Pathology of Brucella ovis infection in red deer stags (Cervus elaphus)

Abstract

AIM: To describe the pathology of the reproductive tract of red deer stags with active Brucella ovis infection and in stags in which B. ovis infection had resolved.

METHODS: Twenty-three red deer stags of varying history were slaughtered and their epididymides and accessory sex glands examined grossly and by histopathology. At the time of slaughter five of the stags had an active B. ovis infection of 24–55 days duration following exposure to infected rams, 10 stags had been experimentally infected with B. ovis by intravenous inoculation 649 days previously and had developed an active infection but the bacterial infection had resolved at least 308 days prior to slaughter, and eight stags had not been exposed to B. ovis at any time.

RESULTS: Of the five stags with an active infection, one had gross enlargement of the epididymides that could be detected by scrotal palpation. Histological lesions in all five stags included mild to severe, predominantly non-suppurative epididymitis, vesiculitis, prostatitis and ampullitis, with neutrophil exudation in associated glandular ducts. Additional lesions in the epididymides were spermatic granulomas and epithelial hyperplasia with intra-epithelial cyst formation. Of the 10 stags in which the bacterial infection had resolved, two had gross enlargement of the epididymides. The histological lesions were similar to those in stags with active infection but were generally milder, with increased periductal scar tissue in the epididymides. The lesions seen in stags resembled those seen in rams with B. ovis infection but they were usually less florid and had fewer plasma cells. No gross abnormalities or histopathological lesions were detected in the non-infected stags.

CONCLUSIONS: Only a small percentage of red deer stags infected with B. ovis develop lesions of epididymitis that can be detected by scrotal palpation. Gross and histological lesions of the genital tract of stags associated with B. ovis infection are similar to the lesions seen in rams. Lesions in stags persist for >300 days after the bacterial infection has resolved.

CLINICAL RELEVANCE: Brucella ovis infection should be considered when there are gross lesions of epididymitis or histological evidence of inflammation in the epididymides or accessory sex glands of red deer stags. Retrospective diagnosis of B. ovis in stags could be achieved by histological examination of the reproductive organs.     

Extended-altitude, aerial mapping of crop NDVI using an active optical sensor: A case study using a Raptor™ sensor over wheat

Recently reported testing of active, optical crop sensors in low-level aircraft have demonstrated a new class of airborne sensing system that can be deployed under any ambient illumination conditions, including at night. A second-generation, high-powered, light-emitting diode system has been assembled and tested over a 80 ha field of wheat (Triticum aestevum) by mapping the normalised difference vegetation index (NDVI) at altitudes ranging from 15 to 45 m above the canopy; significantly higher altitudes than existing systems. Comparisons with a detailed on-ground NDVI survey indicated the aerial sensor values were highly correlated to the on-ground sensor (0.79 < R² < 0.85), with close to unity slope and zero offset. The maximum average deviation between aerial and on-ground NDVI values was 0.04. Sample calculations involving two exemplar algorithms, one for estimating monoculture pasture biomass and the other for estimating wheat yield, indicate such deviations to have no significant effect on prediction accuracy. The subsequent NDVI maps proved to be invariant to sensor height over the 15-45 m altitude range meaning this new sensor configuration can be deployed over undulating crops and pastures and in areas with nearby obstacles such as trees and buildings.     

Ultra low-level airborne (ULLA) sensing of crop canopy reflectance: A case study using a CropCircle™ sensor

Operational airborne and satellite remote sensing in agriculture remains constrained by matching platform availability to suitable daytime weather and illumination conditions, crop development, and availability of ground staff. An ultra low-level aircraft carrying an active NIR/Red CropCircle™ sensor was successfully deployed to record and subsequently map crop vigour via the simple ratio (SR) index over a field of sorghum. Given the logging frequency of ≈20 Hz and the presence of alternate rows of bare soil, the Moiré effect reduced the contrast between crop and bare soil skip-rows. Such effects would not be expected to occur in non-skip-row crops. The ultra low-level airborne (ULLA)-SR map derived from the 20 m transect records compared favorably with the SR map derived from a meter-resolution airborne digital multispectral image that was re-sampled to a similar spatial resolution. This case study, involving a CropCircle™ sensor mounted in a low-level aircraft demonstrates another deployment option for users of this class of sensor. Moreover, an ULLA configuration offers the potential for greater flexibility in scheduling compared to airborne imaging, given it can be flown at any sun-angle, under cloud, at night, and may easily be incorporated into aircraft already conducting low-level operations, for example crop dusting and reconnaissance, over agricultural fields.     

Testosterone serum profile,semen characteristics and testicular biometry of Mangalarga Marchador stallions in a tropical environment

This study was conducted to characterize the daily profile of testosterone secretion and its mean concentrations in the four seasons as well as to evaluate the semen characteristics and testicular biometry of Mangalarga Marchador stallions throughout the year in a tropical region. Three stallions were submitted to semen collections and evaluation of testicular biometry every 14 days along a year. Blood samples were collected once at the middle of each season, in a 20‐min interval during 24 hr in order to evaluate the testosterone secretion profiles among seasons. Testosterone concentrations along the day were higher at the beginning of the afternoon (from 12:00 to 15:00 hr), but a circadian secretion was not clearly observed. Mean testosterone concentrations did not differ among seasons (p > .05), but a pattern of secretion along the day showed variations with higher concentrations in the afternoon during the winter. Ejaculate volume was higher during summer; however, sperm motility decreased in summer and spring. Total sperm in ejaculate, sperm morphology and testicular biometry kept constant along the year showing no differences among the seasons. The results demonstrated that in a tropical region, reproductive aspects of stallions did not show a clearly defined seasonal variation, and months of autumn and winter were not unsuitable for reproduction of the males.     

Traumatic injuries involving tendons of the distal limbs in horses: a retrospective study of 55 cases.

Fifty-five horses were presented to Colorado State University Veterinary Teaching Hospital between 1st of January 1980 and 31st of December 1989 for treatment of distal limb lacerations involving flexor tendons (n = 35) or extensor tendons (n = 20). Of the 35 flexor tendon lacerations, 11 horses were killed without treatment and 24 horses were treated. Twenty-two horses were included in determining outcome. Four (18 per cent) returned to their original level of use, nine (41 per cent) returned to limited riding, seven (32 per cent) returned to breeding or pasture soundness and two (9 per cent) were killed. Eighteen of the 20 horses with extensor tendon lacerations underwent treatment and 15 had sufficient follow-up to determine outcome. Seven (47 per cent) returned to their original or intended use, five (33 per cent) returned to limited use, one (7 per cent) was used for breeding and two (13 per cent) were killed. Results for horses with extensor tendon lacerations support previous reports that these injuries can be treated successfully. Results for horses having flexor tendon lacerations were better than in previous reports.