Effect of Golden Shiners on Plankton and Water Quality in Ponds Managed for Intensive Production

Water quality variables and plankton were monitored and compared between ponds that were stocked or not stocked with golden shiners in order to determine what changes occur in the plankton community and water quality of ponds managed for intensive production. Ponds with shiners had significantly lower phosphate and carbon dioxide concentrations and lower carbonate alkalinity than ponds without shiners. No significant difference in nitrite, nitrate, ammonia, temperature, secchi disk readings, or turbidity levels was found between the treatments. The standing crop of phytoplankton was nearly twice as great in ponds with fish. Ponds with fish had significantly fewer copepods but more rotifers than fishless ponds. Although the fish were offered a commercial feed with 29.5% protein and 1.5% crude fat at 3% of their biomass daily, they apparently continued to feed on crustacean zooplankton.     

Effects of trichlorfon, fenthion, and diflubenzuron on the zooplankton community and on production of reciprocal-cross hybrid striped bass fry in culture ponds

The application of trichlorfon, diflubenzuron, or fenthion to fertilized culture ponds stocked with 5-day-old, reciprocal-cross, hybrid striped bass fry resulted in an initial reduction in the concentration of rotifers and longer-term alteration of zooplankton successional stages, including changes in concentrations of rotifers, cladocerans, and copepods. Culture ponds without applied chemicals had the highest concentrations of small rotifers when fry were stocked, followed by high concentrations of cladocerans, copepod nauplii, and adult copepods. Fry survival in untreated ponds was higher than in chemically-treated ponds. Initial high concentrations of copepods in some ponds corresponded with low fry survival. Untreated ponds that were filled at the time of broodfish spawning, and stocked with fry 5 days later, had the highest fry survival rates, corresponding with peak rotifer concentrations, followed by a typical zooplankton succession.     

Pharmacokinetics of aditoprim in dogs after intravenous and oral administration: a preliminary study

The pharmacokinetics of aditoprim (2,4-diam-ino-5-[4-[dimethylamino]-3,5-dimethoxybenzyl] pyrimidine), a dihydrofolate reductase inhibitor, were studied in three eight to 17-month-old beagle dogs. The drug was alternately injected intravenously and administered orally as a tablet in two separate experiments. The doses ranged from 4–27 to 7–25 mg/kg bodyweight. The mean plasma clearance (CI) was 15-3 ml/min/kg and the mean volume of distribution (V_ss) 13-1 litres/kg. The mean plasma elimination half-life times after intravenous injection and oral administration were 11-6 and 9-6 hours, respectively. Aditoprim was readily (t_max= one to four hours) and completely absorbed from the intestinal tract (f = 89 per cent).     

Effect of a saponin-based surfactant and aging time on ruminal degradability of flaked corn grain dry matter and starch

The objectives of this study were to investigate the effect of a saponin-based surfactant, Grain Prep surfactant (GP), and hot flake aging time on starch characteristics and ruminal DM and starch degradability of steam-flaked corn grain. In 2 experiments, the moisture content of incoming corn was automatically adjusted using the Grain Prep Auto Delivery System to 19.8% (Exp. 1) and 18.5% (Exp. 2). The application rate of GP was 22 mg/kg (as-is basis). Control corn was treated with water alone. Processed corn in Exp. 2 was stored in insulated containers for 0, 4, 8, or 16 h. Flaked corn samples were incubated in the rumen of lactating dairy cows for 0, 2, 4, 6, 16, or 24 h. In Exp. 1, GP increased, compared with the control, the soluble fraction and effective degradability (ED) of DM by 17.2 and 8.6%, respectively. The ED of cornstarch was increased by 6.7%. In Exp. 2, the concentration of soluble DM and starch were increased by GP by 15 and 24% compared with the control. The ED of DM and starch were also increased by 3 and 4%, respectively. No differences in gelatinization temperatures were observed due to treatment, except that GP-treated grain had a slightly greater mean gelatinization enthalpy in Exp. 2. In a pilot study, DM degradability parameters were not affected by germination of the corn kernels. Aging of the hot flakes for up to 16 h resulted in a quadratic decrease in DM and starch ruminal degradability. The aging process affected starch gelatinization enthalpy values of flaked grain in a manner opposite to that observed for ruminal DM and starch degradation. This phenomenon was most likely explained by increased starch intramolecular associations or crystallinity associated with starch annealing, or both. This study confirmed our previous observations that Grain Prep surfactant increases flaked corn DM and starch degradability in the rumen. Because the rate of degradation was not affected by the surfactant, the increase in degradability was attributed mainly to increases in DM and starch solubility.     

Near‐infrared spectroscopy can predict the composition of organic matter in soil and litter

