Laboratory evaluation of a living attenuated vaccine against bluetongue type 20 virus

Living attenuated bluetongue Type 20 virus vaccine was tested in 9 to 12 month-old Australian Merino sheep, held in air conditioned, insect-free accommodation. The vaccine appeared avirulent and immunogenic and protected against infection with a second dose of homologous vaccine virus. No enhancement of virulence or significant change in immunogenicity was observed when the vaccine was passaged 3 times through sheep without antibody to bluetongue virus.     

Spatiotemporal variations of evapotranspiration and reference crop water requirement over 1957-2016 in Iran based on CRU TS gridded dataset

Agriculture needs to produce more food to feed the growing population in the 21^st century. It makes the reference crop water requirement (WREQ) a major challenge especially in regions with limited water and high water demand. Iran, with large climatic variability, is experiencing a serious water crisis due to limited water resources and inefficient agriculture. In order to overcome the issue of uneven distribution of weather stations, gridded Climatic Research Unit (CRU) data was applied to analyze the changes in potential evapotranspiration (PET), effective precipitation (EFFPRE) and WREQ. Validation of data using in situ observation showed an acceptable performance of CRU in Iran. Changes in PET, EFFPRE and WREQ were analyzed in two 30-a periods 1957-1986 and 1987-2016. Comparing two periods showed an increase in PET and WREQ in regions extended from the southwest to northeast and a decrease in the southeast, more significant in summer and spring. However, EFFPRE decreased in the southeast, northeast, and northwest, especially in winter and spring. Analysis of annual trends revealed an upward trend in PET (14.32 mm/decade) and WREQ (25.50 mm/decade), but a downward trend in EFFPRE (-11.8 mm/decade) over the second period. Changes in PET, EFFPRE and WREQ in winter have the impact on the annual trend. Among climate variables, WREQ showed a significant correlation (r=0.59) with minimum temperature. The increase in WREQ and decrease in EFFPRE would exacerbate the agricultural water crisis in Iran. With all changes in PET and WREQ, immediate actions are needed to address the challenges in agriculture and adapt to the changing climate.     

Anaesthesia with ketamine/medetomidine in the rabbit: influence of route of administration and the effect of combination with butorphanol

Objective To compare the characteristics of anaesthesia induced with ketamine/medetomidine administered by the subcutaneous and intramuscular routes and to assess the effects of the addition of butorphanol to this combination. Study design Prospective randomised study. Animals Six female New Zealand White rabbits. Methods Rabbits were given one of four combinations of ketamine and medetomidine (K/M) either subcutaneously (SC) or intramuscularly (IM) on four successive occasions with a 7‐day interval between treatments. The dose combinations were; 15/0.25 mg kg^?1 SC; 15/0.25 mg kg^?1 IM; 15/0.5 mg kg^?1 SC, and 15/0.25 mg kg^?1 together with 0.4 mg kg^?1 butorphanol (K/M/B) SC. The effects of anaesthesia on arterial blood gas values and cardiovascular variables were recorded at predetermined time points. Toe and ear pinch reflexes were judged to determine the duration of surgical anaesthesia. Loss of the righting reflex was used to measure the duration of sleep time. Analyses used repeated measures analysis of variance. Results All groups lost the righting reflex and ear pinch response. Three animals in the groups that received K/M alone lost their toe pinch reflex, whereas four lost this reflex when given K/M/B. Time of onset of loss of the righting, toe and ear pinch reflexes did not differ significantly among the groups. The higher dose combination of medetomidine with ketamine and the combination of K/M/B produced a greater duration of loss of the ear pinch response than the lower dose of K/M administered by either route. No significant differences were found among the groups in the duration of loss of the toe pinch reflex. All animals developed a moderate bradycardia (mean heart rate <166 beats minute^?1) and moderate hypoxaemia (mean P_aO₂ < 6.0 kPa). Animals given butorphanol showed the greatest reduction in respiratory rate (31 ± 13 breaths minute^?1, p < 0.05) but this was not reflected in any significant differences in arterial PCO₂, PO₂ or pH among the groups. Conclusions Administration of K/M by the SC route produced equivalent effects in comparison to intramuscular administration. The addition of butorphanol increased the duration of anaesthesia, but produced a slight increase in the degree of respiratory depression. All dose rates resulted in hypoxaemia so oxygen should be administered when these combinations are used in rabbits. Clinical relevance Subcutaneous administration is both technically simpler and may cause less discomfort to the animal than IM injection, and so is preferred. The combination of K/M with butorphanol has relatively minor effects on the depth and duration of anaesthesia, so offers little advantage to the use of K/M alone.     

