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11.
R. James N. Summerfield J. Loureiro S. Swift J. Dukes‐McEwan 《The Journal of small animal practice》2008,49(11):564-570
Objective : The purpose of this study was to investigate whether implantable loop recorders could be used in the diagnosis of unexplained collapse in dogs. Methods : The medical records of six dogs presented to the University of Liverpool Small Animal Teaching Hospital between May 2003 and October 2006 for further evaluation of intermittent syncopal episodes, collapse or episodic weakness, were reviewed. All these dogs underwent standard investigations and had implantable loop recorders placed. Results : A provisional diagnosis of supraventricular tachycardia was made in one dog, and diagnoses of exclusion of arrhythmogenic right ventricular cardiomyopathy and idiopathic epilepsy was made in two dogs. One dog suffered no further syncopal episodes, a diagnosis was not reached in another dog and the final dog was lost to follow‐up. Clinical Significance : The implantable loop recorder can be used successfully for the diagnosis of unexplained collapse in dogs. 相似文献
12.
Conceio V. Santos Inês P. Falco Glria C. Pinto Helena Oliveira Joo Loureiro 《植物养料与土壤学杂志》2002,165(3):366-372
The growth of Helianthus annuus L. calli and plants was reduced in the presence of Na2SO4 (10, 25, 50, and 100 mM). SO42— and Na concentrations increased in stressed calli and plants while NO3—, Cl—, P, K, and Mg decreased in stressed plants and Ca in shoots. Stressed calli showed decreases of NO3—, Ca, K, and Mg concentrations. Calli adapted to 50 mM Na2SO4 accumulated more K and Ca and less ammonium than stressed non‐adapted calli. Proline exhibited increases in stressed calli and plants that were accompanied by decreases of proline oxidase activities while pyrroline‐5‐carboxylate reductase (P5CR) and ornithine aminotransferase (OAT) activities increased. Adapted calli accumulated more proline and had higher P5CR and OAT activities than stressed non‐adapted calli. Glutamate concentration decreased with stress, together with a stimulation of cytosolic glutamine synthetase (GS1) and a decrease of plastidal GS (GS2) activity. These data strongly suggest that the increase of P5CR and GS1 activities are responsible for the decrease of glutamate concentration leading, together with the stimulation of OAT and the inhibition of the proline oxidation metabolism, to an increase of proline levels in Na2SO4‐stressed sunflower cells. These data also show that salt stress increases the release of endogenous ammonium and suggests that the increase of GS1 activity plays an important role in its elimination. 相似文献
13.
E. Raffan J. Loureiro J. Dukes-McEwan S. Fonfara R. James S. Swift N. Bexfield M.E. Herrtage J. Archer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(6):1184-1189
Background: Amino‐terminal probrain natriuretic peptide (NT‐proBNP) has been proposed as a useful biomarker for heart disease in dogs. In humans, decreased glomerular filtration rate (GFR) increases NT‐proBNP. Objective: To investigate whether decreased GFR as indicated by plasma creatinine concentration is associated with increased NT‐proBNP in dogs without heart disease. Animals: Four groups of dogs: healthy (n= 39), azotemic (n= 36), heart disease (n= 37), and congestive heart failure (CHF) (n= 7) presented to 2 teaching hospitals. Methods: Prospective observational cohort study. Plasma creatinine concentration and NT‐proBNP were measured in every dog. Nonparametric tests were used to compare the differences among groups. The median and actual results for each group were compared with the manufacturer's recommended and previously published suggestions for cut‐off values for diagnosis of heart disease. Results: Median (range) plasma creatinine concentration was 1.47 (1.06–1.70), 4.36 (1.74–15.6), 1.22 (0.69–1.91), and 1.45 (0.63–1.64) mg/dL and median (range) NT‐proBNP was 118 (2–673), 556 (37–1,819), 929 (212–5,658), and 3,144 (432–5,500) pmol/L for the healthy, azotemic, heart disease, and CHF groups, respectively. Pair‐wise comparison indicated a significant difference among all groups for NT‐proBNP (P≤ .049). Plasma creatinine concentration was significantly higher in the azotemic group compared with other groups (P < .001) but there was no significant among other groups. Application of 3 recommended cut‐off values led to misclassification of dogs with azotemia as having heart disease. Conclusions: Azotemia results in NT‐proBNP being increased to concentrations reported as diagnostic of heart disease or heart failure in dogs. Care should be employed when interpreting the results of NT‐proBNP in patients with known or possible increased plasma creatinine concentration. 相似文献
14.
Goldman CG Loureiro JD Matteo MJ Catalano M Gonzalez AB Heredia SR Zubillaga MB Solnick JV Cremaschi GA 《Research in veterinary science》2009,86(1):18-21
Gastrointestinal lesions with uncertain etiology have been widely described among pinnipeds. The aim of our study was to investigate the presence of Helicobacter spp. in the gastric mucosa of South American fur seals (Arctocephalusaustralis). Gastric biopsies from thirteen seals, stranded on the shores of the Southwestern Atlantic Ocean in Argentina, were evaluated for the presence of Helicobacter spp. by PCR and DNA sequence analysis. Six gastric biopsies were positive for Helicobacter spp. Pairwise sequence comparisons showed less than 95% identity to novel Helicobacter spp. described from pinnipeds from North America and Australia. However, phylogenetic analysis revealed that the South American fur seal sequences clustered with 99-100% homology with H. cetorum, a species isolated from dolphins and whales. The presence of H. cetorum in pinnipeds, if confirmed by its isolation from the gastric mucosa of these mammals, demonstrates the wide host range of this bacterium in the marine environment. 相似文献
15.
