Global and Phylogenetic Distribution of Quorum Sensing Signals,Acyl Homoserine Lactones,in the Family of Vibrionaceae

Bacterial quorum sensing (QS) and the corresponding signals, acyl homoserine lactones (AHLs), were first described for a luminescent Vibrio species. Since then, detailed knowledge has been gained on the functional level of QS; however, the abundance of AHLs in the family of Vibrionaceae in the environment has remained unclear. Three hundred and one Vibrionaceae strains were collected on a global research cruise and the prevalence and profile of AHL signals in this global collection were determined. AHLs were detected in 32 of the 301 strains using Agrobacterium tumefaciens and Chromobacterium violaceum reporter strains. Ethyl acetate extracts of the cultures were analysed by ultra-high performance liquid chromatography-high resolution mass spectrometry (MS) with automated tandem MS confirmation for AHLs. N-(3-hydroxy-hexanoyl) (OH-C6) and N-(3-hydroxy-decanoyl) (OH-C10) homoserine lactones were the most common AHLs found in 17 and 12 strains, respectively. Several strains produced a diversity of different AHLs, including N-heptanoyl (C7) HL. AHL-producing Vibrionaceae were found in polar, temperate and tropical waters. The AHL profiles correlated with strain phylogeny based on gene sequence homology, however not with geographical location. In conclusion, a wide range of AHL signals are produced by a number of clades in the Vibrionaceae family and these results will allow future investigations of inter- and intra-species interactions within this cosmopolitan family of marine bacteria.     

大数据时代农村信息服务的实例研究

大数据时代,建立农村信息服务体系对促进农村经济发展和提高农民收入,加快农业现代化,推动农村城市化意义非常重要。本文分析了北京地区北臧村镇农业信息服务的工作现状,揭示了存在的问题,采用李克特量表和农村信息服务综合评价指标体系进行调研和量化分析,并针对农业信息服务提出了改进建议。     

露地番茄品种比较试验

引进12个番茄品种并对其丰产性、商品性和适口性进行比较试验。试验结果表明：在参试的12个品种中,综合性状最好的是‘毛粉812’,其次是‘春秋粉宝’,表现丰产、整齐度高、果形端正等,适合本地区栽植,有很好的推广价值,应加大推广力度;‘新选906’为自封顶类型,商品果小,产量低且综合性状差,不适合在春季露地栽培。     

Monitoring distances travelled by horses using GPS tracking collars

Objective The aims of this work were to (1) develop a low-cost equine movement tracking collar based on readily available components, (2) conduct preliminary studies assessing the effects of both paddock size and internal fence design on the movements of domestic horses, with and without foals at foot, and (3) describe distances moved by mares and their foals. Additional monitoring of free-ranging feral horses was conducted to allow preliminary comparisons with the movement of confined domestic horses. Procedures A lightweight global positioning system (GPS) data logger modified from a personal/vehicle tracker and mounted on a collar was used to monitor the movement of domestic horses in a range of paddock sizes and internal fence designs for 6.5-day periods. Results In the paddocks used (0.8–16 ha), groups of domestic horses exhibited a logarithmic response in mean daily distance travelled as a function of increasing paddock size, tending asymptotically towards approximately 7.5 km/day. The distance moved by newborn foals was similar to their dams, with total distance travelled also dependent on paddock size. Without altering available paddock area, paddock design, with the exception of a spiral design, did not significantly affect mean daily distance travelled. Feral horses (17.9 km/day) travelled substantially greater mean daily distances than domestic horses (7.2 km/day in 16-ha paddock), even when allowing for larger paddock size. Conclusions Horses kept in stables or small yards and paddocks are quite sedentary in comparison with their feral relatives. For a given paddock area, most designs did not significantly affect mean daily distance travelled.     

QUANTIFICATION OF CEREBRAL VENTRICULAR VOLUME IN ENGLISH BULLDOGS

Quantitative measurement of cerebral ventricle volume of eight English bulldogs was performed using magnetic resonance (MR) imaging. The mean ventricular volume was 14.8 ml. with a range of 8.6 m1.-38.1 ml. The mean ventricular volume of two beagles was 2.2 ml with a range of 0.7 m1.3.7 ml. The percent of intracranial volume occupied by ventricle was found to be significantly larger in bulldogs (14.0%; S.D. = 7.9%) than in beagles (Range = 1.048%). The relationship between the percent of intracranial volume occupied by ventricle and measurements of body weight, age, sex, and various measures of skull anatomy of the bulldog was also determined. The relationship between ventricular volume and neurologic dysfunction was examined. There was a possible trend between high percent of intracranial volume occupied by ventricle and low body weight. This study will serve as a pilot study for examining the relationship between ventricular volume and neurologic disease in bulldogs.     

地下水埋深对春玉米田土壤水分及产量的影响

通过6种地下水位控制处理和对照春玉米试验,探讨了地下水埋深对春玉米农田土壤水分、产量以及水分利用效率的影响。结果表明,地下水埋深对春玉米0～100cm层次土壤水分的影响较大。地下水位越浅这种影响显得越明显;无论地下水埋深深浅,田间累计地下水补给量变化规律可分为4个阶段,即稳定增长期、缓慢增长期、快速增长期和趋于稳定期。地下水位越高,累计地下水补给量越大,土壤排水量越大。较大降雨后土壤开始排水日期随着地下水埋深加深而滞后;地下水埋深1.0m时春玉米产量和水分利用率最高。地下水位在1.0m以下时,水分利用效率随地下水位加深而减少。     

重齿小蠹聚集信息素诱捕器的应用技术研究

重齿小蠹对人工合成聚集信息素的昼夜反应节律发生在白天7：00～21：00,反应高峰在14：00-19：00,反应模式为＂单峰式—白天型＂;利用人工合成的聚集信息素对重齿小蠹成虫发生期监测表明,成虫扬飞的开始时间为5月20日左右,扬飞高峰期为6月下旬和7月上旬,扬飞末期为8月26日,成虫扬飞的持续期为98 d。2007-2009年大量诱杀防治的试验前、后重齿小蠹致死木平均分别为3.39株/hm2和0.76株/hm2,致死木减少了77.58%,而对照区试验前、后致死木平均分别为15.89株/hm2和18.11株/hm2,致死木增加了13.97%,3 a大量诱杀试验的防治效果显著。