Enhanced Bacillus thuringiensis Production from Sewage Sludge with Alkaline and Ultrasonic Pretreatments

Sewage sludge is a cost-effective media for the production of Bacillus thuringiensis (Bt) based biopesticides. To enhance the entomotoxicity of the fermentation broth, pretreatments of sewage sludge by alkali and ultrasonic were applied in this study. Effects of alkaline and ultrasonic pretreatments on the soluble COD (SCOD) and total COD (TCOD) were evaluated by altering the alkali addition dose and the ultrasonic specific energy. Suitable pretreatment conditions were optimized with 5 g l^?1 sodium hydroxide (NaOH) for alkaline treatment and 1.2?×?10⁵ kJ kg^?1 of total solid for ultrasonic treatment. Fermentations of raw and pretreated sludge for biopesticides were carried out in a bench scale fermentor. Results revealed that both pretreatments were effective for Bt growth and metabolism. Higher viable cells (VC) and viable spores (VS) counts, δ-endotoxin yields and entomotoxicity were achieved in the pretreated sludge. The enhancement was attributed to more available nutrients and better oxygen transfer. Moreover, ultrasonic pretreated sludge was superior to alkaline pretreated sludge for δ-endotoxin production and entomotoxicity owing to its higher soluble C/N ratio and finer particles.     

农业土壤表面不平度和测量与分析

农业土壤表面不平度与土壤风蚀、行走装置的相互作用和田间土壤含水量测定分布图等方面具有极其重要的作用.本文利用激光式不平度测试仪测定犁耕耕地地面、圆盘耙地地面和驱动耙耙地地面的不平度,测定地面不平度沿着三个方向进行,即平行于耕作方向、倾斜于耕作方向和垂直于耕作方向.对每一种地面不平度的RMS值、相关长度和自相关函数进行了分析,结果表明对于短的地块,可用单一尺度来表征地表不平度的特征,地表不平度特征属于指数相关函数,RMS高度和自相关长度具有明显的相关性.对于长的地块,平整的农业地表不平度具有分形性或多尺度性,其自相关函数是一种分形过程.     

Carbon flux from decomposing 13C-labeled transgenic and nontransgenic parental rice straw in paddy soil

Purpose

Genetic modification of Bt rice may affect straw decomposition and soil carbon pool under flood conditions. This study aims to assess the effects of cry gene transformation in rice on the residue decomposition and fate of C from residues under flooded conditions.

Materials and methods

A decomposition experiment was set up using ¹³C-enriched rice straws from transgenic and nontransgenic Bt rice to evaluate the soil C dynamics and CH₄ or CO₂ emission rates in the root and non-root zones. The concentrations and stable carbon isotope compositions of the soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass carbon (MBC), CH₄, and CO₂ of the root and non-root zones were determined from 7 to 110 days after rice straw incorporation.

Results and discussion

Rice straw incorporation into soil significantly increased the SOC, DOC, and MBC concentrations and the CH₄ and CO₂ emission rates. The percentage of ¹³C-SOC remaining in the root zone was significantly lower than that in the non-root zone with rice straw decomposition. The DOC and MBC concentrations significantly increased in both the root and non-root zones between 0 and 80 days after rice straw incorporation. However, no significant differences were found after Bts (Bt rice straw added into soil) and Cks (nontransgenic Bt rice straw added into soil) incorporation in the root and non-root zones. This result may be attributed to the priming effects of sufficient oxygen and nutrients on straw degradation in the root zone.

Conclusions

Bt gene insertion did not affect the SOC, DOC, and MBC concentrations and the CH₄ and CO₂ emission rates in both the root and non-root zones. However, rice straw incorporation and root exudation significantly increased the SOC, DOC, and MBC concentrations and the CH₄ and CO₂ emission rates.     

塿土旱作区施肥对小麦产量和水分利用效率的影响

研究了黄土高原南部塿土旱作区施肥对小麦产量和水分利用效率的影响。结量表明,合理增施肥料能显著提高冬小麦产量和水分利用效率,最高施肥量分别提高了1611kg/hm~2和0.235kg/mm水分。施肥量增加使小麦叶片水势有所降低,0～200cm土层贮水量呈发育前期升高而后期下降的趋势,改善了小麦植株自身调节和保持水分的能力,提高了产量和水分利用效率。     

鸽新城疫油乳剂灭活苗的研制与应用

利用从当地分离的鸽新城疫病毒，研制了鸽新城疫灭活油乳剂苗。此种疫苗使用后，接种鸽无任何不良反应；免疫14d后攻毒，保护率可达100％；免疫对比试验表明，鸽新城疫油乳剂苗对鸽新城疫病毒的攻毒保护率高于鸡新城疫油乳剂苗；免疫3周后抗体水平达到高峰；疫苗接种16周后仍具有较高的抗体水平；大量的田间试用，效果理想。     