J. Obidiegwu J. Loureiro E. Ene-Obong E. Rodriguez M. Kolesnikova-Allen C. Santos C. Muoneke R. Asiedu 《Euphytica》2009,169(3):319-326
The Guinea yams, Dioscorea
cayenensis Lam. and D. rotundata Poir. (D. cayenensis–D. rotundata complex), represent a highly important crop, widely distributed in the humid and semi-humid tropics. The ploidy levels of
170 accessions of the core set of Guinea yams from West African countries was determined using flow cytometry with propidium
iodide staining. One hundred and eight of the genotypes were found to be tetraploid, 47 were hexaploid and five were octoploid.
One mixoploid individual containing tetraploid and hexaploid nuclei was also detected. A deeper analysis considering each
separate taxon revealed that while for D. rotundata the majority of individuals were tetraploid, for D. cayenensis this ploidy level was not detected in any of the accessions. Also, no association between ploidy level and place of cultivation
was found for the evaluated germplasm. The obtained data is highly valuable for breeding programs of Guinea yam, especially
for the optimization of future hybridization experiments directed to the genetic improvement of this economically important
crop. 相似文献
16.
Giovani Greigh de Brito Eveline Teixeira Caixeta Ana Paula Gallina Eunize Maciel Zambolim Laércio Zambolim Valdir Diola Marcelo Elhers Loureiro 《Euphytica》2010,173(2):255-264
The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and
to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and
the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido
de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations
using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene.
Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and
E.CGT/M.TGT300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while
E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance
markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs. 相似文献
17.
El-Warrak AO Ferrer GA Lanthier T Loureiro Silva B El-Warrak LO 《The Journal of small animal practice》2011,52(3):158-162
Objective : To determine the effect of unilateral condylectomy on dental occlusion in cats. Methods : Twelve feline cadaver heads were randomly submitted to either a right or left unilateral condylectomy of the temporo‐mandibular joint. The distance between the mandibular and maxillary canine tooth was measured before (B0) and after the unilateral condylectomy (P0). A right or left latero‐‐lateral standardised force (4 N) was applied to the mandibular mentus area before and after the surgical procedure. These measurements were analysed with a linear model for repeated measures. Results : The comparative analysis between pre‐ and postsurgical values indicated no significant variation in teeth displacement following condylectomy between B0 and P0 measurements. Statistically significant differences were detected after either left or right condylectomy with respect to all measurements after application of the standardised forces. Occlusion changes are significant when comparing bites before and after surgery. Clinical Significance : Unilateral condylectomy causes a significant increase in latero‐lateral amplitude of jaw movement which might clinically affect feline dental occlusion. Clinical studies are required to determine the effect of unilateral condylectomies on mastication and dental occlusion in feline patients. 相似文献
18.
Effect of resistant starch on the intestinal health of old dogs: fermentation products and histological features of the intestinal mucosa 下载免费PDF全文
M. C. Peixoto É. M. Ribeiro A. P. J. Maria B. A. Loureiro L. G. di Santo T. C. Putarov F. N. Yoshitoshi G. T. Pereira L. R. M. Sá A. C. Carciofi 《Journal of animal physiology and animal nutrition》2018,102(1):e111-e121
The effects of resistant starch (RS) intake on nutrient digestibility, microbial fermentation products, faecal IgA, faecal pH, and histological features of the intestinal mucosa of old dogs were evaluated. The same formulation was extruded in two different conditions: one to obtain elevated starch cooking degree with low RS content (0.21%) and the other lower starch cooking with high RS content (1.46%). Eight geriatric Beagles (11.5 ± 0.38 years old) were fed each diet for 61 days in a crossover design. Food intake, nutrient digestibility, fermentation products, faecal pH, and faecal IgA were examined via variance analysis. Histological results of intestinal biopsies were assessed via Wilcoxon test for paired data. The morphometric characteristics of large intestine crypts were evaluated via paired t tests (p < .05). Protein, fat, and energy digestibilities were higher for the low‐RS diet (p < .05). Dogs receiving the high‐RS diet had lower faecal pH and higher values for propionate, butyrate, total volatile fatty acids, and lactate (p < .05). No differences between diets were found in the histological parameters of the gut mucosa, and only a tendency for deeper crypts in the descending colon was observed for dogs fed the high‐RS diet (p = .083). The intake of a corn‐based kibble diet manufactured with coarse ground raw material and low starch gelatinization to obtain 1.4% of RS affected microbial fermentation products and faecal pH and tended to increase crypt depth in the descending colon of old dogs. 相似文献
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J. Novo Matos N. Pereira T. Glaus L. Wilkie K. Borgeat J. Loureiro J. Silva V. Law A. Kranjc D.J. Connolly V. Luis Fuentes 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):48-56