The usefulness and limitations of near‐infrared reflectance spectroscopy (NIRS) for the assessment of several soil characteristics are still not sufficiently explored. The objective of this study was to evaluate the ability of visible and near‐infrared reflectance (VIS‐NIR) spectroscopy to predict the composition of organic matter in soils and litter. Reflectance spectra of the VIS‐NIR region (400–2500 nm) were recorded for 56 soil and litter samples from agricultural and forest sites. Spectra were used to predict general and biological characteristics of the samples as well as the C composition which was measured by ¹³C‐CPMAS‐NMR spectroscopy. A modified partial least‐square method and cross‐validation were used to develop equations for the different constituents over the whole spectrum (1st to 3rd derivation). Near‐infrared spectroscopy predicted well the C : N ratios, the percentages of O‐alkyl C and alkyl C, the ratio of alkyl C to O‐alkyl C, and the sum of phenolic oxidation products: the ratios of standard deviation of the laboratory results to standard error of cross‐validation (RSC) were greater than 2, the regression coefficients (a) of a linear regression (measured against predicted values) ranged from 0.9 to 1.1, and the correlation coefficients (r) were greater than 0.9. Satisfactorily (0.8 ≤ a ≤ 1.2, r ≥ 0.8, and 1.4 ≤ RSC ≤ 2.0) assessed were the contents of C, N, and production of DOC, the percentages of carbonyl C and aromatic C and the ratio of alkyl C to aromatic C. However, the N‐mineralization rate and the microbial biomass were predicted unsatisfactorily (RSC < 1.4). The good and satisfactory predictions reported above indicate a marked usefulness of NIRS in the assessment of biological and chemical characteristics of soils and litter.     

Differences in the chemical composition of dissolved organic matter from waste material of different sources

The chemical composition of waste-material-derived dissolved organic matter (DOM) was characterized by chemolytic analyses and ¹H, ¹³C and ³¹P nuclear magnetic resonance (NMR) spectroscopy. Dissolved organic matter was extracted by water from an aerobic fermented urban waste compost, a sewage sludge and a pig slurry and then fractionated using the XAD-8 method. The amount of water-extractable dissolved organic carbon (DOC) ranged from 3% in the sewage sludge to 22% in the pig slurry. Dissolved organic matter isolated from pig slurry was equally distributed between hydrophilic and hydrophobic DOC, whereas in the sewage-sludge-derived material the hydrophobic fraction was predominant. Dissolved organic C from the urban waste compost was mainly within the hydrophilic fraction. Wet-chemical analysis and ¹H- and ¹³C-NMR spectra showed that both DOM fractions from the urban waste compost were low in neutral, acidic and amino sugars as well as in lignin-derived compounds. In turn, the materials were rich in low-molecular-weight aliphatic compounds. The chemical structure of both fractions is probably the result of the intensive transformation of urban waste compost during its fermentation. The hydrophilic fractions of DOM from sewage sludge and pig slurry contained considerable amounts of carbohydrates but were also rich in low-molecular-weight aliphatics. The respective hydrophobic fractions had the largest contents of CuO-extractable phenols which may in part derive from sources other than lignin. By contrast with the other materials, the hydrophobic fraction from the pig slurry seemed to contain polymeric rather than low-molecular-weight material. The ³¹P-NMR spectrum of the hydrophilic DOM fraction from urban waste compost did not show signals of inorganic or organic P compounds while the spectrum of the hydrophobic fraction revealed traces of monoester P, diester P, and orthophosphate. ³¹P-NMR spectroscopy suggested that both the hydrophobic and hydrophilic fractions from pig slurry did not contain organic P. The hydrophilic DOM fraction from sewage sludge contained orthophosphate, organic monoester P and a little pyrophosphate. The hydrophobic fraction contained mainly organic diester P and smaller amounts of teichoic acids and organic monoester P. Considering that water-soluble fractions of urban waste compost contained no easily plant-available P and a low content of labile organics, we conclude that this material contains less labile nutrients and is more refractory than the soluble constituents of pig slurry and sewage sludge.     

Variability of CO2 and N2O emissions during freeze‐thaw cycles: results of model experiments on undisturbed forest‐soil cores

The amounts of N₂O released in periods of alternate freezing and thawing depend on site and freezing conditions, and contribute considerably to the annual N₂O emissions. However, quantitative information on the N₂O emission level of forest soils in freeze‐thaw cycles is scarce, especially with regard to the direct and indirect effect of tree species and the duration of freezing. Our objectives were (i) to quantify the CO₂ and N₂O emissions of three soils under beech which differed in their texture, C and N contents, and humus types in freeze‐thaw cycles, and (ii) to study the effects of the tree species (beech (Fagus sylvatica L.) and spruce (Picea abies (L.) Karst.)) for silty soils from two adjacent sites and the duration of freezing (three and eleven days) on the emissions. Soils were adjusted to a matric potential of –0.5 kPa, and emissions were measured in 3‐hr intervals for 33 days. CO₂ emissions of all soils were similar in the two freeze‐thaw cycles, and followed the temperature course. In contrast, the N₂O emissions during thawing differed considerably. Large N₂O emissions were found on the loamy soil under beech (Loam‐beech) with a maximum N₂O emission of 1200 μg N m^–2 h^–1 and a cumulative emission of 0.15 g N m^–2 in the two thawing periods. However, the sandy soil under beech (Sand‐beech) emitted only 1 mg N₂O‐N m^–2 in the two thawing periods probably because of a low water‐filled pore space of 44 %. The N₂O emissions of the silty soil under beech (Silt‐beech) were small (9 mg N m^–2 in the two thawing periods) with a maximum emission of 150 μg N m^–2 h^–1 while insignificant N₂O emissions were found on the silty soil under spruce (0.2 mg N m^–2 in the two thawing periods). The cumulative N₂O emissions of the short freeze‐thaw cycles were 17 % (Sand‐beech) or 22 % (Loam‐beech, Silt‐beech) less than those of the long freeze‐thaw cycles, but the differences between the emissions of the two periods were not significant (P ≤ 0.05). The results of the study show that the amounts of N₂O emitted in freeze‐thaw cycles vary markedly among different forest soils and that the tree species influence the N₂O thawing emissions in forests considerably due to direct and indirect impacts on soil physical and chemical properties, soil structure, and properties of the humus layer.