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101.
Saleska SR Miller SD Matross DM Goulden ML Wofsy SC da Rocha HR de Camargo PB Crill P Daube BC de Freitas HC Hutyra L Keller M Kirchhoff V Menton M Munger JW Pyle EH Rice AH Silva H 《Science (New York, N.Y.)》2003,302(5650):1554-1557
The net ecosystem exchange of carbon dioxide was measured by eddy covariance methods for 3 years in two old-growth forest sites near Santarém, Brazil. Carbon was lost in the wet season and gained in the dry season, which was opposite to the seasonal cycles of both tree growth and model predictions. The 3-year average carbon loss was 1.3 (confidence interval: 0.0 to 2.0) megagrams of carbon per hectare per year. Biometric observations confirmed the net loss but imply that it is a transient effect of recent disturbance superimposed on long-term balance. Given that episodic disturbances are characteristic of old-growth forests, it is likely that carbon sequestration is lower than has been inferred from recent eddy covariance studies at undisturbed sites. 相似文献
102.
Rock FL Mao W Yaremchuk A Tukalo M Crépin T Zhou H Zhang YK Hernandez V Akama T Baker SJ Plattner JJ Shapiro L Martinis SA Benkovic SJ Cusack S Alley MR 《Science (New York, N.Y.)》2007,316(5832):1759-1761
Aminoacyl-transfer RNA (tRNA) synthetases, which catalyze the attachment of the correct amino acid to its corresponding tRNA during translation of the genetic code, are proven antimicrobial drug targets. We show that the broad-spectrum antifungal 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690), in development for the treatment of onychomycosis, inhibits yeast cytoplasmic leucyl-tRNA synthetase by formation of a stable tRNA(Leu)-AN2690 adduct in the editing site of the enzyme. Adduct formation is mediated through the boron atom of AN2690 and the 2'- and 3'-oxygen atoms of tRNA's3'-terminal adenosine. The trapping of enzyme-bound tRNA(Leu) in the editing site prevents catalytic turnover, thus inhibiting synthesis of leucyl-tRNA(Leu) and consequentially blocking protein synthesis. This result establishes the editing site as a bona fide target for aminoacyl-tRNA synthetase inhibitors. 相似文献
103.
104.
Elephants are highly social beings with complex individual personalities. We know that elephants have a general interest in death, investigating carcasses, not just limited to kin; however, research does not explore in depth whether individuals change their behavior or personality following traumatic events, such as the death of a conspecific. Within a captive herd of Asian elephants (Elephas maximus) housed at Chester Zoo, UK, we measured social behavior and proximity and personality using the Ten-Item Personality Inventory, and found age-related and relationship-related changes in both behavior and personality following the deaths of herd members. Overall, the herd spent less time socializing and engaging in affiliative behaviors following the death of the adult female when compared to baseline data, yet spent more time engaging in these behaviors after the death of two calves. The death of the central female had a dramatic impact on her infant calf, resulting in increasingly withdrawn behavior, yet had the opposite effect on her adult daughter, who subsequently established a more integrated role within the herd. Emotional Stability fell in the motherless calf but rose in an adult female, who had lost her adult daughter, but had a new calf to care for. We suggest that the greater impact on the behavior and personality of surviving herd members following the deaths of calves, compared to an adult member, attests to the significance of the unifying role played by calves within an elephant herd. 相似文献
105.
Lucy J. Sheppard Alan Crossley Judith Parrington Francis J. Harvey J. Neil Cape 《Water, air, and soil pollution》2001,130(1-4):953-958
Effects of enhanced N, S and NS Acid additions, up to approximately 100 kg N and S ha?1 y?1, are described for a 15-year-old Sitka spruce forest growing on an acid peat in Scotland. Groups of 10 trees, replicated over 4 blocks, have been treated at canopy height on approximately 50 or 100 occasions with 2 mm precipitation equivalent, between April and November, since 1996. Relative stem volume increment (RSVI) has been consistently higher in the NS Acid treated trees compared with control or N treated trees since the second year of treatment, although no dose response was found. Litterfall was also considerably increased in the NS Acid treatments and showed a clear dose effect but was not correlated with RSVI. Base cation concentrations in soil waters, collected using zero tension lysimeters reflected the presence or absence of the spray treatments and showed a dose related increase in response to NS Acid inputs. Treatment with 2NS Acid increased NH4-N and NO3-N by an order of magnitude. Results after 4 years of treatment showed a positive growth and litterfall response to NS Acid inputs but no effect of N alone. Enhanced stemwood growth may be linked to the higher base cation and phosphate concentrations measured in the soil water in the presence of NS Acid and S treatments. 相似文献
106.
