Exposure of Ankole and crossbred cattle to theileriosis in Rwanda

Summary Susceptible Ankole (Sanga:Bos indicus/Bos taurus) and crossbred Ankole x Jersey (B. taurus) and Ankole x Sahiwal (B. indicus) cattle derived from a farm in Rwanda with no recent history of theileriosis, were infected withTheileria parva stocks from Rwanda either by feeding infectedRhipicephalus appendiculatus ticks on the ears, inoculation of tick derived stabilate or natural exposure to tick challenge. The Ankole cattle originated from local stock born and bred in East Coast fever (ECF) endemic areas of Rwanda. Disease, followed by spontaneous recovery, was observed in 49 of the 72 Ankole cattle after infection withT. parva (68%); the other 23 animals (32%) died of ECF. In contrast 21 of the 33 infected crossbred cattle (64%) died of ECF. It is concluded that the partialTheileria tolerance of the Ankole is, to a great extent, genetic. The basis of this partial tolerance seems to be their ability to limit the explosive multiplication of macroschizonts during the acute phase of the disease.

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Resumen Se infectó ganado Ankole susceptible (Sanga:Bos indicus/Bos taurus) y ganado cruzado Ankole x Jersey (Bos taurus) y Ankole x Sahiwal (Bos indicus) proveniente de una finca en Rwanda sin historia reciente de theileriosis, conTheileria parva procedente de Rwanda, mediante la adhesión en la oreja deRhipicephalus appendiculatus infectado, inoculación de estabilados derivados de garrapatas, o exposición natural a la enfermedad a través del vector. El ganado Ankole era originario de un área endémica de theileriosis en Rwanda. Se observó la enfermedad seguida de recuperación, en 49 de 72 animales Ankole infectados conT. parva (68%); los otros 23 animales murieron (32%). En contraste, 21 de los 33 animales cruzados infectados (64%), murieron. Se concluye, que la tolerancia parcial del ganado Ankole es de origen genético. Las bases de ésta tolerancia genética, parece debida a la habilidad para limitar en cierto modo la multiplicación explosiva de macroesquizontes, durante la fase aguda de la enfermedad.

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Résumé On a infecté du bétail sensible, de race Ankolé (Sanga:Bos indicus/Bos taurus) et des croisés Ankolé x Jersey (B. taurus) et Ankolé x Sahiwal (B. indicus), provenant d'une ferme au Rwanda sans cas récents de theilériose, avec des souches deTheileria parva du Rwanda, par l'application de tiques (Rhipicephalus appendiculatus) infectées sur les oreilles, par inoculation de stabilat de tiques infectées ou par l'exposition à un challenge naturel de tiques. La souche d'Ankolé était originaire du cheptel local Rwandais, né et élevé dans les régions où la theilériose est endémique. La maladie suivie par la guérison spontanée fut observée dans 49 des 72 Ankolés (68%), les autres 23 animaux (32%) ont succombé à la theilériose. Par contre 21 des 33 croisées infectées (64%) ont succombé à la theilériose. Nous concluons que la tolérance partielle des Ankolés contre la theilériose, est génétique. La base de cette tolérance semble être leur capacité de limiter la multiplication explosive des macroschizonts pendant la phase aigu de la maladie.

     

Isolation and purification of a diagnostic antigen for bovine cysticercosis by hydrophobic chromatography

An ammonium sulfate fraction of Taenia hydatigena cyst fluid (ThFAS) was further fractionated by hydrophobic interaction chromatography, using alkylagarose and omega-amino alkylagarose columns, in an effort to isolate and purify a specific diagnostic antigen in the ThFAS preparation. The less than 12 kDa antigen was found to have an affinity for immobilized alkanes with chain length of six carbons or greater. The antigen was recovered in an ethylene glycol eluate from a hexylagarose column then analyzed by Western blot; it reacted with bovine and human cysticercosis infection sera and with specific monoclonal antibodies but not with control sera or fascioliasis infection sera. When the eluate was used as coating antigen in a plate ELISA assay no false positive reactions were seen in sera from cattle infected with Fasciola hepatica; false positive reactions were observed for the unfractionated ThFAS antigen preparation.     

Free-running rhythms of adrenocorticotropic hormone (ACTH), cortisol and melatonin in pigs.

