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51.
Araújo FD Esper LM Kuaye AY Sircili MP Marsaioli AJ 《Journal of agricultural and food chemistry》2012,60(2):585-592
A chemical study of acyl-homoserine lactones (acyl-HSLs) produced by Enterobacter sakazakii resulted in the identification of three molecules: (S)-N-heptanoyl-HSL, (S)-N-dodecanoyl-HSL and (S)-N-tetradecanoyl-HSL. Mixed cultures of E. sakazakii and Bacillus cereus depleted E. sakazakii acyl-HSLs, suggesting acyl-HSL degradation by B. cereus hydrolases (hydrolysis of the lactone or amide moiety). The expression of B. cereus acyl-HSL lactonase and acyl-homoserine acylase was confirmed by monitoring the biotransformation of (S)-N-dodecanoyl-HSL into (S)-N-dodecanoyl-homoserine, dodecanoic acid and homoserine in the presence of B. cereus whole cells, using electrospray-mass spectrometry (ESI-MS). 相似文献
52.
Bandini LA Neto AF Pena HF Cavalcante GT Schares G Nishi SM Gennari SM 《Veterinary parasitology》2011,176(2-3):151-156
Neospora caninum is widely distributed in the world and this parasite is one of the major causes of abortion in cattle. Dogs and coyotes are definitive hosts of N. caninum and several species of domestic and wild animals are intermediate hosts. Dogs can become infected by the ingestion of tissues containing cysts and then excrete oocysts. It is not yet known whether sporulated oocysts are able to induce a patent infection in dogs, i.e. a shedding of N. caninum oocysts in feces. The objective of this study was to experimentally examine the infection of dogs by sporulated oocysts. The oocysts used in the experiment were obtained by feeding dogs with brain of buffaloes (Bubalus bubalis) positive for anti-N. caninum antibodies by indirect fluorescent antibody test (IFAT ≥200). Oocysts shed by these dogs were confirmed to be N. caninum by molecular methods and by bioassay in gerbils, and sporulated N. caninum oocysts were used for the oral infection of four dogs. The dogs were 8 weeks old and negative for antibodies to N. caninum and Toxoplasma gondii. Dogs 1 and 4 received an inoculum of 10,000 sporulated oocysts each; dog 2 an inoculum of 5000 sporulated oocysts and dog 3 received 1000 sporulated oocysts of N. caninum. The total feces excreted by these dogs were collected and examined daily for a period of 30 days. No oocysts were found in their feces. The dogs were monitored monthly for a 6-month period to observe a possible seroconversion and when this occurred the animals were eliminated from the experiment. Dogs 1 and 4 seroconverted 1 month after the infection with titer, in the IFAT, of 1600 and 800, respectively; the other two dogs presented no seroconvertion during the 6-month period. Dogs 1 and 2 were euthanized 180 days after infection and were examined for the detection of N. caninum in tissues (brain, muscle, lymph node, liver, lung, heart and bone marrow) by immunohistochemistry and PCR with negative results in both techniques. Bioassay in gerbils with brain of these dogs was also performed and again the results were negative. In conclusion, dogs infected with sporulated oocysts of N. caninum were not able to shed oocysts in feces. However, a higher dose of infection stimulated the production of antibodies against N. caninum in the dogs. 相似文献
53.
Nagamine MK Sanches DS Pinello KC Torres LN Mennecier G Latorre AO Fukumasu H Dagli ML 《Veterinary research communications》2011,35(6):391-399
Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors
present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an
antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer
cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and
treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate
cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable
cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction
of G0/G1 and G2/M –phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent
for the treatment of canine MCT. 相似文献
54.
Soares Camila Rocha Júnior Vicente Ribeiro Monção Flávio Pinto Borges Lucas Daniel Alcântara Caldeira Luciana Albuquerque Costa Natanael Mendes Ruas José Reinaldo Mendes Rigueira João Paulo Sampaio da Cunha Siqueira Carvalho Cinara de Sales Eleuza Clarete Junqueira de Assis Pires Daniel Ananias Chamone Julieta Maria Alencar 《Tropical animal health and production》2020,52(5):2567-2576
Tropical Animal Health and Production - The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals’ diets can... 相似文献
55.
56.
