全文获取类型
收费全文 | 1795篇 |
免费 | 107篇 |
专业分类
林业 | 159篇 |
农学 | 57篇 |
基础科学 | 31篇 |
418篇 | |
综合类 | 82篇 |
农作物 | 123篇 |
水产渔业 | 231篇 |
畜牧兽医 | 635篇 |
园艺 | 35篇 |
植物保护 | 131篇 |
出版年
2024年 | 2篇 |
2023年 | 9篇 |
2022年 | 51篇 |
2021年 | 72篇 |
2020年 | 60篇 |
2019年 | 74篇 |
2018年 | 72篇 |
2017年 | 67篇 |
2016年 | 72篇 |
2015年 | 69篇 |
2014年 | 95篇 |
2013年 | 139篇 |
2012年 | 137篇 |
2011年 | 164篇 |
2010年 | 78篇 |
2009年 | 91篇 |
2008年 | 87篇 |
2007年 | 105篇 |
2006年 | 91篇 |
2005年 | 66篇 |
2004年 | 67篇 |
2003年 | 62篇 |
2002年 | 43篇 |
2001年 | 9篇 |
2000年 | 11篇 |
1999年 | 17篇 |
1998年 | 12篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 4篇 |
1992年 | 8篇 |
1991年 | 2篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1976年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1969年 | 2篇 |
1967年 | 1篇 |
1963年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有1902条查询结果,搜索用时 31 毫秒
61.
Lopera L Moro PL Chavez A Montes G Gonzales A Gilman RH 《Veterinary parasitology》2003,117(1-2):37-42
One hundred and six dogs (61 males and 45 females) were examined for Echinococcus granulosus infection in a farming cooperative in the central highlands of Peru during November 1998. Canine echinococcosis was diagnosed using direct microscopic examinations of purged feces following arecoline purging and a coproantigen-detection enzyme-linked immunosorbent assay (ELISA) for E. granulosus. Mean age was 2 years with a range of 3 months to 9 years. The overall prevalence of canine echinococcosis using the ELISA test was 79% (84/106). Seventy-four dogs were successfully purged with arecoline. The frequency of canine echinococcosis was 82 (61/74) and 34% (25/74) by the coproantigen ELISA test and arecoline purging, respectively. The sensitivity and specificity of the coproantigen ELISA test was 88 and 95%, respectively. We found this assay to be especially advantageous in remote geographical areas. In future control programs against echinococcosis in Peru and other areas where E. granulosus is endemic the coproantigen ELISA should be used for the surveillance of the dog population. 相似文献
62.
In this study, we determined the occurrence of the tsh gene among 305 Escherichia coli isolates from chickens by means of the polymerase chain reaction and agglutination of chicken erythrocytes; 200 of those isolates were obtained from chickens with colisepticemia, 52 isolates were from lesions of cellulitis, and 53 were from feces of normal chickens. The tsh gene was found in 79 (39.5%) isolates from colisepticemia, in 10 (19%) cellulitis-derived E. coli isolates, and in two (3.8%) fecal isolates. Among the tsh+ strains, 68 (86%) isolates from colisepticemia and nine (90%) from cellulitis agglutinated chicken erythrocytes in the presence of mannose, after growing the strains on colonization factor antigen agar plates at 26 C, which confirms a correlation between mannose-resistant hemagglutination and expression of hemagglutinin Tsh. These results show, for the first time, the presence of the gene tsh in cellulitis-derived E. coli isolates; the high frequency of this gene among avian pathogenic E. coli isolates in Brazil indicates that its putative role as a virulence factor should be studied more thoroughly. 相似文献
63.
Guarga JL Moreno J Lucientes J Gracia MJ Peribáñez MA Castillo JA 《Veterinary immunology and immunopathology》2002,88(1-2):13-20
The efficacy of specific immunochemotherapy against Leishmania infantum infection in dog was studied. The effects on transmission of the disease, as well as the cellular and humoral immune response were examined. The treated animals showed a significant reduction in the infection rates that were detected in Phlebotomus perniciosus females fed on the dog. The humoral immune response, assayed with an indirect immunofluorescence antibody test (IFAT), did not show significant variations under the influence of the therapy. The characterisation of the peripheral blood mononuclear cells (PBMC) using flow cytometry indicated a significant increase in the proportion of T lymphocytes, especially of CD4/TcR(alpha)(beta)(+) and CD4/CD45RA(+) cells, without showing evidence for modifications in the other leukocyte subsets. Cellular lymphoproliferation studies indicated a lack of a specific response to soluble leishmanial antigen (SLA), but the non-specific lymphoproliferative capacity assayed with phytohemagglutinin (PHA) was maintained. 相似文献
64.
