler chickens

Chicory (Cichorium intybus) belongs to plants of the Compositae family accumulating energy in the form of inulin fructan. Chicory, a prebiotic, is a fermentable oligosaccharide and oligofructose that may affect the intestinal mucosal architecture and the electrophysiological parameters. Therefore, this study was conducted to evaluate the effectiveness of adding chicory fructans in feed on the intestinal morphology and electrogenic transport of glucose in broilers. Four hundred, 1 day old broiler chicks were randomly divided into two groups (200 bird per group) for 5 weeks. The dietary treatments were (i) control, (ii) basal diets supplemented with the dried, grinded ground chicory pulp containing inulin (1 kg of chicory/ton of the starter and grower diets). In duodenum, dietary chicory increased the villus height and villus width and villus height to crypt depth ratio (p < 0.05), but the duodenal crypt depth remained unaffected (p > 0.05). However, in jejunum, the villus height, crypt depth and villus height to crypt depth ratio were decreased by dietary chicory compared with control birds (p < 0.05). In ileum, the villus height and villus crypt depth was decreased by dietary chicory supplementation compared with control (p < 0.05), but, the villus height to crypt depth ratio was increased (p < 0.05). Moreover, dietary chicory relatively affected the electrophysiological parameters of the intestine but did not reach significance. The amount of ΔIsc after d ‐glucose addition to the jejunal mucosa was numerically higher for chicory fed birds (19 μA/cm²) than control birds (10 μA/cm²). The percentage of increase in the Isc after d ‐glucose addition (ΔIsc %) was higher for chicory group upto (90%) of the control group. In colon, the actual Isc value and Isc after d ‐glucose addition was numerically higher for chicory fed birds than control birds (p > 0.05). Moreover, the conductance of jejunal and colonic tissues after d ‐glucose addition remained unaffected by the dietary chicory. In conclusion, addition of chicory to broilers diet increased the duodenal villus height, villus width and villus height to crypt depth ratio and decreased the villus height and crypt depth in both jejenum and ileum. Furthermore, dietary chicory relatively modified the small intestinal electrogenic transport of glucose in broilers.     

Influence of Cultural Practices on the Performance of Long Level Basins in Egypt

Several field studies were conducted tobetter understand the influence of culturalpractices, inflow rate and field conditionson the performance of modern surfaceirrigation systems in Egypt. Field datawere collected on wheat and cotton underEgyptian conditions in order to estimateinfiltration, roughness and performanceparameters. Tests were made with a varietyof inflow rates and a variety ofcross-section shapes (flat or furrowed andseveral furrow spacings). These studiesprovide information on expected values ofinfiltration and roughness parameterstypical of cracking clay soils in the NileDelta, as well as guidelines andrecommendations for use of long-levelbasins in Egypt.     

Deoxynivalenol in chicken feed alters the vaccinal immune response and clinical biochemical serum parameters but not the intestinal and carcass characteristics

This study was conducted to investigate the impacts of deoxynivalenol (DON) feeding either alone or in combination with a microbial feed additive (MFA) on the immune response to a viral vaccine and serum clinical chemical parameters. Forty 1‐day‐old boiler chicks were weighed and randomly divided into four groups, 10 birds in each group: (i) control group fed with basal diet; (ii) DON group fed with basal diet artificially contaminated with 10 mg DON/kg feed; (iii) DON + MFA group fed with basal diet contaminated with 10 mg DON/kg feed and supplemented with 2.5 kg of MFA/ton feed; and (iv) MFA group fed with basal diet supplemented with 2.5 kg of MFA/ton feed. At 35 days of age, birds were slaughtered and blood was collected for investigating the antibody titre against infectious bronchitis virus (IBV) and clinical chemical parameters. The results showed that DON reduced (p = 0.032) the titre against IBV, decreased (p = 0.005) the level of alanine transaminase (ALT) (4.2 ± 0.5 U/l) compared with control birds (6.4 ± 0.5 U/l), increased (p = 0.002) the serum cholesterol concentration (144 ± 6 mg/dl) compared with their control counterparts (123 ± 5 mg/dl) and increased (p = 0.074) the amount of circulating triglycerides (62.25 ± 7.50 mg/dl) compared with controls (39.55 ± 4.74). These results indicate that dietary DON altered the humoral immune response to viral vaccine and affected the serum clinical biochemistry. However, DON in combination with MFA did not affect serum IBV titre. Taken together, DON in the feed of broilers produced an impairment of the success of IBV vaccine and affected the health of birds.     

