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11.
12.
Positive PCR alone should not be considered sufficient to establish Dirofilaria repens as the cause of subcutaneous nodular lesions in the absence of a clear cytologic picture 下载免费PDF全文
13.
Thibaut Olivier Vaidevutis Šveikauskas Elisabeth Demonty Kris De Jonghe Pascal Gentit Mojca Viršček-Marn Sabine Grausgruber-Gröger Sébastien Morio Francesco Faggioli Michèle Visage Frédéric Fauche Maria Gusina Marta Luigi Helena Lasner Irena Mavrič-Pleško 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(3):645-654
14.
Vitis vinifera L. cv. Pinot blanc was grown in both pot and commercial vineyard under lime‐stress conditions. Dark green to very chlorotic young leaves were sampled in order to analyze chlorophyll and iron (Fe) concentration and content, area, fresh and dry weight, specific weight per area. Iron content per individual leaf was a good parameter to separate dark green leaves from chlorotic ones in both trials, while Fe concentration on DW basis was not. Chlorosis occurrence always decreased leaf dry weight and specific weight per area. 相似文献
15.
利用智能LCR测量仪和平板电极系统,用无损检测方法测定了桃子在储藏过程中电特性的变化。结果表明:在12Hz~100kHz的频段内,桃子的最佳测试频段为15kHz以下,此时桃子随着储藏时间的增加,等效阻抗增大而相对介电常数和介质损耗因数减小;当桃子开始腐烂时,电特性数值出现了一个大的反复 相似文献
16.
Alfredo Di Filippo Alfredo Alessandrini Franco Biondi Silvia Blasi Luigi Portoghesi Gianluca Piovesan 《Annals of Forest Science》2010,67(7):706-706
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• We combined stem volume increment analysis with dendroecological tools to address two unresolved issues concerning oak dieback
in Mediterranean areas: early detection of changes in stand growth, and identification of mechanisms for observed growth declines. 相似文献
17.
de Campos Mateus Ribeiro Béarez Philippe Amiens-Desneux Edwige Ponti Luigi Gutierrez Andrew Paul Biondi Antonio Adiga Abhijin Desneux Nicolas 《Journal of pest science》2021,94(3):829-842
Journal of Pest Science - The South American tomato pinworm, Tuta absoluta, (SATP) is now a devastating pest worldwide of crops in the family Solanaceae. Most prior studies of SATP’s thermal... 相似文献
18.
Mark K. Asplen Gianfranco Anfora Antonio Biondi Deuk-Soo Choi Dong Chu Kent M. Daane Patricia Gibert Andrew P. Gutierrez Kim A. Hoelmer William D. Hutchison Rufus Isaacs Zhi-Lin Jiang Zsolt Kárpáti Masahito T. Kimura Marta Pascual Christopher R. Philips Christophe Plantamp Luigi Ponti Gábor Vétek Heidrun Vogt Vaughn M. Walton Yi Yu Lucia Zappalà Nicolas Desneux 《Journal of pest science》2015,88(3):469-494
19.
Estimating evapotranspiration in the Padova Botanical Garden 总被引:1,自引:0,他引:1
In 1996, intensive building operations near the botanical garden of Padova, the oldest botanical garden in the world, altered a long-established equilibrium between groundwater and plants and threatened the lives of some of them. To avoid water stress, an advanced irrigation system was installed. For design purposes, better knowledge of the water cycle and the monthly average evapotranspiration (ET) in the area was needed. Due to the complex canopy stand of the site, ET was estimated using the water balance method, integrating mathematical models with the Arc/Info Geographical Information System. The water balance was estimated in 1997 and 1998, and results were used to derive an empirical mean crop coefficient of the botanical garden, to simulate the long-term water requirements using the product of reference ET and the apparent crop coefficient to estimate ET from the garden. Two types of hydrological behaviour were identified: one in the central area of the garden, where reduced ground cover diminishes ET and increases runoff and percolation. In the external area, the ET was higher because of the presence of many trees. The empirical mean monthly crop coefficient ranged between 0.56 and 0.83, indicating that ET in the entire area is always less than grass reference ET. 相似文献
20.
