阿月浑子砧本研究进展

综合介绍了世界阿月浑子砧木资源、自然分布、栽培表现、育苗及繁殖技术及其研究进展。     

鸡Toll样受体在IBDV免疫中的作用研究

为了揭示鸡先天性免疫反应在IBDV疫苗过程中的作用,将40只14日海兰褐蛋鸡随机分成两组,实验组接种IBDV活疫苗,对照组注射生理盐水,隔离饲养。于免疫前和免疫后12h、24h、48h、72h、130h各取脾脏和法氏囊分离淋巴细胞并提取总RNA,用实时荧光定量检测方法检测各组中chTLR3、chTLR7、chTLR21表达的动态变化。结果显示,在免疫后36h,鸡法氏囊和脾脏中chTLR3表达变化最显著,在免疫后48h,鸡脾脏和法氏囊中chTLR21表达变化也较为明显。提示chTLR3在鸡对IBDV的免疫应答中起主要作用。     

猪FSHβ和GDF9基因中Alu插入序列多态性

使用聚合酶链式反应（Polymerase chain reaction,PCR）技术,对辽宁黑猪、长白猪、大约克猪、丹育猪和杜洛克猪的FSHβ亚基基因和GDF9基因的插入片段扩增后进行多态性和生物信息学分析,并对产仔数建立GLM模型,探讨2个基因插入序列Alu的多态性与猪产仔数的关系。研究表明：优势基因型BBDD纯合子比AACC型纯合子母猪平均每胎多产仔1.5头（P〈0.01）。研究结果表明,FSHβ亚基基因和GDF9基因与控制猪产仔数的主效基因密切相关,这可能会作为猪产仔数性状的遗传标记,而其优势基因型BBDD可以进一步改良猪的产仔数性状。     

犬膀胱尿道结石的诊治

在宠物疾病的临床诊疗中。犬尿结石症是我们经常遇到的一种严重的泌尿系统疾病,其对犬的健康危害严重。引起了兽医工作者的广泛关注。本文根据对泰州爱牧宠物保健中心2004年-2007年门诊尿结石症病例的调查、研究,分析了该地区犬尿结石症的发病率、发病原因等因素,探讨了犬尿结石症的种类、发病机理、临床特征及有效治疗措施,并对犬尿结石症的有效控制和科学预防提出了建议,以期为临床实践提供依据。     

Pancytopenia in a cat with visceral leishmaniasis

Abstract: A 4‐year‐old, domestic shorthair, female spayed cat was presented for decreased appetite and depression. Severe pancytopenia with erythrocyte autoagglutination was found. The cat was seronegative for feline immunodeficiency and leukemia viruses. Immune‐mediated hemolytic anemia was suspected but no response to treatment with a blood transfusion, enrofloxacin, and prednisone was observed. Blood and bone marrow smears obtained 11 days later contained Leishmania amastigotes in the cytoplasm of neutrophils and macrophages, respectively. Serologic and PCR testing of peripheral blood confirmed infection with Leishmania infantum. Despite treatment, the cat worsened clinically and was euthanized. At necropsy, visceral dissemination of the parasite was confirmed. The findings in this case indicate that visceral leishmaniasis should be considered as a differential diagnoses in cats with pancytopenia in areas endemic for Leishmania. In addition, amastigotes may be observed in peripheral blood neutrophils.     

东北地区外来针叶树种引种现状及发展策略

介绍了东北林区主要针叶树种引种现状及取得的成就，分析了东北三省针叶树种引种工作中存在的问题，提出了建立较为科学的引种网络体系和发展策略，并提供了可能在干旱、半干旱区引种成功的针叶树种名     

基于TDLAS的储粮二氧化碳激光检测系统研制

储粮过程由微生物及害虫等组成的生物场与温度和湿度等构成的非生物场所造成的影响会降低粮食品质,影响粮食安全。储粮中二氧化碳浓度反应粮库生物场状况,是储粮生物场监测的重要指标。为实现储粮二氧化碳浓度高精度检测,该研究基于可调谐半导体激光吸收光谱(Tunable Diode Laser Absorption Spectroscopy,TDLAS)技术高灵敏度和快速响应的特点研制了一套储粮二氧化碳浓度现场检测系统,并建立了二氧化碳激光光谱分析与数据处理方法。基于便携式要求,系统采用紧凑型设计,集二氧化碳浓度、温度及湿度检测功能于一体,同时实现弱信号调理、二氧化碳浓度计算及生成多参数云图、水势图显示等功能。整机尺寸338 mm×251 mm×88 mm,室温条件下功耗小于1.3 W。试验结果显示：该检测系统能够长时间稳定工作,人机交互功能鲁棒性较好,对储粮二氧化碳浓度检测的响应时间小于20 s,线性误差小于1.2%,检测重复性为0.4%,检测精度为10mg/m³,检测范围为0～20 000 mg/m³,温度的检测范围为-40～120℃,湿度检测范围为0...     

三门峡烤烟主要化学成分的变异分析

以三门峡烟区4个县市(卢氏、灵宝、渑池、陕县)、3个等级(B2F、C3F、X2F)共计94个烤烟样本为供试材料,对烟叶总糖、还原糖、烟碱、钾、氯、糖碱比、钾氯比等主要化学成分进行了变异分析。结果表明:除钾含量、钾氯比之外,烤烟其他化学成分在不同生产地点之间差异显著;除还原糖、钾、氯含量和钾氯比之外,烤烟其他化学成分不同品种之间差异显著;总糖、钾含量在不同等级之间差异显著;不同海拔的总糖、烟碱和糖碱比差异显著;在不同施氮水平下,还原糖、烟碱和糖碱比差异显著,其余指标差异不显著。     

In vitro plantlet formation from embryonic explants of eastern white cedar (Thuja occidentalis L.)

A protocol is described for the in vitro production of plantlets from embryonic explants of eastern white cedar (Thuja occidentalis L.). Bud induction was optimal when embryonic explants were cultured for 20-25 days on half-strength Quoirin and Le Poivre mineral salts containing equimolar concentrations (10(-6) M) of N(6)-benzyladenine and 2-isopentyl adenine. Bud development was achieved in phytohormone-free medium in the presence of activated charcoal. Maximal shoot elongation occurred on half-strength Quoirin and Le Poivre salts, whereas shoot multiplication was optimal on half-strength Bornman's MCM salts in the presence of cytokinin. Hardened shoots, dipped in commercial rooting powder containing indole-3-butyric acid, rooted optimally in mist under non-sterile greenhouse conditions. Both rooting and subsequent plantlet growth was best when Redi-Earth((R)) was used as a substrate. Over 250 plantlets per embryo can be produced annually by this technique.