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91.
Adriano O. T. Carrasco Meire C. Seki Ricardo L. M. de Sousa Tânia F. Raso Aramis A. Pinto 《Tropical animal health and production》2009,41(7):1325-1333
The purposes of this study were to model a vaccination regimen for Newcastle disease virus (NDV) in pigeons, and to evaluate
the susceptibility and behavior of vaccinated birds against a highly pathogenic NDV Brazilian strain. Antibody response was
assessed by means of hemagglutination inhibition test (HI), and viral genome excretion by means of RT-PCR. Vaccinal strains
(La Sota and Ulster) induced high antibody titers without any adverse effects, both in inoculated and in sentinel birds. A
viral strain pathogenic for chickens did not produce clinical signs of the disease in experimentally infected pigeons. Only
4 out of 10 vaccinated pigeons shed NDV genome, and just for two days. Results confirmed the high infectivity of the vaccinal
strains used, as all nonvaccinated pigeons showed antibody titers as high as those of vaccinated birds. 相似文献
92.
Sánchez-Cordón PJ Romanini S Salguero FJ Ruiz-Villamor E Carrasco L Gómez-Villamandos JC 《Veterinary pathology》2003,40(3):254-262
The aim of this study was to report on the lesions occurring in the intestine during experimental classical swine fever (CSF) and to clarify the nature of infected cells and the distribution of viral antigen. Thirty-two pigs were inoculated with the virulent CSF virus (CSFV) isolate Alfort 187 and slaughtered from 2 to 15 postinoculation days; four animals of similar background served as a control group. Immunohistochemistry, electron microscopy, and the transferase-mediated deoxyuridine triphosphate nick-end labeling method were used to detect viral antigens and apoptosis. The results showed progressive lymphoid depletion and mucosal necrosis. The lymphoid depletion could have been caused by apoptosis of lymphocytes but could not be directly attributed to the effect of CSFV on these cells. Vascular changes, pathogenic bacteria, and viral infection of epithelial cells were ruled out as causes of necrotic lesions. However, large virally infected monocytes-macrophages with ultrastructural changes indicative of activation were observed in the intestine. This suggests that monocytes-macrophages play an important role in the pathogenesis of intestinal lesions. An understanding of the function of these cells will require additional study. 相似文献
93.
Oestrogen and progesterone receptors in feline fibroadenomatous change: an immunohistochemical study
Martín De Las Mulas J Millán Y Bautista MJ Pérez J Carrasco L 《Research in veterinary science》2000,68(1):15-21
The distribution of oestrogen and progesterone receptors was analysed in 18 cases of feline fibroadenomatous change (FFAC) using commercially available specific monoclonal antibodies and the Avidin-biotin peroxidase complex technique on formalin-fixed, paraffin-embedded tissue. In all cases of FFAC, progesterone receptors were detected either in epithelial cells (mostly suprabasal) or in epithelial and stromal cells. Oestrogen receptors were detected in approximately half of these cases, in suprabasal or luminal epithelial cells exclusively. Myoepithelial cells lacked both oestrogen and progesterone receptors. The techniques used have identified the specific cellular distribution of steroid hormones receptors in this hormone-dependent lesion of the feline mammary gland. The results confirm those of previous biochemical analyses with respect to progesterone receptors and add new data concerning the possible involvement of oestrogen receptors and stromal fibroblasts in the hormonal control and development of the lesion. 相似文献
94.
95.
Azorit C Hervas J Analla M Carrasco R Muñoz-Cobo J 《Anatomia, histologia, embryologia》2002,31(4):224-227
A method to obtain thin histological sections (4-5 microm thick) from teeth, using a conventional microtome, is described for incisors, molars and canines from Spanish red deer (Cervus elaphus hispanicus). Decalcifying solutions of formic acid and nitric acid at different concentrations were ineffective. This was not the case for a solution of 14% hypochloric acid and polyvinylpyrrolidone (TBD-1(R) of Shandon). However, the time needed to complete decalcification was longer than the time estimated by other authors. Incisors required between 4 and 6 days, molars between 7 and 15 days, and canines between 8 and 12 days. The preparation of histological thin-sections from teeth with a conventional microtome, required some training or prior specialization, because most of defective or lost material occurred during the preliminary stage of the study. However this technique is inexpensive enough to be implemented in any histological laboratory. The preparations obtained are suitable for growth mark observation in dentine and in cementum for dating studies. Nevertheless, they are not suitable for enamel change studies, because the enamel is completely destroyed during the decalcifying process. 相似文献
96.
