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51.
Rounds L Havens CM Feinstein Y Friedman M Ravishankar S 《Journal of agricultural and food chemistry》2012,60(14):3792-3799
Meats need to be heated to inactivate foodborne pathogens such as Escherichia coli O157:H7. High-temperature treatment used to prepare well-done meats increases the formation of carcinogenic heterocyclic amines (HCAs). We evaluated the ability of plant extracts, spices, and essential oils to simultaneously inactivate E. coli O157:H7 and suppress HCA formation in heated hamburger patties. Ground beef with added antimicrobials was inoculated with E. coli O157:H7 (10(7) CFU/g). Patties were cooked to reach 45 °C at the geometric center, flipped, and cooked for 5 min. Samples were then taken for microbiological and mass spectrometry analysis of HCAs. Some compounds were inhibitory only against E. coli or HCA formation, while some others inhibited both. Addition of 5% olive or apple skin extracts reduced E. coli O157:H7 populations to below the detection limit and by 1.6 log CFU/g, respectively. Similarly, 1% lemongrass oil reduced E. coli O157:H7 to below detection limits, while clove bud oil reduced the pathogen by 1.6 log CFU/g. The major heterocyclic amines 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) were concurrently reduced with the addition of olive extract by 79.5% and 84.3% and with apple extract by 76.1% and 82.1%, respectively. Similar results were observed with clove bud oil: MeIQx and PhIP were reduced by 35% and 52.1%, respectively. Addition of onion powder decreased formation of PhIP by 94.3%. These results suggest that edible natural plant compounds have the potential to prevent foodborne infections as well as carcinogenesis in humans consuming heat-processed meat products. 相似文献
52.
Saltos Luis Alberto Corozo-Quiñones Liliana Pacheco-Coello Ricardo Santos-Ordóñez Efrén Monteros-Altamirano Álvaro Garcés-Fiallos Felipe R. 《Phytoparasitica》2021,49(1):113-122
Phytoparasitica - Root and crown rot (RCR) caused by Phytophthora capsici is present in all crop production areas of pepper and chili worldwide. This pathogen was recently reported at the Pacific... 相似文献
53.
Adelaide Almeida ?ngela Cunha Newton C.M. Gomes Eliana Alves Liliana Costa Maria A.F. Faustino 《Marine drugs》2009,7(3):268-313
Owing to the increasing importance of aquaculture to compensate for the progressive worldwide reduction of natural fish and to the fact that several fish farming plants often suffer from heavy financial losses due to the development of infections caused by microbial pathogens, including multidrug resistant bacteria, more environmentally-friendly strategies to control fish infections are urgently needed to make the aquaculture industry more sustainable. The aim of this review is to briefly present the typical fish farming diseases and their threats and discuss the present state of chemotherapy to inactivate microorganisms in fish farming plants as well as to examine the new environmentally friendly approaches to control fish infection namely phage therapy and photodynamic antimicrobial therapy. 相似文献
54.
Evaluation of DRAINMOD using saturated hydraulic conductivity estimated by a pedotransfer function model 总被引:1,自引:0,他引:1
Direct measurement of soil saturated hydraulic conductivity (Ks) is time-consuming and therefore costly. The ROSETTA pedotransfer function model is able to estimate Ks from soil textural data, bulk density and one or two water retention points. This study evaluated the feasibility of running the DRAINMOD field-scale hydrological model with Ks input produced using ROSETTA. A hierarchical approach was adopted to estimate Ks using ROSETTA, with four limited-more extended sets of soil information used as inputs: USDA textural class (H1); texture (H2); texture and bulk density (H3); texture, bulk density, water retention at −33 kPa (θ33 kPa) and −1500 kPa (θ1500 kPa) (H4). ROSETTA-estimated Ks values from these four groups (H1-H4) were used in DRAINMOD to simulate drain outflows during a 4-year period from a conventional drainage plot (CD) and two controlled drainage plots (CWT1 and CWT2) located in south-east Sweden. The DRAINMOD results using ROSETTA-estimated Ks values were compared with observed values and with model results using laboratory-measured Ks values (H0). Deviations in simulated drainage outflow (D), infiltration (F) and evapotranspiration (ET) resulting from the use of ROSETTA-estimated rather than laboratory-measured Ks values were evaluated. During the study period, statistical comparisons showed good agreement on a monthly basis between observed and DRAINMOD-simulated drainage rates using five soil datasets (H0, H1, H2, H3 and H4). The monthly mean absolute error (MAE) ranged from 0.57 to 0.82 cm for CD, 0.38 to 0.41 cm for CWT1, and 0.15 to 0.22 cm for CWT2. On a monthly basis, the modified coefficient efficiency (E′) values were in the range of 0.62 to 0.74 for CD, 0.72 to 0.74 for CWT1, and 0.79 to 0.86 for CWT2. The modified index of agreement (d′) for monthly predictions ranged from 0.80 to 0.86 cm for CD, 0.87 to 0.88 cm for CWT1, and 0.89 to 0.93 cm for CWT2. The absolute values of the percent-normalised error (NE) on an overall basis when using ROSETTA-estimated rather than laboratory-measured Ks values were less than 3% in E, less than 1% in F, and less than 15% in D. The results suggest that ROSETTA-estimated Ks values can be used in DRAINMOD to simulate drainage outflows as accurately as laboratory-measured Ks values (H0) in coarse-textured soils. 相似文献
55.