In the domestic pig, a circadian rhythm of plasma cortisol occurs, with greatest concentrations in the morning and lowest concentrations in the afternoon. However, photic entrainment of the rhythms of ACTH and melatonin in pigs have not been defined clearly. This experiment was designed to evaluate free-running rhythms of ACTH, cortisol and melatonin in pigs housed in constant light (LL) and constant darkness (DD). Twelve crossbred barrows, maintained under ambient photoperiod, were catheterized and tethered individually in two environmentally controlled rooms, one with LL and the other with DD. For animals in LL, fluorescent lights provided 202 +/- 15 (mean +/- standard deviation) lux of light at 65 cm above the floors. Incandescent nightlights equipped with 7 watt red bulbs provided 7 +/- 2 lux and were illuminated continuously in both rooms. Pigs were given at least 14 d exposure to LL and DD, then samples of plasma and serum were obtained at hourly intervals for 48 hr. Plasma was assayed for ACTH, and serum for cortisol and melatonin. Periodograms were constructed to analyze the data. For this type of analysis, a statistic, Qp, is calculated, and circadian periodicity is suggested if maximum Qp (Qp max) occurs at or near 24 hr. The period of the free-running rhythms (tau) at Qp max for ACTH, cortisol and melatonin for pigs in LL (23.80 +/- .01, 23.78 +/- .01, and 23.21 +/- .02 hr, respectively) did not differ significantly from those for pigs in DD (23.39 +/- .01, 23.20 +/- .01, and 22.55 +/- .02 hr, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)     

山羊脑灰质软化症和李氏杆菌病的区别及防治方法

山羊脑灰质软化症 (ＰＯＬＩＯＥＮ ＣＥＰＨＡＬＯＭＡＬＡＣＩＡ)和李氏杆菌病 (ＬＩＳ ＴＥＲＩＯＳＩＳ)是症状相似治疗方法截然不同的两种疾病。山羊饲养者和兽医工作者必须从两种疾病的细微差别中进行鉴别 ,以便正确治疗和处理病羊。多数情况下 ,这两种疾病出现在饲养条件比较好、但饲养方法不当的地方。特别是在快速育肥时。精饲料喂量过多 ,而含粗纤维的饲料喂量过少是诱发两种疾病的重要因素。突然更换饲料常常导致此病突然发作。脑灰质软化症 (通常又称为大脑皮层坏死症 )是缺乏硫胺 (即维生素Ｂ1)而造成的一种代谢性疾病。瘤胃环…     

Double-blind evaluation of implants of gold wire at acupuncture points in the dog as a treatment for osteoarthritis induced by hip dysplasia.

Thirty-eight dogs with hip dysplasia were studied to evaluate the use of gold wire implants at acupuncture points around the hip joints. They were assigned at random into two groups of 19. In the treated group, gold wire was inserted through hypodermic needles at electrically found acupuncture points around both hips. In the control group, the areas were prepared in the same way but had only the skin pierced at sites which were not acupuncture points, with a needle of the same size as that used in the treated group. Over a period of six months the dogs were studied repeatedly by two veterinarians and by the dogs' owners who were unaware of the treatments the dogs had received; they assessed the dogs' locomotion, hip function and signs of pain. Radiographs were taken at the beginning and end of the study. Although the data collected from both groups by the veterinarians and the owners showed a significant improvement of locomotion and reduction in signs of pain (P=0.036 for the veterinary evaluation and locomotion and P=0.0001 and P=0.0034 for the owners' evaluation of locomotion and pain, respectively), there were no statistically significant differences between the treated and control groups (P=0.19 and P=0.41, P=0.24, respectively).     

The Effect of Size and Diet on Gonad Production by the Chilean Sea Urchin Loxechinus albus

Conadal production was studied in Loxechinus albus (Molina, 1782) maintained in cages suspended from a long-line in the Estero Castro, Chiloé for 3 mo during the austral summer. The sea urchins were fed either an artificial diet or a natural diet consisting of the macroalgae Macrocystis pyrifera and Ulva sp. Both diets were tested for four size ranges: 40–45, 50–55, 60–65 and 70–75 mm diameter. For all four size ranges, highest gonad production was with the artificial diet. Gonad production was greatest in the 40–45 mm individuals with an increase of about 1,400% and 750% in the wet weight of gonads in individuals fed the artificial and natural diet, respectively. With the three other size ranges, the increase was nearly 100% with the artificial diet and nearly 0% with the natural diet The gonad index showed similar patterns, being highest in the smallest individuals. Small individuals fed the artificial diet would provide the most cost effective aquaculture as production is best. It is not necessary to grow L. albus to the minimal legal size for fisheries (70-mm diameter) for cost effective gonad production.     

Characterization of and radioimmunoassay for canine thyroglobulin

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T₄, T₃, and DIT < 0.01 %).

Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T₄ levels.