如何选择缉毒犬

实践已证明,在犬群中只有约二十分之一的犬适宜作为缉毒犬,因为本身的自然素质及心理素质存在着一定的差异,犬的潜能也是有区别的.好的受训犬能节省训犬员训练时间,提高训练成功率及训犬员的信心,更好地为人民服务.     

寡糖在反刍动物营养中的作用

寡糖(Oligosaccharides,Os)又称寡聚糖或低聚糖,是指2～10个单糖通过糖苷键连接形成的一类糖,是近年来开发的无公害饲料添加剂之一.由于具有无污染、无残留、功能奇特等优点,日益受到人们的重视,已成为当今研究的热点.在日本,食品和饲料中使用寡糖已成为常规 [1].其他各国饲料中寡糖的使用量也呈逐年上升的趋势.     

牛乳免疫球蛋白和补体含量测定

用琼脂单项免疫扩散法测定牛乳免疫球蛋白和补体含量。结果表明：初乳免疫球蛋白和补体Ｃ３含量极显著高于常乳（Ｐ〈０．０１）;初乳补体Ｃ４含量显著高于常乳（Ｐ〈０．０５）;初乳中免疫球蛋白和补体含量于产后３天达到高峰。     

蚕蛹蛋白水解技术研究进展

目前蚕业资源综合利用正作为一个新兴产业得到广泛关注。蚕蛹是缫丝过程中的副产物,富含蛋白质,含有人体需要的18种氨基酸,其中8种必需氨基酸含量达40%以上,其组成和比例非常符合WHO/FAO提出的氨基酸模式,是优质蛋白质及氨基酸来源。我国每年有10多万吨干蚕蛹可供利用,但大多被     

漳浦县中蜂高产措施

养中蜂最主要的目的是追求蜂蜜产量.目前国内有关中蜂技术较全面、系统的著作有杨冠煌老师的<中华蜜蜂>和龚凫羌先生的<中蜂饲养原理与方法>.但至今尚未见到有相当规模的示范蜂场的报道.我的技术措施在我地推广了20多年,现在已经在多个地区全面开花了. 今年春季的荔枝、龙眼花期,我县平均单脾产量为5～6.5千克,中蜂群产量在20～30千克,单脾平均产量为5.5～7千克.     

山羊鼻内腺瘤和腺癌的病理学研究

发生于内蒙古自治区某地的山羊鼻内肿瘤呈地方性流行，临床上病羊初期流出大量浆液性鼻液，随后逐渐出现呼吸困难和鼻塞音，肿瘤可侵犯周围组织引起眼球突出和头颅变形，最后死于窒息。１４例山羊鼻内肿瘤的病理学研究表明，肿瘤起源于筛骨迷路粘膜的腺体（包括浆液腺和粘液腺），组织学上为腺瘤，以后可转变为腺癌，并向周围组织作浸润性生长，未见转移。２例作透射电镜检查见瘤组织有异型性，瘤组织和瘤组织内未见病毒样颗粒。发生     

Characterization of porcine circovirus type 2 (PCV2) infection in swine lymphocytes using mitogen-stimulated peripheral blood lymphocytes from healthy PCV2-carrier pigs

Information regarding the susceptibility of swine lymphocytes to PCV2 is rather limited. To further explore and characterize the PCV2 infection in swine lymphocytes, an in vitro model using concanavalin A (Con A)-stimulated peripheral blood lymphocytes (PBLs) obtained from clinically healthy PCV2-carrier pigs was introduced. It was found that the PCV2 antigen-containing rate was below 2% in PBLs from healthy PCV2-free pigs following treated simultaneously with Con A and PCV2. However, significantly higher PCV2 antigen- and nucleic acid-containing rates could be seen in Con A-stimulated PBLs from clinically healthy PCV2-carrier pigs. Prior to Con A treatment, both of the PCV2 antigen- and nucleic acid-containing rates in PBLs from healthy PCV2-carrier pigs were less than 1%; however, they reached 22.1+/-5.7% by flow cytometry and 27.1+/-6.5% by in situ hybridization, respectively, at 4-day post-incubation with Con A. Phenotyping of PCV2 antigen-containing cells revealed that PCV2-positive cells could be detected in both T and B lymphocyte populations within which IgM-positive B lymphocytes appeared to have a relatively higher positive rate. The Con A-stimulated PBLs also displayed a significantly higher viral load by the measurement of either PCV2 DNA copy number or viral titer when compared with the non-treated PBLs from healthy PCV2-carrier pigs. The results indicate that PBLs, especially IgM-bearing B lymphocytes, are indeed susceptible to PCV2 infection and PCV2 is capable of replicating in dividing lymphocytes. This activation-induced replication may explain in part the pathogenesis of lymphoid depletion in PMWS-affected pigs.     

基于荧光显色的环介导等温扩增技术(LAMP)检测禽呼肠孤病毒

建立了一种基于荧光显色的禽呼肠孤病毒(ARV)环介导等温扩增(LAMP)检测方法.该检测方法使用针对ARV的p10基因8个区域的6条LAMP引物,能够在等温(63℃)条件下1h内完成反应,具有良好的敏感性和特异性,最低能够检出102个拷贝的病毒,敏感性比普通PCR高100倍,而对其他病毒检测结果为阴性.在荧光显色中分别应用SYBR Green Ⅰ和钙黄绿素作为显色剂,其结果与琼脂糖凝胶电泳一致.在对80份临床样本的检测中,使用LAMP和PCR检测出阳性样本的数量分别为68份和55份.因此,基于荧光显色的LAMP方法可以应用于ARV的快速检测,具有在科研机构、基层实验室特别是检测现场推广和应用的潜力.     

铁苋菜提取物对仔猪生长性能、血液生化指标及肠道菌群的影响

目的:本试验旨在研究铁苋菜提取物对仔猪生长性能、血液生化指标及粪样菌群的影响.方法:选取同一批次的25日龄健康的外三元断乳仔猪120头,平均体重为9.60+0.65 kg/头,随机分为4组,每组3个重复,每个重复10头,公母各半;试验组分别为铁苋菜提取物组Ⅰ和组Ⅱ、抗生素组及对照组,铁苋菜提取物组Ⅰ和组Ⅱ在基础日粮中分...     

Evaluation of immunogenicity and protective efficacy of Actinobacillus pleuropneumoniae HB04C(-) mutant lacking a drug resistance marker in the pigs

Previously, we reported the construction and characterization of a genetically defined Actinobacillus pleuropneumoniae (A. pleuropneumoniae) apxIIC gene mutant, HB04C(-), which conferred protection to mice against infection with A. pleuropneumoniae. In this study, we further evaluated HB04C(-) for safety and its ability to elicit protective immunity in pigs. It was demonstrated that a dose of 2 x 10(8) CFU HB04C(-) was safe to the pigs via intranasal or intramuscular injection. Immunization with a dose of 2 x 10(8) HB04C(-) by both intranasal and intramuscular routine could yield equal protective efficacy and elicited significant protection against experiment challenge with homologous or heterologous serotypes of a virulent A. pleuropneumonia. Taken together, HB04C(-) might serve as a promising vaccine candidate against infection with A. pleuropneumoniae.