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331.
The Pathogenesis of Reactive Systemic Amyloidosis 总被引:2,自引:0,他引:2
Stephen P. DiBartola DVM Merrill D. Benson MD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1989,3(1):31-41
The diagnosis of amyloidosis is based on the presence of extracellular tissue deposits of proteinaceous material that demonstrate a characteristic green color when stained with Congo red and viewed under polarized light. Several different proteins are amyloidogenic but, in domestic animals, spontaneously occurring systemic amyloidosis is reactive in nature and characterized by the presence of amyloid protein AA. This type of systemic amyloidosis may occur secondary to chronic inflammatory or neoplastic disease, but in many instances no predisposing disease is found. A sustained increase in the serum concentration of serum amyloid A protein (SAA) is necessary but not sufficient for the development of reactive amyloidosis. Other inherited and acquired host-related factors are likely to be important in the development of reactive amyloidosis because this condition develops in few patients with chronic inflammatory disease. The tissue tropism of amyloid deposits varies with the amyloid protein itself and species affected. The consequences of amyloidosis for the host depend upon the tissues involved and the response of these tissues to the presence of the amyloid deposits. In domestic animals, reactive systemic amyloidosis is nephropathic, leading to end-stage renal disease, and the clinical presentation is that of uremia. 相似文献
332.
DAVID SZENTIMREY DVM MVSc DAVID FOWLER DVM MVSc Diplomate ACVS GEOFFREY JOHNSTON BSc MD FRCSC ALEX WILKINSON MA MB BChir MSc FRCPC 《Veterinary surgery : VS》1995,24(3):215-225
An autogenous free vascular bone graft of the canine distal ulna was evaluated. The vascularity of the graft was based on a musculoperiosteal sheath supplied solely by the caudal interosseous artery and vein. Four autogenous heterotopic (ulna to tibia) vascular transfers were performed. Two avascular transfers were performed to provide baseline criteria from which the success of vascularized transfers could be assessed. Clinical lameness evaluation, serial radiographs, bone scintigraphy, and sequential fluorochrome bone labeling were performed after surgery in both vascular and avascular transfers. All dogs were free of lameness in the donor limb by the 26th postoperative day. Serial radiographs revealed rapid graft incorporation and hypertrophy in all vascularized grafts and severe bone resorption in nonvascularized grafts. Histology, microangiography, and evaluation of fluorochrome bone labels were performed 90 days after surgery to determine graft viability, incorporation and temporal remodeling patterns. Microangiography and fluorochrome assessment complemented the histological findings. Based on these findings the distal ulnar bone graft was determined to be both viable and structurally adequate for selected cases of long bone reconstruction. 相似文献
333.
BJÖRN P. MEIJ DVM Diplomate ECVS GEORGE VOORHOUT DVM PhD Diplomate ECVDI TED S.G.A.M. VAN DEN INGH DVM PhD Diplomate ECVP HERMAN A.W. HAZEWINKEL DVM PhD Diplomate ECVS JOHANNA W. VAN 'T VERLAAT MD PhD 《Veterinary surgery : VS》1997,26(4):295-309
Objective — Assessment of a microsurgical technique for transsphenoidal hypophysectomy in dogs.
Study Design — Prospective study using physical examination, pituitary function testing, computed tomography (CT), and histological examination at autopsy.
Animals or Sample Population — Eight laboratory beagle dogs.
Methods — Pituitary function was assessed before and at 10 weeks after hypophysectomy by combined administration of four releasing hormones (anterior pituitary), administration of haloperidol (pars intermedia), and infusion of hypertonic saline (posterior pituitary).
Results — CT imaging enabled accurate preoperative localization of the pituitary. Appropriate positioning and surgical technique facilitated exposure of the pituitary and its extraction without hemorrhage. Postoperative recovery was generally uncomplicated. None of die eight dogs had somatotropic, gonadotropic, lactotropic, melanotropic, or posterior pituitary responses to stimulation at 10 weeks after hypophysectomy. Four dogs (ACTH nonresponders) also had no cortico-tropic response and four (ACTH responders) had small but significant responses in the combined anterior pituitary function test. Adrenocortical atrophy was more pronounced in the ACTH nonresponders man in the responders. No residual pituitary tissue was found along the ventral hypothalamic diencephalon but nests of pituitary cells were found embedded in fibrous tissue in the sella turcica.
Conclusions — The surgical technique proved to be safe and effective. Microscopic nests of pituitary cells in the sella turcica may be responsible for residual corticotropic response to hypophysiotropic stimulation after hypophysectomy.
Clinical Relevance — The surgical technique may be used in the treatment of dogs with pituitary-dependent hyperadrenocorticism. The corticotropic response is the most sensitive criterion in assessing completeness of hypophysectomy in dogs. 相似文献
Study Design — Prospective study using physical examination, pituitary function testing, computed tomography (CT), and histological examination at autopsy.