Samartino LE 《Veterinary microbiology》2002,90(1-4):71-80
Brucellosis has been recognized in Argentina since the 19th century. Several studies demonstrated the presence of the disease in most of the domestic species. Actually, the estimate of prevalence is that between 10 and 13% of the farm animals are infected with bovine brucellosis with an individual rate of 4–5%. The annual economical losses have been estimated at US$ 60,000,000. The control of bovine brucellosis began in 1932 and successive resolutions have been issued since then. The current resolution indicates that B. abortus S19 is mandatory in female calves between 3 and 8 months of age. The vaccine strain B. abortus RB51 was provisionally approved but only for cattle older than 10 months of age. The brucellosis control program consists principally of test and slaughter. This methodology has been successful mainly in the dairy farms that have the incentive due to increased pricing because of obtaining a low prevalence of the disease. Brucellosis has been found in porcine, caprine, ovine and canine species. All Brucella species have been found in the country. Human brucellosis is an important disease and a national coordinated diagnostic net has been formed to better control the disease in man. 相似文献
65.
Excessive growth and consequent deformity of hooves is a frequent disorder in some purebred pig populations. A test to detect possible genetic determinism related with this phenomenon was performed using the Bayes Factor (BF). Data were available for females from three purebred selection lines: Landrace (561 records), Pietrain (183) and Large White (225). Animals were scored in four categories, according to the overall growth rate of their hooves. A Bayesian analysis was performed separately for each line using a threshold model with a probit approach, and Bayes Factors between models with and without additive genetic effects were computed. Results from the three lines showed that models exhibiting genetic variability were much more probable than those that did not include a genetic component, with BF values of 312, 35 and 40 (and posterior probabilities of 0.99, 0.97 and 0.98), respectively, for the Landrace, Pietrain and Large White lines. Monte Carlo estimates of posterior means of heritabilities were medium to high (0.25, 0.41 and 0.38, respectively), and the highest posterior density region for heritability at 99% did not include zero in any of the three lines. These results allow us to conclude that genetic determinism has an important influence upon the rate of hoof growth in the pig. A potential genetic response can be achieved in the populations analysed, but further studies are needed to determine the genetic architecture of hoof growth disorders in pigs. 相似文献
66.
Christopher J Bonar Ernesto O Boede Manuel García Hartmann Joanne Lowenstein-Whaley Esmeralda Mujica-Jorquera Scott V Parish James V Parish Michael M Garner Cynthia K Stadler 《Journal of zoo and wildlife medicine》2007,38(2):177-191
River dolphins are especially susceptible to negative human impacts. For their conservation, attempts of relocation or procreation ex situ may become important in the future to avoid their extinction. Additional knowledge and medical experiences of river dolphin management in captivity may aid such conservation efforts. The medical records and necropsy and histopathology reports on 123 captive Amazon River dolphins (Inia geoffrensis) were re-viewed. Of these 123 animals, 105 were necropsied and 70 necropsies were supported with histopathology. Eighteen animals were not necropsied. Among wild-born animals, mortality was highest in the first 2 mo immediately postcapture and transport, accounting for 32 of 123 deaths. Pneumonia and skin lesions (cutaneous and subcutaneous ulcerations and abscesses) were the most common findings, found in 44 of 105 (42%) and 38 of 105 (36%) of gross diagnoses, respectively. At least 10 of 44 cases of pneumonia diagnosed grossly included a verminous component. Cachexia, from a variety of causes, was a major gross finding in 21 animals. Fifteen animals had histologic evidence of significant renal pathology, and this was the primary cause of death in 13 cases. Hepatic pathology was found in 18 cases, and bacterial sepsis was confirmed via histology in 16 cases. Based on these findings, it may be concluded that keys to successful maintenance of this species include 1) prophylactic anthelminthic and antibiotic therapy immediately post-capture; 2) maintenance of animals in larger enclosures than in past attempts, in compatible groups, and in facilities capable of separating aggressive animals; 3) maintenance in microbiologically hygienic water quality at all times; and 4) a proactive program of preventive medicine during the immediate postcapture, quarantine, and maintenance period of captivity. 相似文献
67.
