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51.
Alessandra Lagomarsino Anna Benedetti Sara Marinari Letizia Pompili M. Cristina Moscatelli Pier Paolo Roggero Roberto Lai Luigi Ledda Stefano Grego 《Biology and Fertility of Soils》2011,47(3):283-291
Five soils characterised by different agro-forest managements, typical of Mediterranean environment and with increasing human
impact were chosen in Sardinia (Italy): two vineyards with different management systems, a rotation hay crop-pasture and a
forest (Quercus suber L.). The study aimed to investigate the relationships between C storage and microbial functionality in soil under different
managements. Pools of total organic C and microbial biomass C were determined, as well as the loss of organic C due to microbial
respiration (basal and cumulative) and several microbial indices (metabolic, mineralization, and microbial quotient) as indicators
of the microbial efficiency in the use of energy and the degree of substrate limitation for soil microbes. Enzymes were chosen
on their relevance in the C (β-cellobiohydrolase, N-acetyl-β-glucosaminidase, β-glucosidase, α-glucosidase), N (leucine aminopeptidase), S (arylsulphatase) and P (acid phosphatase)
cycling and were used as indicators of functional diversity in soil. Organic C pools and enzyme activities on average increased
noticeably in soils with a lower human impact showing the highest values in forest and the lowest in the vineyards, following
the trend of organic matter availability. The trend in functional diversity reflected the increase of microbial pool and organic
C availability: the vineyards showed a lower Shannon’s diversity index, whilst pasture and forest sites reached the maximum
levels of functional diversity. These soils showed an increase of microbial efficiency in the use of available resources and
the decrease of substrate limitation for soil microbes. 相似文献
52.
Towards an improved and more flexible representation of water stress in coupled photosynthesis-stomatal conductance models 总被引:1,自引:0,他引:1
Gregorio Egea Anne Verhoef Pier Luigi Vidale 《Agricultural and Forest Meteorology》2011,151(10):1370-1384
Coupled photosynthesis-stomatal conductance (A-gs) models are commonly used in ecosystem models to represent the exchange rate of CO2 and H2O between vegetation and the atmosphere. The ways these models account for water stress differ greatly among modelling schemes. This study provides insight into the impact of contrasting model configurations of water stress on the simulated leaf-level values of net photosynthesis (A), stomatal conductance (gs), the functional relationship among them and their ratio, the intrinsic water use efficiency (A/gs), as soil dries. A simple, yet versatile, normalized soil moisture dependent function was used to account for the effects of water stress on gs, on mesophyll conductance (gm) and on the biochemical capacity. Model output was compared to leaf-level values obtained from the literature. The sensitivity analyses emphasized the necessity to combine both stomatal and non-stomatal limitations of A in coupled A-gs models to accurately capture the observed functional relationships A vs. gs and A/gsvs. gs in response to drought. Accounting for water stress in coupled A-gs models by imposing either stomatal or biochemical limitations of A, as commonly practiced in most ecosystem models, failed to reproduce the observed functional relationship between key leaf gas exchange attributes. A quantitative limitation analysis revealed that the general pattern of C3 photosynthetic response to water stress may be well represented in coupled A-gs models by imposing the highest limitation strength to gm, then to gs and finally to the biochemical capacity. 相似文献
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54.
Vitis vinifera L. cv. Pinot blanc was grown in both pot and commercial vineyard under lime‐stress conditions. Dark green to very chlorotic young leaves were sampled in order to analyze chlorophyll and iron (Fe) concentration and content, area, fresh and dry weight, specific weight per area. Iron content per individual leaf was a good parameter to separate dark green leaves from chlorotic ones in both trials, while Fe concentration on DW basis was not. Chlorosis occurrence always decreased leaf dry weight and specific weight per area. 相似文献
55.
