Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.     

A mutation confers Monochoria vaginalis resistance to sulfonylureas that target acetolactate synthase

Acetolactate synthase (ALS) is the target enzyme for four distinct families of compounds: sulfonylureas (SUs), imidazolinones, triazolopyrimidine sulfonanilides, and pyrimidinyl oxybenzoates. We cloned and sequenced the fragments encoding ALS genes from biotypes of Monochoria vaginalis susceptible (S) and resistant (R) to SU-herbicides. The nucleotide sequences of the 39 bp Domain A region for R M. vaginalis biotype differed from that of the S biotype by a single nucleotide substitution at variable Pro codon of Domain A (CCT to TCT), predicting a Pro in the S but a Ser in the R biotype. No nucleotide differences between S and R M. vaginalis were observed in Domain D. We suggest that the amino acid substitution at Domain A region is responsible for resistance to SU-herbicides in M. vaginalis collected from Ushiku City, Ibaraki Prefecture, Japan.     

小叶锦鸡儿根瘤固氮活性的动态变化

对多年生小叶锦鸡儿不同生育期根瘤固氮能力、不同树龄小叶锦鸡儿根瘤固氮能力进行调查,结果表明:开花期固氮酶活性最高,此时土壤含氮量和植株含氮量最高;新瘤多发生于果后营养期,植株生物量的峰值出现在结实期;二年生和五年生植株的根瘤固氮能力强于三年、四年生植株,随生长年限的延长,植株生物量逐渐增大,含氮量呈小幅度下降趋势。     

Effect of gut stress induced by oxidized wheat gluten on the growth performance,gut morphology and oxidative states of broilers

This study was to investigate the effect of oxidized wheat gluten (OG) on growth performance, gut morphology and its oxidative states of broilers. One hundred and eighty‐day‐old male broilers (10 chicks/pen) were randomly allocated into three dietary treatments: control diet (CON), diet with 8% wheat gluten (WG) and diet with 8% OG with six pens/treatment. Body weight (BW) (21 and 35 days) and average daily gain (ADG) (1–21 days and 22–35 days) decreased (p < .05) and feed conversion ratio (FCR) (1–21 days and 22–35 days) increased (p < .05) in OG treatment. Feed intake (FI) decreased (p < .05) in WG and OG treatments during 22–35 days. However, FI was not influenced by dietary treatments during 1–21 days (p > .05). The OG‐fed broilers had a lower faecal pH value (p < .05) and higher faecal moisture content (p < 05) at 14, 21, 28 and 35 days. Villus height, crypt depth and V/C value were not different (p > .05) among treatments at 21 and 35 days. Lipid peroxidation (LPO) (21 and 35 days) and malondialdehyde (MDA) (35 days) content in crop of OG treatment increased (p < .05). Oxidized glutathione (GSSG) (21 days), LPO (21 and 35 days) and MDA (21 and 35 days) content in ileum of OG treatment increased (p < .05). The reduced glutathione/oxidized glutathione (GSH/GSSG) (21 days) and (GSH) (35 days) in ileum of OG treatment decreased (p < .05). The present findings indicate that OG might be a stressor for broiler gut, which could induce oxidative stress both in crop and in ileum, and the diarrhoea as well. The growth performance of broiler was consequently depressed.     

基于"互联网+"的草业企业经营管理模式——以草都易牧连锁超市为例

在"互联网+"大潮改造着各行各业的背景下,新时期的草业企业经营管理模式正发生着深刻的变化。基于此,以草业电商的实例——草都易牧连锁超市为例,分析其经营管理模式,并运用成本效益分析法从理论上探讨其经济可行性。结果表明,草都易牧连锁超市的经营管理模式为"互联网+草业"模式。该模式能将牧区饲草料的需求和供给对接起来,增加参与其中的线上牧户与入驻企业的利润,实现二者的共赢,在理论上具有经济可行性。草都易牧连锁超市的大胆尝试是草业企业在当前经济社会环境下一种重要的"互联网+草业"创新模式。通过对"互联网+"在草业中具体应用的深入探讨,认为草业企业的"互联网+草业"模式多主体分析、实证分析以及草产业链尺度分析或将成为"互联网+草业"未来的3个重点研究方向。     