Luigi Pane Elisabetta Giacco Christian Corrà Giuliano Greco Gian Luigi Mariottini Franco Varisco Marco Faimali 《Journal of Soils and Sediments》2008,8(2):74-79
Background, Aim and Scope The toxicity of contaminated sediments should be evaluated considering the direct exposure of laboratory organisms to whole
sediments and the indirect exposure to elutriates or extracts (Tay et al. 1992, Byrne and Halloran 1999, Nendza 2002). The
alga Dunaliella tertiolecta is indicated for the use in toxicity bioassays because it is highly sensitive to several xenobiotics. Harpacticoid copepods
have been already used for toxicity testing and Tigriopus fulvus is a promising Mediterranean target-species in ecotoxicology (Todaro et al. 2001, Faraponova et al. 2003, Pane et al. 2005a).
In this study, the toxicity of sediments collected in harbour sites of the Northeastern Adriatic Sea was evaluated by growth
inhibition test with free living and alginate-immobilized Dunaliella tertiolecta and acute toxicity test with nauplii and adult Tigriopus fulvus with the aim of pointing out the importance to utilize model organisms from different trophic levels in sediment ecotoxicology.
Methodology Elutriates and whole sediments were tested on free living and immobilized (Pane et al. 1998) algal cells, and on laboratory
reared copepods. Free-living D. tertiolecta were exposed to diluted elutriates in a static, multi-well plate system. Naalginate immobilized D. tertiolecta were placed in polystyrene inserts fitted with polyester mesh bottoms and exposed to a thin layer (2 mm) of whole sediments
in multi-well plates (EPS 1992, Pane and Bertino 1999). Toxicity tests with copepods were carried out on Tigriopus fulvus nauplii (elutriates) and adults (whole sediments and elutriates). Same-aged nauplii useful for toxicity tests were obtained
by egg sac detaching and consequent hatching stimulation (Pane et al. 2006). Newborn nauplii (I–II stage) were exposed to
elutriates in multi-well plates provided with polystyrene inserts. Adult T. fulvus maintained in polystyrene inserts fitted with polyester mesh bottoms were placed in contact with a thin layer (2 mm) of whole
sediment placed on multi-well plate bottoms. All end-points were evaluated after 96 h.
Results In general, the effects increased with the increasing of elutriate concentration up to 50%; the stimulation or inhibition
of algal growth was statistically significant in comparison to the control. The inhibiting elutriates induced EC50 variations of algal growth ranging from 66.9% to 74.3%. The mortality of T. fulvus nauplii was always < 25% after treatment with 100% elutriates and < 10% after treatment with 50% dilution; no effect was
shown up with 25% dilution; therefore LC50 was not calculable. The effect of elutriates was negligible on adult copepods and LC50 values were never calculable; percent mortality always resulted in < 10% after treatment with whole sediments.
Discussion Both experimental systems gave substantially similar results after exposition to whole sediments and elutriates. During the
experiment with algal cells, the immobilization in Na-alginate and the employment of inserts which allowed the contact of
organisms with sediments and their easy counting were particularly useful. Likewise, the employment of inserts of adequate
mesh size in the tests with copepods allowed the contact of organisms with the sediment and made organism handling and counting
easy, as well as the evaluation of mortality. The methodology here described and the utilization of the proposed test-species
could have an importance also considering that the current trend in ecotoxicological research is towards finding the most
appropriate organism for specific areas of concern by using indigenous species (Mariani et al. 2006) and towards the major
significance of chronic and reproductive end-points.
Conclusions Based on the above results, it can be stated that the bioassay with Dunaliella tertiolecta could be a good estimation tool for the ecotoxicological assessment of marine sediments. The immobilization of algae in Na-alginate
was seen to be useful to evaluate the toxicity of whole sediments; the employment of polystyrene inserts allowed an improvement
of the procedures. T. fulvus nauplii and adults, as other harpacticoids such as Tigriopus japonicus (Yoon et al. 2006), satisfy the basic criteria for the employment of a standard species in marine bioassays. To date only
pelagic Acartia tonsa are utilized in the standardized procedure to evaluate the risk assessment of chemicals or wastewaters (ISO 1999). As, on
the contrary, the exposure of copepods to solid-phase contaminants it is not yet standardized, the employment of polystyrene
inserts improved the procedures for T. fulvus too. So, the rapidity and the possibility to solve practical problems could be the main attractive features of this technique
(Pane et al. 2005a) when applied to whole sediments.
Recommendations and Perspectives The methodology here developed being also applicable to long term and reproduction tests should be recommended because it
provides relevant information in comparison with other frequently applied, standardized biotests with crustaceans (ISO 1999).
The procedure has been shown to be easily applicable to selected marine organisms.
ESS-Submission Editor: Prof. Dr. Henner Hollert (henner.hollert@bio5.rwth-aachen.de) 相似文献
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