José Luis Palomo María M. López Pablo García-Benavides Encarna Velázquez Eustoquio Martínez-Molina 《European journal of plant pathology / European Foundation for Plant Pathology》2006,115(4):443-451
API 50CH and API ZYM systems were used to characterize fifty-three strains of Clavibacter michiganensis subsp. sepedonicus from different geographic locations and several reference strains of the same and different species, including other potato pathogens. Clavibacter michiganensis subsp. sepedonicus strains displayed a high level of homogeneity, both in carbohydrate utilization and in enzymatic activity. Using API 50CH and API ZYM it was possible to differentiate C. michiganensis subsp. sepedonicus strains from the remaining taxa analysed in this study, which included representative strains of the other subspecies of C. michiganensis as well as other bacterial pathogens affecting potatoes. Therefore, these systems could be used as an effective method to characterize C. michiganensis subsp. sepedonicus. Such a procedure would constitute an alternative system to the conventional nutritional and physiological identification tests currently included in the official methods employed in the European Union to detect and identify this bacterium. The results obtained with the API systems agreed with the current taxonomic classification of C. michiganensis, clearly separating sepedonicus from the other subspecies belonging to this species. 相似文献
68.
(接《新饲料》第10期第14页)2针对食品安全进行饲料的营养调控2.1霉菌毒素的控制霉菌毒素是最重要的饲料污染物之一,并且会给动物以及公众健康带来严重影响。霉菌毒素有多种危 相似文献
69.
Lima Pedro Paulo Arcanjo Narciso Luis Gustavo Alcindo Jefferson Filgueira Deschk Maurício Ciarlini Paulo Cesar dos Santos Paulo Sérgio Patto de Almeida Breno Fernando Martins 《Veterinary research communications》2022,46(1):27-35
Veterinary Research Communications - Propofol is a widely used drug in veterinary medicine to induce anesthesia; as well as the chosen compound for protocols of intravenous anesthesia. The present... 相似文献
70.
Potential errors in measurement of nonuniform sap flow using heat dissipation probes 总被引:1,自引:0,他引:1
The empirical calibration of Granier-type heat dissipation sap flow probes that relate temperature difference (DeltaT) to sap velocity (v) was reevaluated in stems of three tropical tree species. The original calibration was confirmed when the entire heated probe was in contact with conducting xylem, but mean v was underestimated when part of the probe was in contact with nonconducting xylem or bark. Analysis of the effects of nonuniform sap velocity profiles on heat dissipation estimates showed that errors increased as v and the proportion of the probe in nonconducting wood increased. If half of a 20-mm probe is in sapwood with a v of 0.15 mm s(-1) and the other half is in nonconducting wood, then mean v for the whole probe can be underestimated by as much as 50%. A correction was developed that can be used if the proportion of the probe in nonconducting wood is known. Even with the entire heated probe in contact with conducting xylem, v would be underestimated when radial velocity gradients are present. In this case, the error would be smaller except when velocity gradients are very steep, as can occur in species with ring-porous wood anatomy. Errors occur because the relationship between DeltaT and v is nonlinear. Mean DeltaT along the probe is therefore not a measure of mean v, and users of heat dissipation probes should not assume that v is integrated along the length of the probe. The same type of error can occur when DeltaT is averaged through time while v is changing, but the error is small unless there are sudden, step changes between zero and high sap velocity. It is recommended that relatively short probes (20 mm or less) be used and that probes longer than the depth of conducting sapwood be avoided. Multiple probes inserted to a range of depths should be used in situations where steep gradients in v are expected. If these conditions are met, heat dissipation probes remain useful and widely applicable for measuring sap flow in woody stems. 相似文献