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101.
Land-use and vegetation cover have been linked to the nutrient levels (nitrogen, phosphorus) of surface waters in several
countries. However, the links generally relate to streams and rivers, or to specific types of standing water, for example
shallow lakes in a geologically defined region. We measured physical variables and nutrient chemistry of 45 water bodies representative
of the wide range of lentic wetland environments (swamps, riverine wetlands, estuaries, reservoirs, shallow lakes, deep lakes)
in Otago, New Zealand, and related these to catchment variables and land-use in order to assess the potential influence of
catchment modification on water quality of these diverse wetlands. Catchment boundaries and land cover were derived from maps
using ArcView GIS software. Our predictions that concentrations of nutrients and other components of water quality would correlate
positively with the nature and intensity of catchment modification were confirmed in multivariate analyses. Physical and chemical
measures were positively related to the extent of modification in the catchment (percentage of the catchment in pasture, planted
forest, scrub and urban areas), and negatively related to lack of catchment modification (more of the catchment in bare ground,
tussock grassland and indigenous forest). The strong negative correlations between nutrient concentrations, suspended sediment,
water colour and the percentage of tussock cover in the catchment imply that increased conversion of the␣native tussock grassland
to pastoral farming in␣Otago will increase nutrient concentrations and␣reduce water quality of the diverse lentic ecosystems. 相似文献
102.
A significant challenge facing forestry today is managing private forests sustainably in the face of continued ownership fragmentation
(i.e., parcelization). Cross-boundary coordination––where forest practices are coordinated across multiple properties––has
been proposed as a mechanism by which landscape-level ecological and economic benefits may be accrued in privately-owned landscapes,
but few tests of the concept exist. Using a case study approach, we quantify the extent to which ownership-centric forest
management is constrained by economies of scale and misses opportunities to achieve ecological objectives in three landscapes
in Wisconsin, USA. Methods are based on existing forest management plans and include spatial analysis of patch distributions
and shapes, simulation of forest practices, and calculation of net present value over a 20-year horizon. Our results indicate
substantial opportunity for cross-boundary coordination: between 62% and 88% of the managed properties within our study landscapes
were adjacent to other properties with forest management plans. At a patch scale, coordination can result in ecological benefits
that can be accrued into the future (e.g., maintenance of large patches and natural ecosystem boundaries). Because these landscapes
are already highly parcelized, however, coordination offers little opportunity to impact the overall landscape-scale structure.
Greater economies of scale can also be gained by coordinating forest practices, including increases in the size (16–99%) and
volume of timber sales (16–94%), and a modest economic advantage (3–6%). As first steps, investment in data infrastructure
and professional training are required to support cross-boundary multi-ownership forest management. More broadly is the need
to shift from policies and practices that are largely ownership-centric to those that include and better incorporate landscape-centric
perspectives. 相似文献
103.
Puccinia triticina f. sp. tritici, the causal agent of leaf rust causes yield losses in wheat up to 60%. In order to avoid such losses, leaf rust resistance (Lr-) genes have been incorporated into wheat cultivars. The Lr- genes confer mostly vertical resistance, i.e. they are race specific. Therefore, knowledge of still effective resistance genes is required for efficient breeding of resistant cultivars. To get information on these virulences, a leaf rust population was monitored in field experiments in 2010. For this purpose naturally infection at three different timepoints of wheat development was monitored on Thatcher near isogenic lines (NILs) carrying 37 and accessions carrying 6 additional Lr-genes. Thatcher-NILs carrying Lr2a, Lr9, Lr19, Lr22a, Lr23, Lr24, Lr35, Lr38 and Lr49 showed a significantly lower infection with Puccinia triticina than the susceptible cultivar Thatcher. Thatcher-NILs carrying Lr13, Lr16, Lr37 and Lr46 showed no significant differences in comparison to Thatcher. In order to get information on the effectiveness of resistance genes, P. triticina isolates were collected from the NILs analysed in field trials and a leaf segment test was conducted followed by microscopic analyses. In the field and in the leaf segment test Lr9, Lr19, Lr24 and Lr38 and to some extent Lr3a turned out to be the most effective genes. By microscopic analyses, the infection process as well defense reactions activated before macroscopic symptoms are visible were monitored. By counting haustorial mother cells, it could be demonstrated which Lr-genes provide resistance, which were overcame and whether P. triticina isolates exist already at a low frequency, which may overcome a certain Lr-gene in the future. Thus microscopy offers a timesaving and effective method to detect susceptible or resistant plants and the upcoming of virulent races prior to typical symptom expression. 相似文献
104.
105.
Lisa S Baller Dean A Hendrickson 《Veterinary Clinics of North America: Equine Practice》2002,18(1):117-31, vii
Pain management is an extremely vital part of equine orthopedic surgery. Providing optimum analgesia for the patient will decrease recovery time, decrease physiologic stress on the animal, and provide maximum comfort during the post-operative period. The major analgesic drug categories and routes covered are nonsteroidal anti-inflammatory drugs, local anesthetics, intra-articular drugs, and epidurals. 相似文献
106.
