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排序方式: 共有127条查询结果,搜索用时 312 毫秒
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122.
Cryopreservation of Sheep Primordial Follicles 总被引:3,自引:0,他引:3
CA Amorim D Rondina CM Lucci A Giorgetti JR de Figueiredo PBD Gonçalves 《Reproduction in domestic animals》2007,42(1):53-57
The aim of this study was to evaluate the efficiency of 1 M dimethylsulphoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH) and glycerol (GLY) to cryopreserve primordial follicles. The first evaluation was performed soon after cryopreservation and the second evaluation after 4 days of in vitro culture, using the cryoprotectants that allowed the higher results (higher follicular survival rate) after cryopreservation. The results after follicular isolation (control) and cryopreservation using 1 M DMSO, EG, PROH and GLY showed that the mean number (+/- SEM) of live follicles per millilitre was 3204 (100%) +/- 319.27, 2798 (87%) +/- 239.14, 2492 (78%) +/- 345.8, 448 (14%) +/- 46.3 and 208 (7%) +/- 75.26, respectively. Higher follicular survival was reported when DMSO and EG were used. Control follicles and follicles cryopreserved with these two cryoprotectants were cultured and the percentage of follicular survival was 55% (control), 42% (EG) and 34% (DMSO). Similar results were found between control and follicles cryopreserved with EG. In conclusion, 1 M EG is the most effective cryoprotectant to preserve primordial follicles isolated from ovaries of sheep. 相似文献
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Technologies to edit the zygote genome have revolutionized biomedical research not only for the creation of animal models for the study of human disease but also for the generation of functional human cells and tissues through interspecies blastocyst complementation technology. The pig is the ideal species for these purposes due to its great similarity in anatomy and physiology to humans. Emerging biotechnologies require the use of oocytes and/or embryos of good quality, which might be obtained using in vitro production (IVP) techniques. However, the current porcine embryo IVP systems are still suboptimal and result in low monospermic fertilization and blastocyst formation rates and poor embryo quality. During recent years, intensive investigations have been performed to evaluate the influence of specific compounds on gametes and embryos and to avoid the use of undefined supplements (serum and serum derivate) in the incubation media. However, little consideration has been given to the use of the mineral oil (MO) to overlay incubation droplets, which, albeit being a routine component of the IVP systems, is a totally undefined and thus problematic product for the safety of gametes and embryos. In this review, we provide an overview on the advantages and disadvantages of using MO to cover the incubation media. We also review one important concern in IVP laboratories: the use of oils containing undetected contamination. Finally, we discuss the effects of different types of oils on the in vitro embryo production outcomes and the transfer of compounds from oil into the culture media. 相似文献
125.
CA Guymon EN Hoggan NA Clark TP Rieker DM Walba CN Bowman 《Science (New York, N.Y.)》1997,275(5296):57-59
Photopolymerizable diacrylate monomers dissolved in fluid-layer smectic A and smectic C liquid crystal (LC) hosts exhibited significant spatial segregation and orientation that depend strongly on monomer structure. Small, flexible monomers such as 1,6-hexanediol diacrylate (HDDA) oriented parallel to the smectic layers and intercalated, whereas rod-shaped mesogen-like monomers such as 1,4-di-(4-(6-acryloyloxyhexyloxy)benzoyloxy)-2-methylbenzene (C6M) oriented normal to the smectic layers and collected within them. Such spatial segregation caused by the smectic layering dramatically enhanced photopolymerization rates; for HDDA, termination rates were reduced, whereas for C6M, both the termination and propagation rates were increased. These polymerization precursor structures suggest novel materials-design paradigms for gel LCs and nanophase-separated polymer systems. 相似文献
126.
TC Johnson CA Scholz MR Talbot K Kelts RD Ricketts G Ngobi K Beuning I Ssemmanda JW McGill 《Science (New York, N.Y.)》1996,273(5278):1091-1093
Lake Victoria is the largest lake in Africa and harbors more than 300 endemic species of haplochromine cichlid fish. Seismic reflection profiles and piston cores show that the lake not only was at a low stand but dried up completely during the Late Pleistocene, before 12,400 carbon-14 years before the present. These results imply that the rate of speciation of cichlid fish in this tropical lake has been extremely rapid. 相似文献
127.
黑龙港流域微地貌与地下水埋深对土壤潜在盐渍化的影响 总被引:3,自引:0,他引:3
[目的]对近年河北省曲周县土壤潜在盐渍化进行多方位评价,为预防土壤盐渍化提供科学依据。[方法]通过微咸水灌溉试验、土壤剖面观测和土样分析,从海拔高度、土层深度、地下水埋深、土壤质地几个方面对黑龙港流域土壤盐分运移以及其对土壤潜在盐渍化的影响进行探讨。[结果]从区域角度分析,降雨和用微咸水灌溉加剧了表层盐分向下层淋洗的可能性,从而使土壤盐分多在40—100cm土层聚集。质地较为黏重的土层阻止了土壤盐分的运移而聚积在该土层之上,为土壤潜在盐渍化创造了条件。土壤盐分含量与海拔高度呈现出很好的相关性,其复相关指数R2=0.76。HCO_3~-的表聚现象比较明显,各土层土壤SO_4~(2-)离子和Ca~(2+)离子与全盐含量之间达到了显著和极显著相关(0.88*~1.00**,0.89*~0.97**),Ca~(2+)离子与SO_4~(2-)离子之间达到了显著和极显著相关(0.86*~0.97**)。[结论]微地貌和土体构型的变化将会影响到土壤盐分的重新分配,进而对土壤潜在盐渍化有重要影响。 相似文献