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41.
42.
Schellekens M Hoppeler R Perrin A Gomes JV Boiron D Aspect A Westbrook CI 《Science (New York, N.Y.)》2005,310(5748):648-651
We have studied two-body correlations of atoms in an expanding cloud above and below the Bose-Einstein condensation threshold. The observed correlation function for a thermal cloud shows a bunching behavior, whereas the correlation is flat for a coherent sample. These quantum correlations are the atomic analog of the Hanbury Brown Twiss effect. We observed the effect in three dimensions and studied its dependence on cloud size. 相似文献
43.
44.
Li X Bickerdike R Lindsay E Campbell P Nickell D Dingwall A Johnston IA 《Journal of agricultural and food chemistry》2005,53(17):6844-6850
A simple HPLC method is presented to quantify the low concentration of hydroxylysyl pyridinoline (PYD) cross-links in Atlantic salmon (Salmo salar L.) muscle. The method involved the extraction of tissue with NaOH prior to hydrolysis, which greatly reduced the amount of protein to be hydrolyzed and made downstream operations easier and more reproducible. The concentration of PYD was 426 pmol g(-)(1) dry mass muscle in post-rigor muscle stored at 0 degrees C and sampled 3 d after death. Hydroxproline (HYP) concentration was determined following NaOH extraction as a measure of collagen content. In post-rigor samples, the alkaline-insoluble HYP fraction comprised 18.3% of the total HYP. Scanning electron microscopy revealed shrinkage of muscle fibers and a retraction of the connective tissue matrix in smoked salmon. PYD concentration was relatively resistant to processing to the smoked product, decreasing by around 11.7%, as compared to a 22.2% decrease in HYP. There was a positive correlation between PYD concentration and the firmness of post-rigor muscle samples as measured by an instrumental texture analyzer, explaining 25% of the total variation. A weaker but still significant correlation was found between PYD concentration and firmness in the smoked product. There was no relationship between fillet firmness and total collagen concentration, although the correlation with HYP in the alkaline-insoluble fraction was significant at the 6% level (P = 0.057). Our results indicate that only 1-3% of collagen molecules are linked by nonreducible mature cross-links in harvest size farmed Atlantic salmon and that PYD concentration is an important raw material characteristic for flesh quality. 相似文献
45.
S K Mikota L Peddie J Peddie R Isaza F Dunker G West W Lindsay R S Larsen M D Salman D Chatterjee J Payeur D Whipple C Thoen D S Davis C Sedgwick R J Montali M Ziccardi J Maslow 《Journal of zoo and wildlife medicine》2001,32(1):1-16
The deaths of two Asian elephants (Elephas maximus) in August 1996 led the United States Department of Agriculture to require the testing and treatment of elephants for tuberculosis. From August 1996 to September 1999. Mycobacterium tuberculosis infection was confirmed by culture in 12 of 118 elephants in six herds. Eight diagnoses were made antemortem on the basis of isolation of M. tuberculosis by culture of trunk wash samples; the remainder (including the initial two) were diagnosed postmortem. We present the case histories, epidemiologic characteristics, diagnostic test results, and therapeutic plans from these six herds. The intradermal tuberculin test, enzyme-linked immunosorbent assay serology, the blood tuberculosis test, and nucleic acid amplification and culture are compared as methods to diagnose M. tuberculosis infection in elephants. 相似文献
46.
Global chondrichthyan (shark, ray, skate and chimaera) landings, reported to the United Nations Food and Agriculture Organization (FAO), peaked in 2003 and in the decade since have declined by almost 20%. In the FAO's 2012 ‘State of the World's Fisheries and Aquaculture’ report, the authors ‘hoped’ the reductions in landings were partially due to management implementation rather than population decline. Here, we tested their hypothesis. Post‐peak chondrichthyan landings trajectories from 126 countries were modelled against seven indirect and direct fishing pressure measures and eleven measures of fisheries management performance, while accounting for ecosystem attributes. We found the recent improvement in international or national fisheries management was not yet strong enough to account for the recent decline in chondrichthyan landings. Instead, the landings declines were more closely related to fishing pressure and ecosystem attribute measures. Countries with the greatest declines had high human coastal population sizes or high shark and ray meat exports such as Pakistan, Sri Lanka and Thailand. While important progress had been made, country‐level fisheries management measures did not yet have the strength or coverage to halt overfishing and avert population declines of chondrichthyans. Increased implementation of legally binding operational fisheries management and species‐specific reporting is urgently required to avoid declines and ensure fisheries sustainability and food security. 相似文献
47.