Resistance to blackleg and tuber soft rot in sexual and somatic interspecific hybrids with different genetic background 总被引:1,自引:0,他引:1
Domenico Carputo Teodoro Cardi Mario Speggiorin Astolfo Zoina Luigi Frusciante 《American Journal of Potato Research》1997,74(3):161-172
Diploid interspecific hybrids from crosses betweenSolanum tuberosum haploids andS. canasense, S. multidissectum, andS. tarijense, along with tetraploid and hexaploid somatic hybrids S.tuberosum (+)S. commersonii, were screened for resistance to blackleg and tuber soft rot byErwinia carotovora subsp.atroseptica andE. carotovora subsp.carotovora. Among the diploid hybrids, those involving clone mlt la of S.multidissectum and tar 2b ofS. tarijense yielded the highest number of genotypes resistant to both blackleg and tuber soft rot. By contrast, all the hybrids involving clone tar 1lb ofS. tarijense were susceptible to both bacterial diseases. As far as resistance of somatic hybrids is concerned, the most interesting genotype was the tetraploid one which showed resistance to both blackleg and tuber soft rot Significant correlations were found betweenE. carotovora subsp.atroseptica andE. carotovora subsp.carotovora both in resistance to blackleg and in resistance to tuber soft rot, suggesting that resistance to the two subspecies is controlled by the same gene/s or that the relevant genes are linked. On the other hand, correlations between blackleg and tuber soft rot were never significant, indicating that different mechanisms may control resistance in tuber and stems. Alternatively, the resistance could be controlled by the same gene/s under a different spatial/temporal expression pattern. Twelve diploid sexual hybrids and one tetraploid somatic hybrid were selected for resistance to blackleg and/or tuber soft rot and for tuber characteristics. 相似文献
56.
57.
Romina Beleggia Cristiano Platani Giuseppe Spano Massimo Monteleone Luigi Cattivelli 《Journal of Cereal Science》2009
Although pasta is generally not considered for its aromatic properties, some evidence proves that cereal flours release volatile compounds and they might have an effect on the aroma of the transformed products. This work reports on the characterization of the volatile components of semolina and pasta obtained from four durum wheat cultivars (Triticum durum Desf., cvs. PR22D89, Creso, Cappelli, Trinakria). Semolina samples were characterized through polar metabolite profiling and fatty acid analysis to identify potential precursors of the volatile components. The results show significant differences among the samples tested with cv. Trinakria characterized by the highest content of sugars and fatty acids. Volatile composition was investigated both in semolina and in cooked pasta using headspace solid-phase micro-extraction (HS-SPME) and identified by GC–MS. Thirty-five volatile compounds including aldehydes, ketones, alcohols, terpenes, esters, hydrocarbons and a furan were identified. Significant differences were observed between semolina and pasta samples in terms of composition and amount of the volatile compounds. During cooking an increase in aldehyde content, the appearance of ketones and a decrease in alcohol content were observed. Correlations between metabolites and volatiles demonstrate that the flavour of cooked pasta may differ significantly depending on the durum wheat cultivar employed. 相似文献
58.
Stefano Pavan Zheng Zheng Mariya Borisova Petra van den Berg Concetta Lotti Claudio De Giovanni Pim Lindhout Hans de Jong Luigi Ricciardi Richard G. F. Visser Yuling Bai 《Euphytica》2008,162(1):91-98
The recessive gene ol-2 confers papilla-associated and race-non-specific resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identified two simple sequence repeat (SSR) markers and one cleaved amplified polymorphic sequence (CAPS) marker which are linked to the resistance locus and co-dominantly inherited. Aiming to provide a base for ol-2 positional cloning, we used a large segregating F2 population to merge these markers with all the ol-2 linked amplified fragment length polymorphism (AFLP®) markers previously identified in an integrated genetic map. By screening a tomato bacterial artificial chromosome (BAC) library, we detected two BAC clones containing two expressed sequence tags (ESTs) homologous to the gene mlo, responsible for powdery mildew resistance in barley, as well as an ol-2-linked marker. Chromosomal mapping by Fluorescence in situ Hybridization (FISH) revealed major signals of the two BAC DNAs in the pericentromeric heterochromatin of the short arm of chromosome 4, in the same region where the ol-2 gene was previously mapped. The genetic and cytogenetic co-localisation between ol-2 and tomato mlo-homologue(s), in addition to the similarity of ol-2 and mlo resistances for both genetic and phytopathological characteristics, suggests that ol-2 is likely a mlo-homologue. Thus, a homology-based cloning approach could be more suitable than positional cloning for ol-2 isolation. 相似文献
59.
Transnasal administration of a combination of dexmedetomidine,midazolam and butorphanol produces deep sedation in New Zealand White rabbits
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60.