Arnhold SJ Goletz I Klein H Stumpf G Beluche LA Rohde C Addicks K Litzke LF 《American journal of veterinary research》2007,68(10):1095-1105
OBJECTIVE: To isolate and characterize bone marrow-derived equine mesenchymal stem cells (MSCs) for possible future therapeutic applications in horses. SAMPLE POPULATION: Equine MSCs were isolated from bone marrow aspirates obtained from the sternum of 30 donor horses. PROCEDURES: Cells were cultured in medium (alpha-minimum essential medium) with a fetal calf serum content of 20%. Equine MSC features were analyzed to determine selfrenewing and differentiation capacity. For potential therapeutic applications, the migratory potential of equine MSCs was determined. An adenoviral vector was used to determine the transduction rate of equine MSCs. RESULTS: Equine MSCs can be culture-expanded. Equine MSCs undergo cryopreservation in liquid nitrogen without altering morphologic characteristics. Furthermore, equine MSCs maintain their ability to proliferate and differentiate after thawing. Immunocytochemically, the expression of the stem cell marker CD90 can be detected on equine MSCs. The multilineage differentiation potential of equine MSCs was revealed by their ability to undergo adipogenic, osteogenic, and chondrogenic differentiation. CONCLUSIONS AND CLINICAL RELEVANCE: Our data indicate that bone marrow-derived stromal cells of horses can be characterized as MSCs. Equine MSCs have a high transduction rate and migratory potential and adapt to scaffold material in culture. As an autologous cell population, equine MSCs can be regarded as a promising cell population for tissue engineering in lesions of the musculoskeletal system in horses. 相似文献
107.
Jinhe Bai Eugene A. Mielke Paul M. Chen Robert A. Spotts Maryna Serdani James D. Hansen Lisa G. Neven 《Postharvest Biology and Technology》2006,40(3):207-215
A manually operated high-pressure hot-water washing system consisting of a boiler, hot-water mixing tank, contact loop, heat exchanger, spray mixing tank, high-pressure hot-water washing manifold, low-pressure fresh water rinse manifold, and pressure pump was constructed and installed in a packingline. The system developed 20–50 °C washing water at pressures up to 980 kPa. ‘d’Anjou’ pears (Pyrus communis L.), shortly after harvest, and after storage for 3 and 4 months in regular air (RA) or for 4, 7 and 8 months in controlled atmosphere (CA) at −1 °C were washed through the packingline with different wetting agents (0.1% Silwet, 0.01 and 0.1% Defoamer, and water), water pressures (regular and high-pressure (210–980 kPa)), water temperatures (control (tap water, 4–22 °C), 40 °C, and 50 °C), and brushes (soft and firm), respectively. The effect of the washing conditions on fruit quality was investigated after 1 month of storage at −1 °C to simulate shipping condition, and then again after 1 week at 20 °C to simulate marketing condition. Hot-water caused severe heat scald. When nozzle temperature was 50 °C, the incidence of heat scald increased to over 50% for the fruit stored in RA for 3 months. Combined with hot-water, 540 kPa high-pressure washing increased the incidence of friction discoloration. There were lower incidences of friction discoloration and heat scald for fruit stored in CA for 7 months, in comparison to that in RA for 3 months. However, those fruit did not ripen properly as indicated by a high extractable juice content. Fruit washed at harvest had minor incidences of friction discoloration regardless of different brushes, water pressures, and wetting agents. Fruit washed after storages in either 4 months RA or 4 or 8 months CA suffered a high incidence of friction discoloration including scuffing symptoms and pressure marking. The firm brushes caused a higher incidence of friction discoloration mainly because of scuffing symptoms. However, no differences were found between different water pressures and wetting agents with respect to friction discoloration. Fruit stored for 4 months RA suffered 26–28% friction discoloration in comparison to 16–18% in CA stored fruit with firm brush washing. Extended CA storage increased friction discoloration even with soft brush washing. The results suggest that a washing system with high-pressure spray, <30 °C warm water, wetting agent Defoamer and rotating soft brushes were significantly effective in removing surface pests and decay control without causing internal or external damage of fruit. 相似文献
108.
Cifuentes L Borja-Aburto VH Gouveia N Thurston G Davis DL 《Science (New York, N.Y.)》2001,293(5533):1257-1259
109.
Steed PM Tansey MG Zalevsky J Zhukovsky EA Desjarlais JR Szymkowski DE Abbott C Carmichael D Chan C Cherry L Cheung P Chirino AJ Chung HH Doberstein SK Eivazi A Filikov AV Gao SX Hubert RS Hwang M Hyun L Kashi S Kim A Kim E Kung J Martinez SP Muchhal US Nguyen DH O'Brien C O'Keefe D Singer K Vafa O Vielmetter J Yoder SC Dahiyat BI 《Science (New York, N.Y.)》2003,301(5641):1895-1898
Tumor necrosis factor (TNF) is a key regulator of inflammatory responses and has been implicated in many pathological conditions. We used structure-based design to engineer variant TNF proteins that rapidly form heterotrimers with native TNF to give complexes that neither bind to nor stimulate signaling through TNF receptors. Thus, TNF is inactivated by sequestration. Dominant-negative TNFs represent a possible approach to anti-inflammatory biotherapeutics, and experiments in animal models show that the strategy can attenuate TNF-mediated pathology. Similar rational design could be used to engineer inhibitors of additional TNF superfamily cytokines as well as other multimeric ligands. 相似文献
110.