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991.
Free amino acid composition of quince (Cydonia oblonga Miller) fruit (pulp and peel) and jam 总被引:2,自引:0,他引:2
Silva BM Casal S Andrade PB Seabra RM Oliveira MB Ferreira MA 《Journal of agricultural and food chemistry》2004,52(5):1201-1206
Twenty-one free amino acids present in several samples of quince fruit (pulp and peel) and quince jam (homemade and industrially manufactured) were analyzed by GC/FID. The analyses showed some differences between quince pulps and peels. Generally, the highest content in total free amino acids and in glycine was found in peels. As a general rule, the three major free amino acids detected in pulps were aspartic acid, asparagine, and hydroxyproline. For quince peels, usually, the three most abundant amino acids were glycine, aspartic acid, and asparagine. Similarly, for quince jams the most important free amino acids were aspartic acid, asparagine, and glycine or hydroxyproline. This study suggests that the free amino acid analysis can be useful for the evaluation of quince jam authenticity. It seems that glycine percentage can be used for the detection of quince peel addition while high alanine content can be related to pear addition. 相似文献
992.
Soils of two climosequences in Russia were investigated by 31P-NMR spectroscopy. They comprised Dystric Podzoluvisols, Haplic Greyzems, Calcic Chernozems, and Gypsic Kastanozems, which are located along temperature and precipitation gradients of the Russian Plain. Another sequence of soils included forest Humic Cambisols and Umbric Leptosols of subalpine and alpine meadows, which are formed in different climatic conditions along a climosequence of the Mt. Malaya Khatipara (northern Caucasus). The results showed that accumulation of DNA was high in the cold, wet, and acid soils (Dystric Podzoluvisol, alpine Umbric Leptosol), while phospholipids and teichoic acids mainly accumulated in the more microbially active soils. We performed a laboratory incubation experiment to test the relationship between microbial biomass P and P species identified in soil extracts. The proportions of P compounds resonating at 0.5-3.0 ppm in the NaHCO3 and H2SO4 extracts from the incubated Humic Cambisol increased. The amounts of phosphate diesters resonating at 0 ppm in the same extracts and in the subsequent NaOH extracts decreased after incubation. Based on the results of 31P-NMR spectroscopy of native soils and of the laboratory incubation experiment we concluded that signals at 0 ppm in spectra of soil alkaline extracts belong to DNA P which is mainly stabilised in soil organic matter outside microbial cells (at least in soils with relatively low microbial activity). Phospholipids-teichoic acids P extracted with 0.5 M NaHCO3 seems to be derived from soil microbial biomass, and its proportion can reflect the microbial activity in the soil. 相似文献
993.
Farmers are applying very high amounts of N fertilizer (sometimes >900 kg N/ha), commonly (NH4)2SO4, to irrigated potato (Solanum tuberosum, L.) grown on sandy textured soils in the Cappadocia region of Turkey. To obtain information on potato yield, N uptake, N fertilizer residue in the soil and the portion of N fertilizer leached below 200 cm soil depth, nine field experiments were conducted at three different locations in 1992, 1993 and 1994. The N rates used in these experiments were 0, 200, 400, 600, 800 and 1,000 kg N/ha within a completely randomized block design with three replicates. N fertilizer was applied in two equal portions; one at planting and one just before the first irrigation. Although all yield data were used to find out the marketable tuber yield, the N rate response curve and the fate of applied fertilizer N was determined only for the 400 and 1,000 kg N/ha rates. Isotope microplots were established where 15N-labelled (NH4)2SO4 was applied at 5.0 atom % and 2.5 atom % excess enrichments for the 400 kg N/ha and 1,000 kg N/ha rates, respectively. At harvest, marketable and dry tuber yield was determined for all N rates. Dry tuber and leaf plus vine yields were determined for the isotope microplots and they were analysed for the % N and 15N atom % excess. The % N derived from fertilizer and N use efficiency (%NUE) were calculated for the plant samples. The 15N-labelled residue left in 0-200 cm soil was also determined. The amount of N fertilizer leached below 200 cm soil depth was also calculated. 15N-labelled NO3- and total NO3- of the groundwater from wells were determined at different dates. Our results show that the optimum marketable tuber yield was obtained with 600 kg N/ha. Tuber N uptake was increased slightly, while leaf plus vine N uptake increased considerably when the N rate was increased from 400 to 1,000 kg N/ha. The %NUE values decreased nearly by half and the amount of N fertilizer in the 0-200 cm soil layer increased more than 3 times when the N rate was increased from 400 to 1,000 kg N/ha. Nearly half of the applied fertilizer N (45.6%) at 400 kg N/ha and more than half of the applied fertilizer N (60.8%) at 1,000 kg N/ha was still in the 0-200 cm soil layer after harvest. Four times more N fertilizer was leached below 200 cm soil depth when 1,000 kg N/ha N was applied instead of 400 kg N/ha. Our results also indicate that there is a potential contamination of groundwater due to leaching of the applied N fertilizer. 相似文献
994.