A new furostanol pentaoligoside and spirostanol tetraoligoside were isolated for the first time from yam tubers (Dioscorea pseudojaponica Yamamoto) from Taiwan, together with four known yam saponins, methyl protodioscin, methyl protogracillin, dioscin, and gracillin. Their structures were characterized as 26-O-beta-D-glucopyranosyl-22alpha-methoxyl-(25R)-furost-5-en-3beta,26-diol, 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-([alpha-L-rhamnopyranosyl-(1-->4)]-O-[alpha-L-rhamnopyranosyl-(1-->4)])-beta-D-glucopyranoside, and (25R)-spirost-5-en-3beta-ol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-([alpha-L-rhamnopyranosyl-(1-->4)]-O-[alpha-L-rhamnopyranosyl-(1-->4)])-beta-D-glucopyranoside. The structural identification was performed using LC-MS and 1H and 13C NMR. The methanol extract of yam tubers was fractionated by XAD-2 column chromatography using a methanol/water gradient elution system to yield furostanol and spirostanol glycoside fractions. Preparative high-performance liquid chromatography, employing a C18 column and a mobile phase of methanol/water (69:31, v/v), was used to separate each furostanol glycoside, whereas a mobile phase of methanol/water (79:21, v/v) was used to resolve the individual spirostanol glycosides. The conversions from steroid saponins to diosgenin after acid hydrolysis were around 68 and 90% for furostanol and spirostanol glycosides, respectively. 相似文献
107.
Steph Bennington Will Rayment Rohan Currey Lucy Oldridge Shaun Henderson Marta Guerra Tom Brough David Johnston Chloe Corne David Johnson Liz Slooten Steve Dawson 《水产资源保护:海洋与淡水生态系统》2021,31(3):665-676
- A small population of approximately 68 bottlenose dolphins, Tursiops truncatus, resident in Doubtful Sound, New Zealand, is subject to physiologically challenging conditions, and is exposed to anthropogenic pressure from tourism.
- A voluntary Code of Management incorporating dolphin protection zones (DPZs), in which tour boat access is limited, was established in 2008.
- Kernel density estimation (KDE) was used to quantify dolphin habitat use over a 13-year period in order to describe seasonal variation in habitat use and consistency of habitat use over a decadal period, and to provide quantitative estimates of the extent of overlap between DPZs and core areas (50% volume contour) of habitat use.
- Habitat use varied seasonally, with the inner fjord area used more frequently in warmer months, and with a shift in use to the outer fjord in colder months. Patterns in habitat use were highly consistent over the 13-year duration of the study.
- The spatial overlap between the area of core dolphin habitat and DPZs was low (<18%) overall, and some DPZs were rarely used during colder periods.
- Consistency in habitat use through time vindicates spatial management, but low overlap between core habitat and current DPZs suggests that an expansion of the DPZ areas would confer greater protection.
108.
109.
Sesame seeds (Sesamum indicum L.) are unique because of potent and various physiological activities imparted by their bioactive lignans. This investigation studied the intestinal distribution and excretion of sesaminol in Sprague-Dawley (SD) rats. To investigate the distribution of sesaminol (per oral 220 mg/kg), the changes in concentration of sesaminol and its metabolites were determined in the intestines and plasma within the 24 h period after tube feeding of sesaminol to SD rats. Results show that the epimerization of sesaminol appeared to be catalyzed by acid in the simulated gastric fluids. The major sesaminol epimer was characterized as 2-episesaminol using 2D-NMR. These findings indicate that sesame sesaminol and its epimer are poorly absorbed prior to reaching the rectum and that substantial amounts pass from the small to the large intestine, where they are metabolized by the colonic microflora to tetrahydrofuranoid metabolites. Sesaminol in plasma was largely present as phase II conjugates, and the seven metabolites were detected as the 2-episesaminol, sesaminol-6-catechol, methylated sesaminol-catechol, R,R-hydroxymethylsesaminol-tetrahydrofuran, S,R-hydroxymethylsesaminol-tetrahydrofuran, enterolactone, and enterodiol. Excretions of sesaminol in urine and feces within the 24 h period were equivalent to 0.02 and 9.33% of the amount ingested, respectively. 相似文献
110.
Pauline M. Currey David Johnson René van der Wal Lucy J. Sheppard Rebekka R.E. Artz 《Soil biology & biochemistry》2011,43(3):495-502
To understand the implications of atmospheric nitrogen deposition on carbon turnover in peatlands, we conducted a 13C pulse labeling experiment on Calluna vulgaris and Eriophorum vaginatum already receiving long-term (5 years) amendments of 56 kg N ha−1 y−1 as ammonium or nitrate. We examined shoot tissue retention, net ecosystem respiration returns of the 13C pulse, and soil porewater DOC content under the two species. 13C fixation in Eriophorum leaves was enhanced with nitrogen addition and doubled with nitrate supply. This newly fixed C appeared to be relocated below-ground faster with nitrogen fertilization as respiration returns were unaffected by N inputs. By contrast, increases in 13C fixation were not observed in Calluna. Instead, net ecosystem respiration rates over Calluna increased with N fertilization. There was no significant label incorporation into DOC, suggesting a conservative strategy of peatland vegetation regarding allocation of C through root exudation. Greater concentrations of total DOC were identified with nitrate addition in Calluna. Given the long-term nature of the experiment and the high N inputs, the overall impacts of nitrogen amendments on the fate of recently synthesized C in Eriophorum and Calluna in this ombrotrophic peatland were surprisingly more moderate than originally hypothesized. This may be due to N being effectively retained within the bryophyte layer, thus limiting, and delaying the onset of, below-ground effects. 相似文献