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41.
Thiago Luiz Da Mata Monique Inês SegerenAline Segeren Fonseca Carlos Augusto Colombo 《Scientia Horticulturae》2009
Genetic diversity was evaluated by RAPD markers and morpho-agronomic characters for a total of 42 accessions of Barberton daisy (Gerbera jamesonii) consisting of 29 commercial and 13 wild accessions. A total of 74 polymorphic bands were obtained employing a set of 12 primer pairs. The average genetic similarity coefficient for the 42 accessions, evaluated by Jaccard index was 0.55 ranging from 0.28 to 1.00. The genetic structure found among Barberton daisy accessions was evaluated by hierarchic classification analyses and UPGMA modeling, revealing six clusters of genotypes where two of them include the wild accessions and the remaining four including commercial material, except for wild genotype number 9. Shannon (H′) index was calculated using the molecular markers to investigate the genetic variation among the Gerbera accessions and showed higher values for the commercial cluster in comparison to the values obtained for the individuals from the non-commercial cluster, namely 0.34 versus 0.27, respectively. Therefore, both calculated indices (Jaccard and Shannon) indicated the presence of higher genetic variation among commercial accessions in comparison to the cluster representing non-commercial accessions, suggesting that genetic breeding programs may focus on commercial accessions to recombine interesting genotypes with commercially important and marketing-desired characteristics. 相似文献
42.
Igo G. Guimarães Luiz E. Pezzato Margarida M. Barros Leonardo Tachibana 《Journal of the World Aquaculture Society》2008,39(6):781-789
Apparent digestibility coefficients (ADCs) provide estimates of nutrient availability in feedstuffs and can be used as a tool for selecting ingredients to compound cost‐effective diets for fish. Extrusion process is one factor that could influence ADC values. Thus, a digestibility trial was undertaken to evaluate ADC of protein, energy, and dry matter, and apparent amino acid availability (AAAA) in five cereal grain products/by‐products (corn, wheat middlings, broken rice, rice bran, and sorghum) in compounded diets for Nile tilapia. Chromic oxide was used as an external inert marker. The digestibility values of energy and dry matter in ingredients, in decreasing order, were highest for broken rice (95.34 and 96.45%), sorghum (82.37 and 87.29%), corn (67.34 and 82.21%), rice bran (57.58 and 55.59%), and wheat middlings (48.94 and 45.88%). Protein digestibility value was higher for corn (72.86%) and the lowest value was observed for sorghum (56.77%), while broken rice, wheat middlings, and rice bran presented values ranging from 63.01 to 66.88%. AAAA values were higher for corn (70.05%) and the lowest methionine and cystine availability values were recorded for wheat middlings and broken rice, respectively. Broken rice appears as one of the most indicated ingredient for using in Nile tilapia diets because of its highest values of nutrient digestibility when compared to the other energetic sources. The use of this information should aid more precise and economic diet formulation for Nile tilapia. 相似文献
43.
Itacir P. Ubert Cristiano M. Zimmer Kelly Pellizzaro Luiz C. Federizzi Itamar Cristiano Nava 《Euphytica》2017,213(2):41
Avena sativa L. subsp. nudisativa has the ability to produce naked grains. Genetic studies on the naked trait of oat began over a century ago, but the genetic and molecular factors associated with the expression of this trait have not been fully clarified. The objectives of this study were to evaluate the naked trait in two oat populations of recombinant inbred lines (RILs), to determine the number of genes, to estimate the heritability, and to map genomic regions associated with the naked trait in hexaploid oat. Parental lines and RILs of each population were screened for the naked trait from plants grown in the field over a 2 year period. Based on the phenotypic data, the oat RILs were classed as naked, partially naked, partially hulled and hulled. In both populations and years, a great number of RILs showed variable expressivity for the naked trait. The genetic analysis indicated the action of a major gene (N1) with the action of modifying genes controlling the formation of naked grains. The results of the estimate of heritability show that environmental conditions do not have a great influence in determining the naked trait. The quantitative trait loci analysis detected a genomic region with a large effect on the naked trait that explained more than 50% of the phenotypic variation. Further studies are needed to validate the use of these molecular markers to assist breeding programs to select high quality and stable naked oat cultivars. 相似文献
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Fernández PL Pablos F Martín MJ González AG 《Journal of agricultural and food chemistry》2002,50(7):1833-1839
47.
Escribano J Gandía-Herrero F Caballero N Pedreño MA 《Journal of agricultural and food chemistry》2002,50(21):6123-6129
The two enzymes involved in enzymatic browning reactions, polyphenol oxidase (PPO) and peroxidase (PO), have been partially purified and extracted from different fractions of beet root. PPO is mainly located in the membrane fraction, and it was also found in the soluble fraction. In both cases PPO was in its latent state. However, PO activity was higher in the soluble fraction than in the membrane fraction. Nevertheless, the highest values of specific activity for PO were obtained from the solubilized enzyme from acetone powders. Under native isoelectric focusing (IEF), several PPO isoenzymes were present in the pH range of 4.8-5.8. All of these isoenzymes shared a single band with a similar apparent mass under sodium dodecyl sulfate-polyacrylamide gel electrophoresis. PO was also analyzed by IEF, showing a complex isoenzyme pattern in all fractions. The characteristic basic PO isoenzyme of high pI found in both the soluble fraction and the solubilized enzyme from acetone powders was not detected in the membrane fraction. The kinetic characterization of PPO and PO from all fractions was carried out. 相似文献
48.
Pakdeechanuan P Intarapichet KO Fernando LN Grün IU 《Journal of agricultural and food chemistry》2005,53(4):923-927
Rhodium heterogeneous catalyst was used to catalyze isomerization of linoleic acid in soybean oil to conjugated linoleic acid (CLA). A central composite rotatable design with five levels of three variables, namely, reaction temperature, stirring speed, and reaction time, was used to determine the maximum CLA yield. The formation of CLA during isomerization was greatly dependent on the reaction temperature and time. The CLA content of soybean oil increased from 0.63 to 202.42 mg/g oil when isomerization was done at 200 degrees C, with a stirring speed of 200 rpm for 49 min. Analysis of triacylglycerol positions showed that linoleic acid at any position in a triacylglyceride could possibly be isomerized to CLA. 相似文献
49.
Arroyo FT Moreno J Daza P Boianova L Romero F 《Journal of agricultural and food chemistry》2007,55(14):5701-5707
Eight volatile products characterizing strawberry aroma, which is generated from the oxidative degradation of linoleic and linolenic acids by a lipoxygenase (LOX) pathway, were examined because of their antifungal activity against Colletotrichum acutatum, one of the causal agents of strawberry anthracnose. In this study, the effects of aldehydes, alcohols, and esters on mycelial growth and conidia development were evaluated. (E)-Hex-2-enal was found to be the best inhibitor of mycelial growth [MID (minimum inhibitory doses)=33.65 microL L(-1)] and of spore germination (MID=6.76 microL L(-1)), while hexyl acetate was the least effective of all volatile compounds tested (MID=6441.89 microL L(-1) for mycelial growth and MID=1351.35 microL L(-1) for spore germination). Furthermore, the antifungal activity of (E)-hex-2-enal on susceptibility of strawberry fruits to C. acutatum was also confirmed. The presence of these molecules in jars containing strawberry fruits inoculated with a suspension of spores inhibited the fungus growth and prevented the appearance of symptoms. Moreover, a study of the effects of (E)-hex-2-enal on conidial cells of C. acutatum was evaluated by transmission electron microscopy. This volatile compound altered the structures of the cell wall and plasma membrane, causing disorganization and lysis of organelles and, eventually, cell death. 相似文献