Almela C Algora S Benito V Clemente MJ Devesa V Súñer MA Vélez D Montoro R 《Journal of agricultural and food chemistry》2002,50(4):918-923
The total arsenic, inorganic arsenic, lead, cadmium, and mercury contents of 18 algae food products currently on sale in Spain were determined. The suitability of the analytical methodologies for this type of matrix was confirmed by evaluating their analytical characteristics. The concentration ranges found for each contaminant, expressed in milligrams per kilogram of dry weight, were as follows: total arsenic, 2.3-141; inorganic arsenic, 0.15-88; lead, < 0.05-1.33; cadmium, 0.03-1.9; and mercury, 0.004-0.04. There is currently no legislation in Spain regarding contaminants in algae food products, but some of the samples analyzed revealed Cd and inorganic As levels higher than those permitted by legislation in other countries. Given the high concentrations of inorganic As found in Hizikia fusiforme, a daily consumption of 1.7 g of the product would reach the Provisional Tolerable Weekly Intake recommended by the WHO for an average body weight of 68 kg. A more comprehensive study of the contents and toxicological implications of the inorganic As present in the algae food products currently sold in Spain may be necessary, which might then be the basis for the introduction of specific sales restrictions. 相似文献
995.
López-Valdivia LM Fernández MD Obrador A Alvarez JM 《Journal of agricultural and food chemistry》2002,50(6):1455-1460
Experiments under laboratory and greenhouse conditions were conducted to study the response of maize (Zea mays L.) to Zn fertilizer applications (Zn-phenolate, Zn-EDDHA, Zn-EDTA, Zn-lignosulfonate, Zn-polyflavonoid, and Zn-heptagluconate) in an Aquic Haploxeralf soil. The application of Zn complexes significantly increased Zn uptake by the plant compared with that in the control soil. The highest enhancements were obtained in soil treated with Zn-EDTA, Zn-lignosulfonate, and Zn-EDDHA. The highest percentages of Zn taken up by the plants occurred when 20 mg x kg(-1) Zn was applied as Zn-EDTA fertilizer and 10 mg x kg(-1) as Zn-lignosulfonate fertilizer. In the greenhouse experiment, Zn speciation in soil after harvesting showed that almost all Zn was found in the residual fraction followed by metal in the water-soluble plus exchangeable fraction and metal bound to organic matter. The most effective fertilizers maintaining Zn in the most labile fractions were Zn-phenolate, Zn-EDTA, and Zn-lignosulfonate. Conversely, in the incubation experiment, only a small percentage of Zn was found in the water-soluble plus exchangeable fraction and no differences in the Zn distribution were observed between the different fertilizer treatments. The micronutrient content in maize was positively correlated with the water-soluble plus exchangeable Zn as well as with the available Zn determined by the diethylenetriaminepentaacetic acid and Mehlich-3 methods, in the greenhouse experiment. Results of this study showed that the incubation experiment in acidic soil is not a suitable tool to establish the different effectiveness of Zn chelates in plants. 相似文献
996.