Analysis of strigolactones,germination stimulants for striga and orobanche,by high-performance liquid chromatography/tandem mass spectrometry

A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using high-performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/microL of strigol and 0.5 pg/microL of sorgolactone could be quantified, whereas 1 pg/microL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively.     

Influence of Organic Manures on Soil Characteristics and Yield of Jerusalem Artichoke

ABSTRACT

The objective of this study was to study the influence of three organic manures, farm yard manure (FYM), poultry manure (PLM), and pigeon manure (PGM), on soil physical and chemical properties on tuber yield of Jerusalem artichoke in a newly reclaimed saline calcareous soil. A field experiment was conducted applying the three manures, alone and/or in different combinations. Soils were investigated at surface (0–30 cm) and subsurface (30–60 cm) layers before and after planting, and analyzed for physical and chemical properties. The results indicated that the application of 31.5 kg ha^?1 of PLM+10.5 kg of PGM T7 recorded highest available nitrogen, zinc, copper, and moisture content at the surface layer. The same results were obtained for iron and manganese at both layers. While, applying 21.0 kg ha^?1 FYM+21.0 kg ha^?1 PLM T10 recorded the best treatment for pH, phosphorus, zinc, copper, moisture content, and saturation percentage at the subsurface layer. Applying 21.0 kg ha^?1 PGM + 10.5 kg ha^?1 FYM+10.5 kg ha^?1 PLM T15 recorded the best treatment for organic matter content and bulk density at surface layer and reduced the electrical conductivity and inulin tuber content at both layers. On the other hand, calcium carbonate and sodium adsorption ratio were reduced in both layers by applying 21.0 kg ha^?1 PLM+10.5 kg ha^?1 FYM+10.5 kg ha^?1 PGM T14. The best treatment for tuber nitrogen content and total yield was obtained with applying 42.0 kg ha^?1 PLM T2 only and 31.5 kg ha^?1 FYM+10.5 kg ha^?1 PLM T4, respectively.     

Zinc and copper in some soils of Egypt as related to other soil properties

Total Zn in alluvial and calcareous soils (average 138 and 70 ppm respectively) was significantly related to their contents of CaCO₃ (negatively), O.M. and clay (positively). Extracting Zn by Na₂EDTA gave the highest values for both soil types. Total Cu contents varied widely from 26 to 111 ppm in alluvial and from 15 to 30 ppm in calcareous soils. They were negatively correlated with the CaCO₃ contents. The pot experiments showed that EDTA(NH₄)₂CO₃, Na₂EDTA and DTPA are reasonable extractants for available Zn from both soil types. DTPA was efficient for all soils investigated, while Na₂ EDTA and EDTA-citrate were specific for extracting Cu from calcareous soils.     

Protein Composition of Seminal Plasma in Fractionated Stallion Ejaculates

Seminal plasma (SP) contains several types of compounds derived from the epididymides and accessory glands. The aim of this study was to examine the protein composition of different ejaculate fractions. Trial I: fractionated ejaculates were collected from two normal and two subfertile stallions. Samples containing pre‐sperm fluid and the first sperm‐rich jets (HIGH‐1), the main sperm‐rich portion (HIGH‐2), the jets with low sperm concentrations (LOW), and a combined whole‐ejaculate (WE) sample was centrifuged, and the SP was filtered and frozen. A part of each SP sample was stored (5°C, 24 h) with spermatozoa from HIGH‐2 and skim milk extender. Sperm motility was evaluated after storage in extender mixed with the stallion’s own SP or SP from one of the other stallions (sperm from a normal stallion stored in SP from a subfertile stallion and vice versa). Protein composition was analysed using reverse‐phase liquid chromatography (RP‐HPLC), N‐terminal sequencing and mass spectrometry. The area‐under‐the‐curve (AUC) was used for quantitative comparison of proteins within fractions. Trial II: semen samples were collected from seven stallions. Fractions with the highest (HIGH) and lowest (LOW) sperm concentrations and WE samples were examined using SDS‐PAGE and densitometry. No significant differences emerged between fractions in the AUC‐values of the Horse Seminal Protein‐1 (HSP‐1) and HSP‐2 peaks, or the peak containing HSP‐3 and HSP‐4 (HSP‐3/4). Levels of HSP‐1, HSP‐2 and HSP‐3/4 were not significantly correlated with total sperm motility, progressive sperm motility or average path velocity after storage. Significant differences between ejaculate fractions in the amount of different protein groups present in SP were not found in Trial I; but in Trial II, the proteins in the 60–70 kDa range were more abundant in LOW than in HIGH and WE, indicating that this band contained proteins derived mainly from the seminal vesicles, which produce most of the SP in LOW.