黄孢原毛平革菌处理玉米秸秆的腐解特征

摘要：采用黄孢原毛平革菌处理不同粒度的玉米秸秆,测定了秸秆降解率、木质素降解率、有机质、总腐殖酸、H/F、羧基和酚羟基含量,探讨了秸秆处理过程中的腐解特征。结果表明,在秸秆腐解过程中,腐解物的质量、有机质、木质素含量逐渐降低,其分解率和分解时间与秸秆粒度具有相关性;H/F总体呈上升趋势,腐殖化程度升高;酚羟基含量逐渐降低,羧基含量逐渐升高。这些现象表明秸秆的腐解过程为氧化过程,酚羟基被氧化为羧基。     

嫩江大堤植物根系固土护堤功能研究

通过对嫩江大堤护坡植物根系及土壤抗冲,抗蚀及抗剪强度的测定分析,发现同一地段土壤的冲击,抗蚀指数及抗剪强度的是表土层大于底土层。植物根系有较强的固持土壤功能,抗冲指数与根长,根量间有明显的相关性,单相关系数分别为０．９１４６和０．８１９７。有机质含量高的土壤抗蚀性强,水稳性指数大。水稳性指数与有机质含量间的相关系数０．９１３２,与根长和根量的相关性相对较低,单相关系数分别为０．８３１７和０．８０９     

基于Meta分析的猪后腿性状QTL整合定位

【目的】通过Meta分析,利用数学模型整合与优化猪后腿腿臀质量、腿臀肉质量和腿臀比性状的QTL,提高QTL定位的准确度和有效性,为猪后腿性状QTL的精细定位和分子辅助育种奠定基础。【方法】收集猪后腿腿臀质量,腿臀肉质量和腿臀比性状的QTL及其相关信息,利用BioMercator2.1,将原始QTL映射到美国肉畜研究中心（USDA-MARC 2.0）公布的猪遗传连锁图谱,构建新的整合图谱,分析得到QTL簇。进一步对各QTL簇进行Meta分析,定位“真实”QTL（MQTL）,缩短95%置信区间,减少定位误差。【结果】收集了93个猪后腿性状的QTL及其相关信息,经比对、映射,构建了新的整合图谱,发现19个QTL簇。通过Meta分析,得到19个MQTL,其图距比原平均图距缩短16.19%～78.96%,其中,MQTL1、MQTL5、MQTL6、MQTL8、MQTL9、MQTL10、MQTL11、MQTL12和MQTL17等9个MQTL图距的缩短比例均超过50%。【结论】Meta分析得到的MQTL图距均有不同程度缩短,最小的仅1.75 cM,缩短比例最大可达78.96%,提高了QTL定位的准确度和有效性。     

20万只蛋鸡场计算机辅助管理系统的研制(二)

我们利用汉化FoxBASE2.00版,在IBM-PC/XT微机上,研制成集约化鸡场生产统计处理关系型数据库,实现微机对蛋鸡生产的管理。把养鸡生产中所积累的资料,按鸡场日报文件,建立雏鸡、青年鸡和产蛋鸡每日一库,逐日汇总,形成一个月、季、年和期报文件。已对1987年种鸡场、蛋鸡场、育成场和四个蛋鸡分场的全部生产资料,输入、汇总与打印。并于1991年进入电脑管理与人工管理的同步运行阶段。利用dBASEⅢ和编译BASIC软件,或FoxBASE2·00版,对鸡群合理周转,生产性能指标,计划执行,以及饲料加工出入库,成本等信息进行分析与处理,可方便清晰地获得12份报表,供蛋鸡生产分析用。可提高蛋鸡生产经营管理水平。     

基于RIL群体鉴定棉花抗黄萎病相关QTLs

【目的】鉴定出能够稳定表达的棉花抗黄萎病相关数量性状位点(Quantitative trait loci,QTLs)。【方法】以抗落叶型黄萎病棉花品种常抗棉和感黄萎病品种TM-1为亲本配制的111个重组自交系家系为作图群体,筛选出多态性简单序列重复(Simple sequence repeat,SSR)标记,并用于构建遗传图谱。用完备复合区间作图法对该群体在安阳大田、新疆重病地及病圃等多个环境下的黄萎病病情指数进行QTLs检测。【结果】构建了1张含有12个连锁群、40个标记、总长212.5 cM(厘摩)的遗传图谱。获得了6个与抗黄萎病基因相关的QTLs,对数优势比(Logarithm of the odd score,LOD)分布在2.51～5.55,贡献率最大为20.34%,最小为6.93%。其中,qVR-D05-1能够在安阳大田2015年7月15日和新疆南疆重病地2016年7月9日2个环境中检测到,贡献率分别为12.96%和20.34%。【结论】本研究得到的qVR-D05-1能够为定位出稳定的棉花抗黄萎病相关QTLs提供参考。