运输应激猪骨骼肌中热应激蛋白HSP70和HSP90家族的表达

利用Western blot技术，检测长途运输试验猪骨骼肌（背最长肌和臀长肌）中分属于HSP70家族和HSP90家族的4种应激蛋白（HSP70，HSP72，HSP86和HSP90）的表达，所有运输应激猪和对照猪肌肉组织 中均检测到了上述4种HSP，对照猪组织中HSP的表达说明HSP除了在受应激 的细胞内行使生理功能外，还具有独立于应激刺激应答以外的其它作用，6h长途运输后，HSP的表达量明显不同，HSP70和HSP72在肌肉组织中的表达虽然有一定的增加，但统计学分析差异不显（P＞0．05），而HSP86和HSP90在骨髓肌中的表达明显下降，尤以HSP90下降最显（P＜0．01），提示HSP90家族成员与肉品品质相关，可能作为判应激损伤的的指征。     

Energy and Resources: A plan is outlined according to which solar and wind energy would supply Denmark's needs by the year 2050

Two possible futures for the industrial world may be distinguished: (i) Large amounts of low-cost energy become available and the more energy-intensive methods for extracting resources from lowergrade deposits continue to sustain industrial expansion until either the environmental impact becomes unacceptable or ultimate limits, such as climate disruptions, put an end to such growth. (ii) The cost of nonrenewable energy resources continue to rise, but a fixed amount of energy from continuous sources may be utilized at constant cost. In this case a lower production level may be set by the amount of energy that is available from renewable sources, and society may thus have to be reshaped with energy economization in focus. If it is possible to choose between these two alternatives, the choice should be based on a discussion of the pros and cons of each one, and in particular on the desirability of having to process an increasing fraction of the earth's crust in search of raw materials in order to maintain growth as long as possible. However, the availability, of the first option is far from certain and it thus seems reasonable to plan for the second alternative. I have tried to propose such a plan for a small, homogeneous geographical region, namely Denmark. The ceiling on the consumption of energy from continuous sources is chosen in accordance with the criterion of not having to convert a major part of the land area to energy-collecting systems. The proposed annual average energy consumption of 19 gigawatts by the year 2050 corresponds to solar energy collecting panels (in use only 50 percent of the time) with an area of roughly 180 square kilometers and a windmill swept area of about 150 square kilometers. These (vertical) areas constitute less than 1 percent of the total land area. The selection of solar or wind energy for different applications has been based on known technology and may be subject to adjustments. The project has been shown to be economically feasible according to estimates of the cost of various alternatives during the 25-year depreciation period adopted. However, the initial cost per energy unit produced is higher than that for most of the alternatives, so that action is not expected to be taken immediately as a result of purely private initiative. In a public economic evaluation, other factors must be considered in addition to the cost of energy per kilowatt-hour. At present, Denmark has over 10 percent of its labor force out of employment and a substantial deficit on its balance of payments, so that an early start on the solar and wind energy project, based on national industry, would have additional payoffs compared with energy systems based on imported technology or imported fuels. Several factories that are now being closed down as a result of the economic crisis could be adapted to the production of parts for solar or wind power systems, and the building industry, badly hit by unemployment, would receive legitimate work.     

Ellipsosomes: organelles containing a cytochrome-like pigment in the retinal cones of certain fishes

Ellipsosomes are dense spherical bodies containing a very large concentration of a heme pigment spectroscopically resembling pure cytochrome c. They are located at the outer ends of the inner segments of the cones of certain fishes. Although, superficially, they resemble the similarly located oil droplets in the cones of birds and reptiles, their ultrastructure and staining properties resemble those of the neighboring mitochondria. However, like the oil droplets, they may serve as intracellular color filters.     

Protein deficiency and tribal warfare in Amazonia: new data

Increasing numbers of anthropological studies about native Amazonian warfare and demographic practices attempt to explain these phenomena as competition over or a response to scarce game animals and other sources of high-quality protein. Recently completed field research among the Yanomam? Indians living at the Venezuela-Brazil border indicates that their protein intake is comparable to that found in highly developed industrialized nations and as much as 200 percent more than many nutritional authorities recommend as daily allowances. Recent data on other Amazonian tribes likewise fails to indicate a correlation between protein intake and intensity of warfare patterns.     

Evolution of sea surface temperature in the somali basin during the southwest monsoon of 1979

Satellite and research vessel observations of sea surface temperature during the southwest monsoon of 1979 show the development of large wedge-shaped areas of cold water along the Somali coast at both 5 degrees and 10 degrees N during June and July. The cold water associated with the large northern and southern Somali eddy systems could be traced several hundred kilometers offshore. By late August the cold wedge at 5 degrees N translated northeastward as far as 10 degrees N at speeds of 15 to 30 centimeters per second, indicating a coalescence of the systems.     

Oxygen-18 enrichment of planktonic foraminifera due to gametogenic calcification below the euphotic zone

Empty shells of spinose planktonic foraminifera on the seabed are significantly enriched in oxygen-18 as compared with the shells of their living counterparts in surface waters. This enrichment is due to gametogenic calcification, which extracts calcium carbonate from deeper and colder waters as the shell sinks below the euphotic zone. JEAN-CLAUDE DUPLESSY.     

Complementary DNA sequencing: expressed sequence tags and human genome project

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.     

The silica balance in the world ocean: a reestimate

The net inputs of silicic acid (dissolved silica) to the world ocean have been revised to 6.1 +/- 2.0 teramoles of silicon per year (1 teramole = 10(12) moles). The major contribution (about 80 percent) comes from rivers, whose world average silicic acid concentration is 150 micromolar. These inputs are reasonably balanced by the net ouputs of biogenic silica of 7.1 +/- 1.8 teramoles of silicon per year in modern marine sediments. The gross production of biogenic silica (the transformation of dissolved silicate to particulate skeletal material) in surface waters was estimated to be 240 +/- 40 teramoles of silicon per year, and the preservation ratio (opal accumulation in sediment/gross production in surface waters) averages 3 percent. In the world ocean the residence time of silicon, relative to total biological uptake in surface waters, is about 400 years.     

Rapid wastage of Alaska glaciers and their contribution to rising sea level

We have used airborne laser altimetry to estimate volume changes of 67 glaciers in Alaska from the mid-1950s to the mid-1990s. The average rate of thickness change of these glaciers was -0.52 m/year. Extrapolation to all glaciers in Alaska yields an estimated total annual volume change of -52 +/- 15 km3/year (water equivalent), equivalent to a rise in sea level (SLE) of 0.14 +/- 0.04 mm/year. Repeat measurements of 28 glaciers from the mid-1990s to 2000-2001 suggest an increased average rate of thinning, -1.8 m/year. This leads to an extrapolated annual volume loss from Alaska glaciers equal to -96 +/- 35 km3/year, or 0.27 +/- 0.10 mm/year SLE, during the past decade. These recent losses are nearly double the estimated annual loss from the entire Greenland Ice Sheet during the same time period and are much higher than previously published loss estimates for Alaska glaciers. They form the largest glaciological contribution to rising sea level yet measured.