Pesticidal seed coats based on azadirachtin‐A: release kinetics,storage life and performance

BACKGROUND: Infestation of seeds by pests during storage leads to deterioration in quality. Seed coating is an effective option to overcome the menace. Unlike synthetic fungicidal seed coats, little is known of those based on botanicals. This study aims at developing azadirachtin‐A‐based pesticidal seed coats to maintain seed quality during storage. RESULTS: Polymer‐ and clay‐based coats containing azadirachtin‐A were prepared and evaluated for quality maintenance of soybean seed during storage. Gum acacia, gum tragacanth, rosin, ethyl cellulose, hydroxyethyl cellulose, polyethyl methacrylate, methyl cellulose, polyethylene glycol, polyvinyl chloride, polyvinyl acetate, polyvinyl pyrrolidone and Agrimer VA 6 polymers and the clay bentonite were used as carriers. The time for 50% release (t_1/2) of azadirachtin‐A into water from the seeds coated with the different coats ranged from 8.02 to 21.36 h. The half‐life (T_1/2) of azadirachtin‐A in the coats on seed ranged from 4.37 to 11.22 months, as compared with 3.45 months in azadirachtin‐A WP, showing an increase by a factor of nearly 1.3–3.3 over the latter. The coats apparently acted as a barrier to moisture to reduce azadirachtin‐A degradation and prevented proliferation of storage fungi. Polyethyl methacrylate, polyvinyl acetate and polyvinyl pyrrolidone were significantly superior to the other polymers. Azadirachtin‐A showed a significant positive correlation with seed germination and vigour, and negative correlation with moisture content. CONCLUSION: Effective polymeric carriers for seed coats based on azadirachtin‐A are reported. These checked seed deterioration during storage by acting as a barrier to moisture and reduced the degradation of azadirachtin‐A. Copyright © 2008 Society of Chemical Industry     

Ireland's Foreign‐Owned Technology Sector: Evolving Towards Sustainability?

ABSTRACT For some, Ireland's pursuit of an exogenous‐led development model has proved to be the cornerstone of recent economic success. Others point to recent high‐profile closures and argue that foreign‐owned operations are attracted to Ireland solely because of the advantageous tax breaks and lucrative grants scheme offered by the Irish government. We pay tribute to both arguments by pushing the level of enquiry beyond that of supply and backward linkages to try and gauge the actual performance of affiliates themselves. This brings some interesting facets of the Irish foreign direct investment scene to light. We highlight complexity of process, attainment of broader investment remits, and the emergence of a managerial class as integral to the ability of affiliates to adapt to and exploit organisational change. By examining 10 case studies and making use of media searches and company interviews, we highlight evidence of Ireland's largest technology transnational corporation affiliates showing positive performance advances. With these movements come, what we term, increased nodal significance of Irish operations within the global production network of their corporations. We argue against policy and theories that see these movements as linear and provide evidence of how some Irish operations have leveraged control and gained significant regional and global remits that have resulted in their growing significance, both in the corporation and in the country in which they are based. In the same line we argue that embeddedness in terms of supply linkages does not fit the Irish case and instead employ the term “network anchoring” of affiliates as they increase their nodal weighting through increased mandates.