不同盐胁迫水平下硅对高羊茅幼苗生物量、酶活性和渗透调节物质的影响

采用盆栽试验研究了高羊茅(Festuca arundinacea)幼苗在不同盐浓度胁迫下生物量、抗氧化酶活性和渗透调节物质对硅的响应。结果显示,随盐浓度增加高羊茅生物量逐渐降低,而盐浓度小于50 mmol·L~(-1)或大于250mmol·L~(-1)时,硅对高羊茅生物量没有明显影响,盐浓度介于50到250mmol·L~(-1),硅显著提高了高羊茅生物量(P0.05)。随着盐浓度增加过氧化氢酶(CAT)活性呈上升趋势,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性先上升后下降,添加硅虽然提高了POD、SOD和CAT活性,但不同酶显著增加时盐浓度存在一定的差异。可溶性糖和可溶性蛋白含量随着盐浓度的增加呈先上升后下降的趋势,丙二醛含量呈上升趋势,盐浓度为200和250mmol·L~(-1)时,可溶性糖和可溶性蛋白含量达到峰值,添加硅均降低了高羊茅体内可溶性糖、可溶性蛋白和丙二醛含量。上述结果表明,硅对高羊茅适应盐胁迫能力的影响在盐浓度间存在分异,过低或过高盐浓度时,硅对高羊茅适应性没有显著影响,中盐浓度时,硅能通过增强高羊茅体内的抗氧化酶活性、改善渗透调节过程和降低膜质氧化来增强其对盐胁迫的适应性。     

草鱼呼肠孤病毒生长特性研究

以草鱼呼肠孤病毒(GCRV)873毒株为试验材料,建立了GCRV荧光定量PCR检测方法;用GCRV873株感染CIK细胞及草鱼,通过对病毒RNA复制水平、子代病毒含量以及细胞病变等情况进行监测,研究其在CIK细胞及草鱼体内的生长特性。在CIK细胞中,GCRV感染后12 h即可检测到子代病毒,36 h时,所有细胞被感染;72 h时,病毒滴度达到最高,为10~(6.75) TCID_(50)/mL。在草鱼的肝、脾、肾中,均能检测到GCRV RNA,其中肾脏中含量最高,其次是肝脏,脾脏中最少。本试验为研究GCRV致病机理及制备细胞灭活疫苗奠定了试验基础。     

6种人兽共患病病原核酸液相芯片检测体系的建立

本研究通过设计、合成特异性引物和探针并对其进行修饰,将探针和特定荧光编码微球共价交联后,利用Luminex检测仪读取荧光信号,从而建立了针对布鲁氏菌、沙门氏菌、弓形虫,狂犬病病毒、巴氏通体、弯曲杆菌等6种病原的液相芯片检测方法。数据显示,本研究建立的液相芯片检测体系具有良好的特异性,检测灵敏度达50拷贝数,能同时检测6种重要人兽共患病病原,对于这6种人兽共患病的快速筛查和控制提供了技术支撑。     

百宜黑羽鸡外貌特征及生长发育研究

从选育中的1,2世代百宜黑羽鸡的外貌特征、生长性能两个方面对百宜黑羽鸡的部分特征特性进行了研究,结果表明:百宜黑羽鸡具有全黑羽带墨绿色(脚、胫、喙、耳毛为黑色),体形中等,肤白色。2周龄时公母均出现日增重率先升后降,分别由0.048%和0.48%上升到41.72%和37.79%,接着又下降到5.69%和5.06%。百宜母鸡的生长曲线从10周龄开始与公鸡的生长曲线分离,17周龄后母鸡的生长速度明显慢于公鸡。     

提高仔兔成活率的技术管理措施

从出生到断奶这段时间的兔称为仔兔,根据其生理特点,此阶段可分为睡眠期和开眼期,这是仔兔生长发育过程中非常重要而又很难饲养的时期.在养兔生产中,仔兔成活率的高低对经济效益有很大的影响,直接关系到养兔的成败.因此,加强仔兔饲养管理,采取有效的保护措施,提高仔兔成活率是养兔成功的关键.