Karina Batista Alessandra Aparecida Giacomini Luciana Gerdes Waldssimiler Teixeira De Mattos Ivani Pozar Otsuk 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(7):567-577
ABSTRACT The corn-grass intercropping system began to be used in the dry season, as it provides increased straw production for the no-tillage system. However, corn and grass are plants demanding in nitrogen. The corn cultivated in the dry season is called out-of-season corn in Brazil. The objective of this study was to evaluate the productivity and nutrients concentrations of out-of-season corn and Congo-grass intercropped in the no-tillage system, as a function of the nitrogen rates applied in sidedressing. The treatments were four nitrogen rates, 0, 30, 60 and 90?kg?ha?1. The experimental design was randomised complete blocks with four replicates. The experimental period corresponded to the harvests of 2015 and 2016. There were evaluated: corn grain yield, dry mass production and nutrient concentration in the shoots of the plants intercropped. The evaluations were carried out at the physiological maturity stage of the corn and at the time of desiccation of the grass. Nitrogen increased grain yield and dry mass production of the plants intercropped. Nitrogen maximised the magnesium concentration in out-of-season corn and the nitrogen, calcium, magnesium and sulphur concentrations in Congo grass. Excess nitrogen promoted reduction of the phosphorus in the Corn-Congo grass intercropping system in the dry season. 相似文献
57.
do Amaral Ana Cludia Tenrio de Holanda Cavalcanti Maciel Marlia Machado Alexandre Reis de Oliveira Luciana Gonalves Lima Cristiano Souza da Costa Antonio Flix de Oliveira Neiva Tinti 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):875-890
European Journal of Plant Pathology - This study aimed to evaluate the potential antagonistic role of Trichoderma in the control of Fusarium isolates belonging to the Fusarium oxysporum species... 相似文献
58.
Molecular and morphological characterization of Golestan (Iran) olive ecotypes provides evidence for the presence of promising genotypes 总被引:1,自引:1,他引:0
Soraya Mousavi Mehdi Hosseini Mazinani Kazem Arzani Abbas Ydollahi Saverio Pandolfi Luciana Baldoni Roberto Mariotti 《Genetic Resources and Crop Evolution》2014,61(4):775-785
Unidentified olive plants naturally grow in the Golestan province of Iran, on different soils and under climates spanning from sub-temperate to desert conditions, represented by single trees or groups of few trees. We collected samples from representative sites and analyzed them by simple sequence repeat markers in order to determine their identity and their relationships to prominent Iranian and Mediterranean reference cultivars. Population structure analysis separated these ecotypes from Mediterranean and, surprisingly, from all Iranian cultivars, the parentage test excluded their direct contribution as candidate parents or offspring of cultivars, and they also showed a high level of admixture. Their differentiation from cultivated olives may be attributed to different factors: they could represent wild plants or could derive from natural dissemination of ancestral cultivated trees. Their survival up to now may be due to the fact that most of them are grown on sacred sites such as necropolis. Anyhow, the adaptation to strong environmental stresses, and their fruit size and oil content make the olive Golestan ecotypes a valuable source of genetic variation previously uncharacterized and currently threatened with extinction. 相似文献
59.
The importance of the role of local community forestry institutions towards forest conservation is exemplified through a comparison of two adjacent areas within the Central Yucatan Peninsular Region (CYPR) in which Land-Use Cover Change (LUCC) analyses were conducted. We also used logistic regression analyses to examine key environmental, socioeconomic and institutional drivers associated with deforestation. One of the areas, La Montaña (LM) at Hopelchen, Campeche, is part of the northern section and buffer zone of the Calakmul Biosphere Reserve (CBR) as well as part of the Mesoamerican Biological Corridor. LM is an agricultural forest frontier region economically dependent on productive activities. The other study, Zona Maya (ZM), found at the municipality of Felipe Carrillo Puerto in Quintana is characterized by having developed community-based forestry enterprises with world wide recognition. In addition, the major tourism industry in nearby Cancun and Riviera Maya provide an important source of off-farm labor and temporary migration. Results show contrasting annual deforestation rates among the two study areas, being greater in the Campeche site (0.7% from 2000 to 2005) compared to Quintana Roo (−0.002% from 2000 to 2004). Logistic regression results show that the occurrence of land clearing at LM is significantly related to demographic variables as well as soil-environment types and conservation status did not reveal to relate to LUCC processes at all. At Quintana Roo, despite twice the population density, population variables at large did not strongly correlate to forest clearing. Rather forest conservation or maintenance was shown to be influenced by local community forestry institutions and a landscape zoning provided by larger management goals from the part of the communities. Greater availability of wage labor for the prominent tourism economy may also reflect a lesser dependence on agriculture and contribute to the much lower deforestation rate. Nonetheless, results show that community forest management can play an effective role in forest conservation. We argue for a regional land use management approach as a conservation strategy in which local inhabitants are considered key actors. 相似文献
60.
de Almeida Daniel Mageste Marcondes Marcos Inácio Rennó Luciana Navajas Pereira Silva Luiz Henrique Martins Leandro Soares Marquez David Esteban Contreras Castaño Villadiego Faider Alberto Velez Saldarriaga Felipe Franco Julian David Castaño Moreno Deilen Paff Sotelo de Moura Felipe Henrique Paulino Mário Fonseca 《Tropical animal health and production》2019,51(1):79-87
Tropical Animal Health and Production - The objective of this study was to evaluate the effects of strategic supplementation in the dry period and dry/rainy transition period on the performance and... 相似文献