Animals or Sample Population — Eight laboratory beagle dogs.
Methods — Pituitary function was assessed before and at 10 weeks after hypophysectomy by combined administration of four releasing hormones (anterior pituitary), administration of haloperidol (pars intermedia), and infusion of hypertonic saline (posterior pituitary).
Results — CT imaging enabled accurate preoperative localization of the pituitary. Appropriate positioning and surgical technique facilitated exposure of the pituitary and its extraction without hemorrhage. Postoperative recovery was generally uncomplicated. None of die eight dogs had somatotropic, gonadotropic, lactotropic, melanotropic, or posterior pituitary responses to stimulation at 10 weeks after hypophysectomy. Four dogs (ACTH nonresponders) also had no cortico-tropic response and four (ACTH responders) had small but significant responses in the combined anterior pituitary function test. Adrenocortical atrophy was more pronounced in the ACTH nonresponders man in the responders. No residual pituitary tissue was found along the ventral hypothalamic diencephalon but nests of pituitary cells were found embedded in fibrous tissue in the sella turcica.
Conclusions — The surgical technique proved to be safe and effective. Microscopic nests of pituitary cells in the sella turcica may be responsible for residual corticotropic response to hypophysiotropic stimulation after hypophysectomy.
Clinical Relevance — The surgical technique may be used in the treatment of dogs with pituitary-dependent hyperadrenocorticism. The corticotropic response is the most sensitive criterion in assessing completeness of hypophysectomy in dogs. 相似文献
334.
An American Cocker Spaniel with low plasma taurine concentration (< 2 nmol/mL) was presented with dyspnoea associated with pulmonary oedema and a left ventricular shortening fraction of 9%. Emergency therapy with furosemide, dobutamine, nitroglycerine and oxygen supplementation led to a good response. Chronic therapy was started with enalapril, furosemide, digoxin and taurine. Improvement in all echocardiographic indices were noted over a 22 week follow-up, most notably an increase in left ventricular shorteningfraction to 20%, a decrease of E-point septal separation from 14 mm to 7 mm and marked left ventricular remodelling. This degree of improvement in myocardial function may represent a direct link between dilated cardiomyopathy in the American Cocker Spaniel andplasma taurine deficiency. Alternatively, this response may reflect a breed-related cardiomyopathy with a natural history and therapeutic response not commonly seen in the morecommon large breed cardiomyopathy presentations. 相似文献
335.
RANDALL B. FITCH DVM MS RONALD D. MONTGOMERY DVM MS Diplomate ACVS JAMES L. MILTON DVM MS Diplomate ACVS PHILLIP D. GARRETT DVM MS STEVEN A. KINCAID DVM PhD JAMES C. WRIGHT DVM PhD GLENN C. TERRY MD 《Veterinary surgery : VS》1995,24(2):148-155
The intercondylar fossa (ICF) in dogs consists of a cranial outlet, intercondylar shelf, caudal arch, caudal outlet, a medial wall, and a lateral wall. The normal cranial outlet is bell-shape and, in mixed-breed dogs (mean body weight 19.2 kg, N = 21), measured 5.8 mm cranially, 8.1 mm centrally, and 10.3 mm caudally. The ICF is oriented 12° from the dorsal plane of the femoral diaphysis and obliqued 7°, proximolateral to distomedial, in the sagittal plane. To adjust for dog size, a fossa width index (FWI) was calculated by dividing the cranial outlet width by the distance between epicondyles. The normal FWI as determined in this study was 0.18 cranially, 0.25 centrally, and 0.32 caudally. The fossa height index was 0.31. Contact between the ICF and the cranial cruciate ligament began at about 115° of extension. The contact area moved cranially in the intercondylar fossa as the stifle was extended. Evaluation of the ICF can be performed radiographically but positioning is critical. 相似文献
336.
Knowledge of the presence or absence of lice in a flock of sheep enables wool growers to make informed decisions as to the need for insecticidal treatments. However, with inapparent infestations, traditional methods of detection are not sufficiently sensitive and, as a consequence, flocks may be left untreated. Conversely, the routine application of insecticide to sheep with no sign of infestation is an unnecessary cost. The sensitivity of 3 procedures for detecting lice was evaluated in 68 mobs of sheep from 50 farms. In 24 mobs of sheep known to be lightly infested, lice were detected in 17% by either parting the fleece of 10 sheep or by the lamp test in which 8 g samples of shorn wool from 30 randomly selected fleeces were placed under lamps for 10 min to repel the lice. Twenty of 23 mobs (87%) were found to be infested by the table locks test in which a 30 g sample of locks wool was dissolved in 10% sodium hydroxide and the filtered residue examined with x 40 magnification. A screening test, in which either fleeces on 5 sheep were examined by fleece parting or lice were repelled from 30 shorn fleeces for 5 minutes, detected about 60% of lightly infested mobs. When this was followed by the table locks test 91% of lightly infested mobs were detected. Conducting any one of the tests on more than one mob, and in large mobs testing more frequently, increases the sensitivity of detection of lice within the whole flock. 相似文献
337.
