二十碳四烯酸研究

花生四烯酸（Arachidonic acid,AA）是n-6多不饱和脂肪酸（PUFAs）。AA的系统命名是全顺-5,8,11,14-二十碳四烯酸,又名5,8,11,14-花生酸,简记为20：4n-6,分子量304．5,分子式C20H32O2,结构式如图1所示.     

桃(Prunus persica L.)茎尖培养技术的研究

筛选出了适合于桃品种‘北农早艳’茎尖(0.2 mm 和0.5 mm)培养的培养基为:G培养基附加 IBA 0.25 mg/L+BA 0.5 mg/L+GA_3 1.0 mg/L+LH 500.0 mg/L。茎尖用聚氨酯海绵作支撑物的液体培养比琼脂培养成活率高2～5倍,且生长迅速。获得了0.2 mm 长茎尖的生根植株。     

追踪实战基本技能的训练

警犬追踪实战情况错综复杂,既有客观条件的变化,如案件性质、使用条件、现场环境和地理气候等;又有主观因素的影响,如人的分析判断能力、犬的作业状况等。面对各种各样复杂的现场,带犬民警作为警犬使用的主体,必须具备对案件现场勘察、分析、判断的基本功,具有正确指挥和使用警     

Molecular cloning and expression of kidney‐type glutaminase from common carp (Cyprinus carpio) and its up‐regulation by glutamine in primary culture enterocyte

Glutaminase (GLS) is the key enzyme of glutamine (Gln) metabolism and utilization. In this study, a cDNA encoding GLS protein was identified from common carp Cyprinus carpio intestine. The open reading frame of GLS cDNA encodes a polypeptide of 595 amino acids, which shows a high similarity with its zebrafish Danio rerio counterpart. Bioinformatic analysis showed the protein belongs to kidney‐type GLS. The putative protein has glutaminase domain and ankyrin repeats domain, which are highly conserved among vertebrate orthologues. Real‐time quantitative PCR analysis revealed that the abundance of GLS mRNA was the highest in the white muscle, followed by the brain, eyeball and pituitary. Glutaminase was ubiquitously expressed in all intestinal segments of common carp. The activity of GLS did not distribute uniformly along the entire length of the intestine. In primary culture enterocyte, and the expression of GLS mRNA is up‐regulated quickly and effectively by Gln.     

聚L-乳酸改性麦草共混物材料的研究

以聚L-乳酸(PLLA)和麦草(WS)为原料,以N-甲基吗啉-N-氧化物(NMMO)为溶剂,采用溶液共混法制备了PLLA/WS共混物。首先将麦草溶解在NMMO后,再添加聚L-乳酸并溶解,制备聚L-乳酸与麦草的共混溶液。共混溶液采用浇膜法制备PLLA/WS共混物,采用差示扫描量热法、X射线衍射法、傅里叶变换红外光谱、热重分析和扫描电镜等方法对所得的共混物进行表征。结果表明,聚L-乳酸与麦草可以形成均匀的共混溶液。共混物中PLLA与麦草组分之间具有较强的相互作用,相容性较好。当共混物中PLLA的质量分数为50%时,共混物可以形成结晶结构,且随着PLLA含量的增加,其结晶更加完美,熔点与热稳定性提高。共混物薄膜断面结构较为致密,这说明PLLA与麦草相容性较好,混合均匀。通过调节聚L-乳酸和麦草的配比,可以制备不同性能的生物高分子材料。     

开阳县田坝渔业小区实施大鲵养殖的技术措施

为摸索名优水产品养殖经验,加快我县水产养殖结构调整,促进我县渔业健康发展,2007年,我县申报实施了《贵阳市社会主义新农村基础设施建设——养殖基地基础设施建设——渔业小区建设》项目,在花梨乡翁昭村田坝组模拟大鲵生态生活习性新建了100口养殖池的大鲵小区,修建了养殖房、管理房、配套饵料鱼池、饵料鱼暂养池、引水管道等基础设施和配套设施,并于2008年3月25日起引种入场养殖。现将1年来小区开展大鲵人工养殖的技术措施报告如下。     

现浇楼板混凝土“二次法”施工工法

阐述了现浇楼板混凝土施工时,混凝土终凝前通过二次振捣的施工方法,愈合混凝土内部和表面初裂缝,以提高混凝土密实度,达到控制混凝土裂缝的目的。     

非洲菊切花保鲜剂的研究

本文研究由蔗糖、硝酸银、8-HQ(8-羟基喹啉)配制的不同保鲜剂对非洲菊切花插瓶寿命、水分平衡、鲜重变化及观赏值等指标的影响。结果表明：4％蔗糖 200mg／L硝酸银 200mg／L硫酸铝 320mg／L8-HQ的保鲜效果最好。     

链格孢菌（Alternaria tenuissima）cDNA酵母表达文库的构建

为了解链格孢菌（Alternaria tenuissima）遗传学背景,克隆及研究该菌功能基因,根据Gateway技术构建了既能在酵母细胞中表达外源基因又能在原核细胞大量复制的酵母表达载体pRS-DEST42,构建了细极链格孢菌（A. tenuissima）cDNA酵母表达文库。经检测,该文库的平均滴度为2.44×106（cfu/ml）,文库总容量为2.44×107,阳性克性率为100%,平均插入片段约为1.38kb。细极链格孢菌（A. tenuissima）cDNA酵母表达文库是一个质量较高的表达文库,为克隆与分离全长目的基因及其功能奠定了坚实的基础。     

Pharmacokinetics of florfenicol in healthy and Escherichia coli-infected broiler chickens

The aims of this study were to evaluate the effects of a ketamine/propofol anaesthetic protocol in lions (Panthera leo), and to compare it to two commonly used anaesthetic protocols. Seventeen adult lions were anaesthetised using three different protocols. Group XK (n=6) was anaesthetised with intramuscular (i.m.) injections of xylazine and ketamine. Group KD (n=5) was anaesthetised with an i.m. injection of ketamine, followed by an intravenous (i.v.) injection of ketamine and diazepam. Group KP (n=6) was anaesthetised with an i.m. injection of ketamine followed by an i.v. injection of propofol. There was a significant difference in heart rate (P<0.0002), which was lowest in group XK and highest in KD. Jaw tone was significantly lower in Group XK (P<0.05). No undesirable effects were noted following injection of the propofol. Propofol was a suitable and safe drug for maintenance of anaesthesia in adult lions.