Arenas AJ Gómez F Salas R Carrasco P Borge C Maldonado A O'Brien DJ Martínez-Moreno FJ 《Veterinary parasitology》2002,109(1-2):111-117
The application of infrared thermal imaging to the diagnosis of sarcoptic mange in the wild Spanish ibex (Capra pyrenaica) was evaluated. Seventy-three ibexes (35 males, 38 females) of varying ages were studied. Each animal was observed using conventional binoculars (OT) to detect lesions characteristic of mange. Infrared thermography (IR) was then performed and the resultant image judged negative or positive. The distance from the thermograph to the animal was measured, and the animal killed. Skin samples were taken for mite detection by routine laboratory diagnosis (LAB). The most sensitive and specific technique for the tele-diagnosis of sarcoptic mange in the Spanish ibex is OT, as it permits diagnosis over greater distances than IR, which sensitivity is impaired at distances >100m. When disease prevalence is low, such as in initial and final phases of an epidemic, a more sensitive technique would be valuable in detecting all affected animals. 相似文献
97.
Luque I Tarradas C Carrasco L Torroella E Artigas C Perea A 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(6):445-451
The effectiveness of medication with doxycycline in feed in the control of pleuropneumonia in pigs was tested using an Actinobacillus pleuropneumoniae serotype 1 aerosol challenge model. Two groups of 10 animals were used for the challenge, a 'medicated group' and an 'unmedicated group'. A third group of four animals was used as a 'control group'. Pigs from the medicated group were provided with feed containing 250 p.p.m. doxycycline (HIPRAMIX/DOXI) for 8 consecutive days and were challenged on the fifth day of treatment. No clinical signs were observed in pigs from the 'control group'. Four animals from the 'unmedicated group' died within the first 48 h after challenge with clinical and lesional evidence of an acute form of pleuropneumonia. Clinical signs of animals surviving the first 48 h were progressively less severe and showed lesions similar to those described for subacute-chronic forms of the disease. However, only one animal from the 'medicated group' showed clinical signs of a chronic form of pleuropneumonia. Reisolation of A. pleuropneumoniae was more evident from lung tissues of animals fed the doxycycline-free feed (70%), coinciding with the presence of both acute and subacute lesions. However, the micro-organism could be reisolated from only one animal which belonged to the 'medicated group'. It is concluded that the treatment of pigs with 250 p.p.m. doxycycline (HIPRAMIX/DOXI) prevents disease caused by A. pleuropneumoniae. 相似文献
98.
99.
Aliro Borquez Edison Serrano Patricio Dantagnan Jaime Carrasco Adrian Hernandez 《Aquaculture Research》2011,42(8):1067-1078
The effect of dietary inclusion of whole grain white lupin (Lupinus albus) on growth performance, histology, muscle fatty acid composition and nutrient digestibility was investigated in an 11‐week growth and a 4‐week digestibility trial with rainbow trout (initial body weight of 54.0 ± 6.2 and 181.9 ± 3.4 g respectively). Four experimental extruded diets were formulated to contain 0%, 30%, 40% and 50% of whole grain lupin and fed to triplicate groups of fish twice a day until apparent satiation. Faeces were collected daily from each digestibility tank by decantation. No significant trends were observed with respect to growth, feed utilization, apparent digestibility coefficients or whole‐body composition (P>0.05). Conversely, increasing levels of dietary lupin led to significant decreases in the Hepatosomatic index (R2=0.75, P<0.05) and slight lipid infiltration into hepatocytes and enterocytes. Muscle fatty acid compositions were slightly affected by the dietary treatment. Polynomial regression of dietary inclusion of lupin and muscle fatty acid concentrations showed an increase in C18:1n‐9, C18:2n‐6 and C18:3n‐3 and a decrease in C20:5n‐3 with increasing dietary lupin level. These results demonstrated that whole grain lupin can be included up to 50% in commercial rainbow trout diets without negative effects. 相似文献
100.
Ramón?CarreresEmail author Juan?Sendra Rafael?Ballesteros Eduardo?Fernández?Valiente Antonio?Quesada David?Carrasco Francisco?Leganés José?García?de la?Cuadra 《Biology and Fertility of Soils》2003,39(2):80-87
This work evaluates the effect of different slow-release fertilizers and nitrification inhibitors (NI) on N-use efficiency, grain yield and N2 fixation in rice fields of Valencia (Spain) during three consecutive crop seasons (1998–2000). Eight N sources [ammonium sulphate, urea, polymer-coated urea (PCU 32% and 40% N), sulphur coated urea, isobutylidene diurea (IBDU), ammonium sulphate nitrate (ASN) plus dicyandiamide and ASN plus dimethyl pyrazole phosphate, were applied at 120 kg N ha–1 and at 2 or 15 days before flooding (DBF) during 1998. Another experiment was based on the use of urea blended with PCU (40% N) at four ratios (1:0, 3:1, 1:1, 1:3) and applied at 15 DBF and at four rates (70, 95, 120 and 145 kg N ha-1) during 1999 and at only one rate (120 kg N ha–1) during 2000. Both experiments also included a control (no N). The results showed that, when applied shortly before flooding, PCU (32% and 40% N) and IBDU application improved biological N2 fixation compared to the conventional fertilizer application, with or without NI, reaching similar values to those observed in the absence of N fertilizer. Slow release fertilizers, particularly PCU 40% N applied basally before flooding, were the best N source for grain yield and improved recovery efficiency. Differences among N sources were only significant when the flooding was delayed for 15 days after fertilizer application. 相似文献