Michael G. Davidson DVM Edward B. Breitschwerdt DVM David H. Walker MD Mark P. Nasisse DVM Wendy E. Sussman BS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1989,3(1):7-11
Direct immunofluorescence reaction for Rickettsia rickettsii was performed on formalin-fixed, paraffin-embedded cutaneous biopsy specimens collected from dogs with experimental Rocky Mountain spotted fever (RMSF). A technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. R rickettsii was identified only in grossly evident dermal lesions (macular rash or oral vesicles) and was not apparent in randomly collected biopsy specimens from clinically normal inguinal skin. These results suggest that clinical application of this technique for diagnosis of RMSF may be limited in dogs without cutaneous lesions. (Journal of Veterinary Internal Medicine 1989; 3:8–11) 相似文献
338.
Sven-Erik Hamp DDS Odont dr Sten-Erik OlssonVet med dr MD Dr Sc h c Klaus Farsø-Madsen DVM Peters Viklands DVM Jan Fornell DDS 《Veterinary radiology & ultrasound》1984,25(2):86-92
The type and frequency of dental diseases and disorders were determined in 162 randomly selected dogs available for necropsy. There were 82 males and 80 females ranging in age from seven months to 14 years. There were 150 purebred dogs (50 different breeds) and 12 mongrels. Only four breeds were represented by ten individuals or more: Poodles (Miniature and Toy), German Shepherds, Dachshunds, and Boxers. The oral cavity was examined macroscopically, and missing teeth, dental caries, dental calculus (tartar), and gingival epulides were recorded as to frequency and distribution. After macroscopic examination of the oral cavity, the head of each dog was sawed at the midline, fixed in formalin, and radiographed. The frequency, severity, and distribution of marginal periodontitis, periapical destruction, and root resorption were recorded. The most frequently noted disease was periodontitis, which increased in frequency and severity with increasing age of the dogs. The disease varied markedly among and within different breeds. Small dogs were more often affected with periodontitis than large dogs. Periapical destruction was a common finding. Caries and root resorption were less common. Missing teeth was a frequent disorder regardless of age. Most of the dogs had dental calculus. 相似文献
339.
Bianca J.J.M. Brundel PhD Peter Melnyk PhD Lena Rivard PhD Stanley Nattel MD 《Journal of Veterinary Cardiology》2005,7(2):121
Atrial fibrillation (AF) occurs in dogs in a number of settings, the most common of which is congestive heart failure. This paper reviews what is known about the pathology of AF in dogs, as well as its clinical relevance. We begin by discussing several experimental AF paradigms in dogs, the associated pathology, and its relevance to AF mechanisms. We then discuss clinical AF in dogs and its relationship to experimental pathology. Finally, we conclude by assessing the potential therapeutic relevance of understanding AF-related pathology in dogs, as well as its potential to have practical applications in the future. 相似文献
340.
Effects of Serum Deprivation and Cycloheximide on Cell Cycle of Low and High Passage Porcine Fetal Fibroblasts 总被引:1,自引:0,他引:1
MD Goissis HVA Caetano MG Marques FRO de Barros WB Feitosa MP Milazzotto M Binelli MEOA Assumpção JA Visintin 《Reproduction in domestic animals》2007,42(6):660-663
Arrest of cells in G0/G1 cell cycle phase is desired for nuclear transfer procedures. Serum starvation and cell cycle inhibitors are different ways to induce synchronization of the cell cycle. This study investigated the effects of serum starvation and cycloheximide (CHX) on the cell cycle of low (5th) and high (15th) passages fetal porcine fibroblasts. Cell cycle phases were determined using fluorescent activated cell sorting. Fifth passage fibroblast cultures had higher (p < 0.05) proportion of cells in G0/G1 only after 72 h of serum starvation (77.60 ± 0.65) when compared with non‐starved cells (71.44 ± 1.88). Serum starvation for all periods tested induced an increase (p < 0.05) on proportion of cells in G0/G1 on the 15th passage. No significant differences were observed on the 5th passage cultures exposed to CHX, although, on the 15th passage an increase on proportion of cells was observed after all periods of exposure (p < 0.05). These data indicates that high passage cells in vitro are more susceptible to serum starvation and CHX G0/G1 synchronization. 相似文献