Fundira M Blom M Pretorius IS van Rensburg P 《Journal of agricultural and food chemistry》2002,50(6):1535-1542
Juice of the Sclerocarya birrea subsp. caffra (marula) fruit was fermented by indigenous microflora and different commercial Saccharomyces cerevisiae yeast strains at different temperatures, namely, 15 and 30 degrees C. Volatile acids, esters, and higher alcohols were quantified in the wine and distillates, and the results were interpreted using a multivariate analysis of variance and an average linkage cluster analysis. Significant differences between 15 and 30 degrees C and also among yeasts with respect to volatile compounds were observed. Yeast strains VIN7 and FC consistently produced wines and final distillates significantly different from the other strains. A panel of tasters and marula and brandy producers was asked to select wines and distillates that had an acceptable and typical marula "nose". They were also asked to detect the differences among wines and distillates fermented with the same yeast strain at different temperatures. 相似文献
997.
Botanical origin and chemical composition of Brazilian propolis 总被引:11,自引:0,他引:11
Brazilian propolis has been classified into 12 groups based on physicochemical characteristics: five in the southern Brazil group (group 3), one in the southeastern Brazil group (group 12), and six in the northeastern Brazil group (group 6). The plant origins of these groups were investigated using reversed-phase high-performance thin-layer chromatography (RPHPTLC), reversed-phase high-performance liquid chromatography (RPHPLC), and gas chromatography-mass spectrometry (GC-MS). It was concluded that the origins of propolis group 3, group 6, and group 12 are resins of the poplar tree, Hyptis divaricata, and Baccharis dracunculifolia, respectively. 相似文献
998.
The sulfonylurea herbicide prosulfuron, 1-(4-methoxy-6-methyltriazin-2-yl)-3-[2-(3,3,3-trifluoropropyl)phenylsulfonyl]urea, is used for the selective control of broadleaf weeds in corn, sorghum, and cereal grains. To investigate its fate in soils, this study examined the effects of soil pH and water content on the rates of dissipation processes and the products formed under aerobic conditions. Radiometry and chromatography analyses were used to quantify the degradation products and bound residues formed in incubations of 10 different soils. The pH-dependent hydrolysis of the sulfonylurea bridge to form phenyl sulfonamide was the primary transformation process. Significant microbial degradation of prosulfuron occurred in 2 of the 10 soils, yielding (14)CO(2) and desmethyl prosulfuron among the major products. The time required for 50% dissipation of the herbicide (DT(50)) was determined for each soil and water content treatment. At equivalent water contents, prosulfuron DT(50) values were positively correlated with soil pH (P < 0.0001), varying from 6.5 days at pH 5.4 to 122.9 days at pH 7.9. Soil pH and water content strongly influence the fate of sulfonylurea herbicides in agricultural fields. Differences in the effect of soil water content on dissipation kinetics in a comparison of two soils were attributed to differences in soil pH, texture, and the ability of indigenous microorganisms to transform the herbicide. 相似文献
999.
1000.
Absorption of phenolic acids in humans after coffee consumption 总被引:10,自引:0,他引:10
Nardini M Cirillo E Natella F Scaccini C 《Journal of agricultural and food chemistry》2002,50(20):5735-5741
Despite extensive literature describing the biological effects of polyphenols, little is known about their absorption from diet, one major unresolved point consisting of the absorption of the bound forms of polyphenols. In this view, in the present work we studied the absorption in humans of phenolic acids from coffee, a common beverage particularly rich in bound phenolic acids, such as caffeic acid, ferulic acid, and p-coumaric acid. Coffee brew was analyzed for free and total (free + bound) phenolic acids. Chlorogenic acid (5'-caffeoylquinic acid), a bound form of caffeic acid, was present in coffee at high levels, while free phenolic acids were undetectable. After alkaline hydrolysis, which released bound phenolic acids, ferulic acid, p-coumaric acid, and high levels of caffeic acid were detected. Plasma samples were collected before and 1 and 2 h after coffee administration and analyzed for free and total phenolic acid content. Two different procedures were applied to release bound phenolic acids in plasma: beta-glucuronidase treatment and alkaline hydrolysis. Coffee administration resulted in increased total plasma caffeic acid concentration, with an absorption peak at 1 h. Caffeic acid was the only phenolic acid found in plasma samples after coffee administration, while chlorogenic acid was undetectable. Most of caffeic acid was present in plasma in bound form, mainly in the glucuronate/sulfate forms. Due to the absence of free caffeic acid in coffee, plasma caffeic acid is likely to be derived from hydrolysis of chlorogenic acid in the gastrointestinal tract. 相似文献