Vemulapalli R Sanakkayala N Gulani J Schurig GG Boyle SM Lindsay DS Sriranganathan N 《Veterinary parasitology》2007,148(3-4):219-230
Neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of bovine neosporosis, an important disease affecting the reproductive performance of cattle worldwide. Currently there is no effective vaccine available to prevent N. caninum infection in cattle. In this study, we examined the feasibility of developing a live, recombinant N. caninum vaccine using Brucella abortus vaccine strain RB51 as the expression and delivery vector. We generated two recombinant RB51 strains each expressing SRS2 (RB51/SRS2) or GRA7 (RB51/GRA7) antigens of N. caninum. BALB/c mice immunized by single intraperitoneal inoculation of the recombinant RB51 strains developed IgG antibodies specific to the respective N. caninum antigen. In vitro stimulation of splenocytes from the vaccinated mice with specific antigen resulted in the production of interferon-gamma, but not IL-5 or IL-10, suggesting the development of a Th1 type immune response. Upon challenge with N. caninum tachyzoites, mice vaccinated with strain RB51/SRS2, but not RB51/GRA7, showed significant resistance to cerebral infection when compared to the RB51 vaccinated mice, as determined by the tissue parasite load using a real-time quantitative TaqMan assay. Interestingly, mice vaccinated with either strain RB51 or RB51/GRA7 also contained significantly lower parasite burden in their brains compared to those inoculated with saline. Mice vaccinated with strain RB51/SRS2 or RB51/GRA7 were protected to the same extent as the strain RB51 vaccinated mice against challenge with B. abortus virulent strain 2308. These results suggest that a recombinant RB51 strain expressing an appropriate protective antigen(s), such as SRS2 of N. caninum, can confer protection against both neosporosis and brucellosis. 相似文献
48.
Levine JM Levine GJ Boozer L Schatzberg SJ Platt SR Kent M Kerwin SC Fosgate GT 《Journal of the American Veterinary Medical Association》2008,232(3):411-417
OBJECTIVE: To determine complications and neurologic outcomes associated with dexamethasone administration to dogs with surgically treated thoracolumbar intervertebral disk herniation, compared with dogs not receiving dexamethasone. DESIGN: Retrospective case series. ANIMALS: 161 dogs with surgically confirmed thoracolumbar disk herniation. PROCEDURES: Medical records from 2 hospitals were used to identify dogs that had received dexamethasone < 48 hours prior to admission (dexamethasone group dogs), dogs that received glucocorticoids other than dexamethasone < 48 hours prior to admission (other-glucocorticoid group dogs), and dogs that received no glucocorticoids (nontreatment group dogs). Signalment, neurologic injury grade, laboratory data, and complications were extracted from medical records. RESULTS: Dexamethasone group dogs were 3.4 times as likely to have a complication, compared with other-glucocorticoid or nontreatment group dogs. Dexamethasone group dogs were 11.4 times as likely to have a urinary tract infection and 3.5 times as likely to have diarrhea, compared with other-glucocorticoid or nontreatment group dogs. No differences in neurologic function at discharge or recheck evaluation were detected among groups. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that treatment with dexamethasone before surgery is associated with more adverse effects, compared with treatment with glucocorticoids other than dexamethasone or no treatment with glucocorticoids, in dogs with thoracolumbar intervertebral disk herniation. In this study population, no difference in outcome was found among groups. These findings suggest that the value of dexamethasone administration before surgery in dogs with thoracolumbar disk herniation should be reconsidered. 相似文献
49.
J P Dubey David S Lindsay 《Veterinary Clinics of North America: Food Animal Practice》2006,22(3):645-671
Neospora caninum, Sarcocystis spp, and Toxoplasma gondii are related coccidian parasites that can cause abortion and neonatal mortality in animals. In addition, T gondii and certain species of Sarcocystis are zoonotic. This article reviews information on the etiology, diagnosis, control, and prevention of these diseases. 相似文献
50.
At least three species of Sarcocystis (S. neurona, S. falcatula, S. speeri) have recently been shown to use opossums of the genus Didelphis as their definitive host. In order to evaluate the evolutionary relationships among Sarcocystis spp. isolates from the Americas, and to determine whether organisms representing the same parasite lineages are transmitted north and south of the Panamanian isthmus, we inferred the phylogenetic relationships from nucleotide sequence variation in parasites isolated from three opossum species (D. virginiana, D. albiventris, D. marsupialis). In particular, we used variation in the 25/396 marker to compare several isolates from Brazil, Argentina, and the United States to each other and to cloned S. neurona and S. falcatula whose morphology and host affinities have been defined in the laboratory. S. neurona was identified from a Brazilian D. albiventris, as well as from North American D. virginiana. Parasites resembling the Cornell isolate of S. falcatula are transmitted both south and north of the Panamanian isthmus by D. albiventris and D. virginiana, respectively. Distinct attributes at two genetic loci differentiated a Brazilian isolate of S. falcatula from all other known parasite lineages. We confirm S. neurona as the causative agent of recently reported neurologic disease in Southern sea otters, Enhydra lutris nereis. And we found that S. speeri could not be compared to the other opossum-derived Sarcocystis isolates on the basis of nucleotide variation at the 25/396 locus. The widespread distribution of certain species of Sarcocystis may derive from their ability to parasitize migratory bird hosts in their intermediate stage. 相似文献