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991.
Ferreira-Dias GM Serrão PM Durão JF Silva JR 《American journal of veterinary research》2001,62(4):526-530
OBJECTIVE: To document uterine growth and microvascular development in the endometrium of uteri with differing degrees of fibrosis as well as uterine growth throughout the estrous cycle of mares. ANIMALS: 30 mares. PROCEDURE: Uterine tissue was obtained during the breeding season from a slaughter facility. Stage of estrous cycle of the mares was assessed on the basis of ovarian structures and plasma progesterone concentrations. Endometrium was characterized by use of light microscopy, and blood vessel walls were marked by histochemical techniques. Microvascular development was evaluated by a computerized image analysis system. Growth of uterine tissue was based on cellular content of DNA and RNA, RNA:DNA, and protein:DNA. RESULTS: Significant differences in vascular density were not observed in the endometrium of uteri obtained from mares euthanatized during the follicular or luteal phase of the estrous cycle, regardless of whether endometrial classification of degree of fibrosis was considered. There was a 3-fold increase in amount of DNA and RNA of endometrial cells in the follicular phase when compared to myometrium. Hypertrophy of endometrial tissue during the luteal phase was reflected by a significant increase in cell protein content and protein:DNA. CONCLUSIONS AND CLINICAL RELEVANCE: Endometrial growth of vascular tissues during the estrous cycle may be coordinated with development of nonvascular tissue. Estrogen and progesterone may play a role in regulation of uterine growth and angiogenesis. 相似文献
992.
Szatmári V Osi Z Manczur F 《Journal of the American Veterinary Medical Association》2001,218(11):1796-9, 1778-9
Pyonephrosis refers to suppurative destruction of the parenchyma of the kidney with complete or nearly complete loss of renal function. In dogs, nephrectomy is still the most common treatment for pyonephrosis; however, in the present report, a method for percutaneous ultrasound-guided drainage of the renal pelvis in dogs with pyonephrosis that does not require local or general anesthesia was described, and results of the procedure in 2 dogs were reported. Briefly, dogs were positioned in lateral recumbency with the affected side up, and skin overlying the affected kidney was aseptically prepared. The dilated renal pelvis was punctured percutaneously, under ultrasound guidance, with a 22-gauge needle, and a sample of material was obtained for analysis. The needle was then replaced with an IV catheter, and as much pus as possible was removed from the renal collecting system. A povidone iodine solution was then used to lavage the renal pelvis. Ultrasound-guided drainage and lavage of the renal pelvis was repeated daily until the renal pelvis was so small that it could no longer be punctured. Both dogs recovered and were reported by the owners to be healthy after the procedure. 相似文献
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997.
Nutrient intake during gestation has an impact on gestation parameters and subsequent lactation performance. The objectives of this experiment were to determine the impact of feeding two levels of amino acids in gestation on sow BW changes in gestation and lactation, and litter size, and to evaluate a factorial method for determining daily energy requirements. At mating, 419 sows (Camborough 15; Pig Improvement Canada, Acme, AB) were assigned randomly within Parities 1, 2 or 3+ to a gestation diet containing either 0.44% (low lysine) or 0.55% (high lysine) total lysine and 3,100 kcal DE/kg; other indispensable amino acids were adjusted to lysine based on ideal protein ratios. Feed allowance in gestation was determined factorially using estimated DE requirements for maintenance, maternal gain, and conceptus growth. Sows were allowed free access to the lactation diet. Gestation BW gain from d 0 to 110 was affected by parity (61.2, 60.0, and 42.3 kg for Parity 1, 2, and 3+, respectively; P < 0.05) but not (P > 0.10) by gestation lysine level. Sow BW changes from d 0 of lactation to weaning were affected by parity (0.5, 6.8, and 5.8 kg for Parity 1, 2, and 3+, respectively; P < 0.01) and gestation BW gain (P < 0.01), but not by gestation lysine level (5.0 vs 3.8 kg for low and high lysine, respectively; P > 0.10). Total piglets born was affected by parity (11.5, 12.1, and 12.5, for Parity 1, 2, and 3+, respectively; P < 0.01) and increased with increasing sow BW gain (P < 0.05). Total piglets born alive (mean = 11.2) was increased with increasing sow BW gain (P < 0.05). Total litter weight born alive was affected by parity (15.9, 18.6, and 19.4 kg for Parities 1, 2, and 3+, respectively; P < 0.01) and gestation BW gain (P < 0.05). The model used to determine daily energy intake requirements resulted in an average BW gain of 10.6 kg above the targets set by the model. Total lysine intakes greater than 10.6 g/d in gestation did not improve sow productivity. Setting target weight gains in gestation and feeding to meet these targets may not always provide predictable results due to a number of factors that affect the energy requirement in the sow. 相似文献
998.
Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro 总被引:4,自引:0,他引:4
Wallace RJ Wallace SJ McKain N Nsereko VL Hartnell GF 《Journal of animal science》2001,79(7):1905-1916
This study was done to determine the effectiveness of supplementary enzymes at increasing the fiber digestion by ruminal microorganisms and to assess whether enzyme activity limits the rate of fiber digestion in ruminal digesta. In vitro comparisons of enzyme activities in two feed enzyme preparations (A and B) with enzyme activities extracted from ruminal fluid indicated that the addition of fibrolytic enzymes at the application rates recommended by the manufacturers would not be expected to increase significantly glycanase and polysaccharidase activities in ruminal fluid. Preparations A and B both increased (P < 0.001) the rate of gas production from freeze-dried corn and grass silages in in vitro incubations with ruminal fluid, but only at concentrations much higher than recommended application rates. Autoclaved controls had little or no effect. Ultrafiltration of enzyme B indicated that most stimulation was due to components >100 kDa, which is consistent with the cause of the stimulation being enzyme activity. Fibrolytic enzymes from other sources were also able to stimulate gas production: increased rates of gas production were observed in seven out of eight combinations of "cellulase" and corn or grass silage (P < 0.05). The comparison of glycanase and polysaccharidase activities with gas-stimulatory activity in the different enzyme preparations indicated that the highest correlation was between increased gas production and enzyme activity against microgranular cellulose (P < 0.05). In a wider range of fibrolytic enzyme preparations, those with endo-(beta-1,4)- or exo-(beta-1,4)-xylanase activity equal to that of preparation A did not produce similar increased rates of fermentation of corn silage when glucanase activity was low (P > 0.05). In contrast, preparations with glucanase activity similar to enzyme A gave at least as great (P < 0.05) an improvement in gas production than enzyme A, irrespective of xylanase activity. It was concluded that enzyme activity, probably a type of endo-(beta-1,4)-glucanase activity, limits the rate of fermentation of corn and grass silage in the rumen. Enzyme supplements of the type used in these experiments are unlikely to possess sufficient activity to overcome this limitation by direct application to ruminal digesta, implying that treatment of the ration prefeeding will be key to harnessing the potential of exogenous fibrolytic enzymes in ruminant nutrition. 相似文献
999.
Stöcklin-Gautschi NM Guscetti F Reichler IM Geissbühler U Braun SA Arnold S 《The Journal of small animal practice》2001,42(8):413-416
A focal uterine adenomyosis is described in two bitches. In both cases, the uterus showed knobbly enlargements of 4 to 8 cm in diameter, which resulted in distinct clinical symptoms. Other pathological changes of the uterus were not present. One bitch was presented because of a history of vaginal discharge of several months' duration. Radiographs, as well as ultrasonography, revealed a soft tissue lesion at the cervix. The other bitch showed a marked reduction in its general condition and a sudden onset of a tense abdomen. Radiologically, a lesion of soft tissue opacity was observed in the mid-abdomen and was seen to originate from the left uterine horn during exploratory laparotomy. A torsion of the lesion was present, which explained the clinical signs in this second case. 相似文献
1000.
The prevalence of chronic wasting in cattle in March and April 2000 was studied on 218 dairy farms with a history of health problems accompanied by wasting, following reports in the media suggesting that chronic wasting was a substantial problem on Dutch dairy farms. A telephone call revealed that the health problems had resolved on 41 farms; 16 of these farms had culled all cattle. Two farmers refused co-operation. On the remaining 175 farms the animals were inspected and was completed a questionnaire. A high percentage of culling for of health reasons (on average 18.1% of young stock and adult cattle) and an increased mortality rate (4.8%) were reported on the farms visited. In only two of the 175 inspected herds, more than 20 percent of cattle were found showing signs of wasting. These two herds were identified as 'chronic wasting herds'. The prevalence of such herds was low in this study. Consequently, it is likely that there were very few 'chronic wasting herds' among the whole Dutch dairy population in March/April 2000. 相似文献