Cárdenas Liliana Cañas-Álvarez Jhon J. Vázquez Ana Boixadera Ester Casal Jordi 《Tropical animal health and production》2019,51(2):383-388
Tropical Animal Health and Production - Bovine brucellosis is a zoonotic disease that causes great economic losses. The disease is endemic in Colombia and animal movements from infected herds carry... 相似文献
56.
Liliana Mutascio Federico Vilaplana Grosso Jos Ramos‐Vara Micha Simons 《Veterinary radiology & ultrasound》2019,60(2):E10-E14
A 10‐month‐old German Shepherd Dog presented for evaluation of intermittent vomiting. Abdominal radiographs revealed a marked right cranial mass effect. Initial differentials included abscess/cyst or less likely neoplasia from undetermined origin. On abdominal ultrasound the mass appeared cystic and thin walled. Computed tomography revealed a large cystic lesion originating from the pyloroduodenal junction causing pyloric outflow obstruction. A noncommunicating duodenal duplication cyst was found on exploratory laparotomy and further confirmed with histopathology and immunohistochemistry. Enteric duplication cyst should be considered as a differential in young dogs with gastrointestinal signs and a cystic abdominal mass detected with different imaging modalities. 相似文献
57.
Luis Felipe Falla Zuñiga Yhoiss Smiht Muñoz Cerón Liliana Salazar 《Anatomia, histologia, embryologia》2021,50(1):206-211
Alterations in heart valve development represent more than 20% of congenital cardiovascular malformations. Most of the functional properties of heart valves depend on extracellular matrix. Despite its relevance, little is known about fibrillar components on developing stages. Our objective is to define histological changes on valves fibrillar components in late embryonic development of Mus musculus. We found type III collagen as the predominant fibre type in the ECM in prenatal stages followed by a switch to a type I predominance for postnatal ages. The change in fibrillar components is necessary to support the normal mechanical function of adult heart valves. 相似文献
58.
Though generally small and gene rich, bacterial genomes are constantly subjected to both mutational and population-level processes that operate to increase amounts of functionless DNA. As a result, the coding potential of bacterial genomes can be substantially lower than originally predicted. Whereas only a single pseudogene was included in the original annotation of the bacterium Escherichia coli, we estimate that this genome harbors hundreds of inactivated and otherwise functionless genes. Such regions will never yield a detectable phenotype, but their identification is vital to efforts to elucidate the biological role of all the proteins within the cell. 相似文献
59.
Properties of an aged phenanthrene-contaminated soil and its response to bioremediation processes 总被引:1,自引:0,他引:1
Purpose
Persistent organic compounds exhibit declining extractability and bioavailability to microorganisms and other soil organisms with increasing contact time or ageing. Among the possible mechanisms are the association of organic compounds with natural organic matter. Ageing can reduce the negative effects that an organic pollutant may have on the biological, biochemical properties and phytotoxicity of the contaminated soil. The aim of the present work was to evaluate, under laboratory conditions, the response of an agricultural soil contaminated with phenanthrene (Phe) and subjected to 2-year ageing and the effects and potential remediation capabilities of compost and an effective Phe-degrading bacterial culture. 相似文献60.
Liliana de Oliveira Rocha Gabriela Martins Reis Raquel Braghini Estela Kobashigawa Jansen de Araújo Benedito Corrêa 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(3):353-366
Aflatoxins can cause great economic losses and serious risks to humans and animals health. The largest aflatoxin producers
belong to Aspergillus section Flavi and can occur naturally in food commodities. Studies showed that molecular tools as well as the type of sclerotia produced
by the strains could be helpful for identification of Aspergillus species and could be correlated with levels of toxin production. The purpose of this work was to characterize the genetic
diversity using AFLP technique, the type of sclerotia and the ability of aflatoxin production by isolated strains from corn
of different origins in Brazil, and to verify whether qPCR based on aflR and aflP genes is appropriate for estimating the level of aflatoxin production. All the 75 strains were classified as A. flavus and the AFLP technique showed a wide intraspecific variability within them. Regarding sclerotia production, 34% were classified
as S and 66% as L type. Among the aflatoxin-producers, 52.8% produced aflatoxin B1, while 47.2% aflatoxins B1 and B2. Statistical analysis showed no correlation between sclerotia production and aflatoxigenicty, and no correlation between
the phylogenetic clusters and aflatoxin production. Concerning the relative expression of aflR and aflP, Pearson’s correlation test demonstrated low positive correlation between the expression of the aflR and aflP genes and the production of AFB1 and AFB2, but showed high positive correlation between aflR and aflP expression. In contrast to the other reference strains, A. oryzae ATCC 7282 showed no amplification of aflR and aflP. The results highlight the need for detection of reliable and reproducible markers with a high positive correlation with aflatoxin
production. 相似文献