Immunologic mechanism of CXCL10 and its receptor involved in endometriosis

AIM: To investigate the immunologic mechanism of CXC chemokine ligand 10(CXCL10) and its receptor CXC chemokine receptor 3(CXCR3) involved in the process of endometriosis (EM). METHODS: Serum samples were collected from 3 groups: EM patients without operation (n=76), EM patients with operation (n=10) and the normal control persons (n=76). CXCL10 and CA125 concentrations were detected by means of ELISA and chemiluminometry. Cell surface antigens on the activated PBMC-CD3 and CXCR3, as well as CXCR3 subgene-CXCR3A and CXCR3B were tested by flow cytometry (FC) and RT-PCR when PBMC was separated from women with EM (n=10) and without EM (n=10), and then activated. RESULTS: Serum CXCL10 concentrations between three groups were significanly different (P<0.05). Compared to normal control group, although the supernatant CXCL10 concentration and CD3+/CXCR3+PBMC number in EM group has no significant difference (P>0.05), highly expressed CXCR3B in EM group rather than CXCR3A was observed. CONCLUSION: CXCL10 in women with EM is low, indicating that it plays a vital role in the process of EM and immune system of the women with EM is defected and impaired. The immunoreactivity of PBMC from both EM patients and normal person is same to activated signal, but the productions are different: PBMC in EM group mainly express CXCR3B but PBMC in normal person mainly express CXCR3A after activation, which may be one of the immune mechanisms that EM escapes from immunological lethal effect of the infected host.     

枣花发育过程中糖类物质的分布与变化

以壶瓶枣为试材,通过组织学制片,运用PAS-苏木精复染和汞-溴酚蓝法对材料进行染色,用显微镜观察了花芽形态分化过程及其花器官中的糖类物质分布。结果表明,从枣吊萌动到花开,淀粉粒在各个分生组织分裂最旺盛的细胞中逐渐积累;随着花器官的发育,在萼片中形成囊状特化大细胞,到花粉粒成熟时布满萼片、花瓣和花托,标志着糖类物质积累达到高峰;但是当花开时,这些囊状特化大细胞中的糖类物质消失,变成空腔。     

长输管道的试压

长输管道试压是管道施工中的一道重要工序,是对管材和焊接质量的综合检验。从管段划分、试压介质的选择、试验压力的确定等方面对试压工作进行了论述,介绍了国内外先进的施工实例和试压理念,旨在推动实现试压工作的专业化,保证管道长期运行的安全性。     

VES：阴道炎症（vaginitis）辅助诊断专家系统设计与实现

阐述基于规则诊断推理技术的阴道炎症辅助专家系统的设计与实现.针对阴道炎症医疗诊断的特点,讨论该系统在建立过程采用的关键技术,描述系统的结构框架、知识表示、推理方法和实现技术.整合数据库技术,人工智能技术及阴道炎症医疗诊断技术,为实现阴道炎症智能诊断系统提供有价值的探讨.     

圈养山羊添喂S25山羊浓缩料增重效果及效益分析

进行了圈养山羊添喂“山羊浓缩料”增重试验，结果显示：试验组羊群比对照组健康、少发病，平均每只增重分别比对照组Ⅰ和Ⅱ高38．1l％和131．37％，增重盈利效益分别比对照组Ⅰ和Ⅱ高181．71％和288．70％。     

刺楸大规格苗木培育技术试验研究

针对刺楸大苗培育过程中存在的关键技术难题开展栽培试验研究,总结出了不同规格刺楸苗木合适的培育密度和整形修剪技术方法。     

蒙古马血液蛋白(酶)遗传多态性及聚类分析

采用垂直平板不连续聚丙烯酰胺凝胶电泳技术对221匹蒙古马（包括乌珠穆沁马、乌审马、锡尼河马和巴尔虎马）的8种血液蛋白进行多态性检测。计算了各位点的基因型频率、等位基因频率、遗传杂合度，并通过聚类分析探讨了群体间的亲缘关系和遗传距离。结果表明：除α1-B糖蛋白（A1B）位点呈现为单态外，其余7个蛋白（酶）位点均在不同程度上表现出多态。8个蛋白（酶）位点的平均杂合度分别为：乌珠穆沁马0．3296，乌审马0．3056，锡尼河马0．3783和巴尔虎马0．3666。对Nei氏标准遗传距离进行聚类分析，发现巴尔虎马和锡尼河马之间的遗传距离最近，它们与乌珠穆沁马和乌审马的遗传距离相对较远。     

MEK-石脑油可回收稀释降粘输送工艺

概述了稠油的物性,分析了石脑油中加入MEK(甲基乙基酮)后对稠油的稀释降粘效果。介绍了MEK-石脑油可回收的稠油稀释降粘工艺流程,指出MEK-石脑油回收稀释降粘工艺虽比石脑油回收稀释降粘工艺增设了一套MEK回收装置,投资及运行成本略高,但其降粘效果较好,能够以较小的稀释比提高稠油的输送能力。     

Subcellular localization of ZNF580-EGFP fusion protein

AIM: To construct a eukaryotic expression plasmid for enhanced green fluorescent protein (EGFP) and ZNF580 fusion protein, and study its subcellular localization in the transfected MGC803 cells. METHODS: The primers were designed according to the cDNA encoding sequence of ZNF580 full-length open reading frame (1-172aa), ZNF580 amino terminus (1-93aa) and ZNF580 carboxyl terminus (94-172aa). The three cDNA segments of PCR were cloned into pGEM-T vector. Then they were subcloned respectively into plasmid pEGFP-C1 (enhanced green fluorescent protein). The subcellular localization of the fusion protein in MGC803 cells transfected with the vector was monitored by autofluorescence microscopy. RESULTS: Restricted enzymes analysis and DNA sequencing showed that the sequences of the pEGFP-ZNF580 (1-172), pEGFP-ZNF580 (1-93) and pEGFP-ZNF580 (94-172) transgenic plasmid were correct. The fusion proteins of EGFP-ZNF580 (1-172) and pEGFP-ZNF580 (94-172) were localized in the nuclei. CONCLUSION: The recombinant eukaryotic expression vector pEGFP-ZNF580 has been successfully constructed. The nuclear localization signal is within amino acid residues 94 and 172 of ZNF580 carboxyl terminus (C2H2 zinc finger domain).