Application of a fully automatic analysis tool to assess the activity of broiler chickens with different gait scores

Monitoring the activity of broilers is a potential way for determining gait score level at commercial farms. In this study, a fully automatic monitoring technique was developed to measure the activity of broiler chickens with different gait score levels. We carried out two experiments in order to assess the relationship between gait scores obtained by human experts and activity levels quantified by an automatic image monitoring system. The chickens were scored for their degree of lameness by experts according to the method of Kestin et al. (1992). For each experiment, a total of 30 birds, on day 32 were selected from a local commercial farm. Five birds were selected in each of six gait score groups (GS0-GS5). The activity levels were obtained for all gait scores by using an automatic image monitoring system. For this purpose, video surveillance images of broilers with six different predefined gait scores were analysed. In both experiments, there was a significant relation between gait score by experts and activity monitored by image analysis. The broilers with gait score 3 (GS3) showed significantly higher activities than the other gait scores in both experiments (P < 0.05), possibly due to their need for more feed. In both experiment, GS4 and GS5 showed significantly lower activities (P < 0.05). Overall, the results show that automatic camera monitoring system can provide an automatic tool in determination the activity in relation to gait score. This activity information can be used further to identify the effects of gait score on broiler behaviour.     

Efficacy of mating disruption technique against carob moth,Apomyelois ceratoniae Zeller (Lepidoptera: Pyralidae) in pomegranate orchards in Southeast Turkey (Şanlıurfa)

Carob moth, Apomyelois (=Ectomyelois) ceratoniae Zeller (Lepidoptera: Pyralidae) is a key pest in pomegranate orchards in Southeast of Turkey. The pest causes significant damage and reduces marketability of fruits, and is difficult to control with insecticides. Therefore, it is imperative to develop environmentally sound effective alternative management approaches. In this study, we aimed to evaluate the mating disruption technique and determine the appropriate application dose for the management of the pest. The study was carried out in Central and Suruç districts of ?anl?urfa province (Turkey) in 2011 and 2012. SPLAT EC (Specialized Pheromone-Lure Application Technology) trade named dispenser containing 2% Z7, E9-11-dodecatrienyl formate pheromone mimic was applied at 500, 625 and 750 g/ha in three pomegranate orchards in each district. Results indicated that mating disruption technique significantly reduced the pest populations. But effectiveness of the method was less in terms of the damage rate on fruits. Pheromone applied at 500 g/ha was not effective. However, application at 625 g/ha reduced the pest populations to an acceptable level. Thus, the concentration could be considered an appropriate dose for the less abundant pest population within integrated pest management while higher population would require combining the mating disruption method with a bio-insecticide.     

Control of the pink bollworm <Emphasis Type="Italic">Pectinophora gossypiella</Emphasis> by the mating disruption technique on cotton in a semi-arid region of Turkey

This study evaluated the effectiveness of mating disruption pheromones for the pink bollworm (PBW) Pectinophora gossypiella (Saund.) (Lep.: Gelechiidae) and determined the damage levels of cotton plants in Turkey in 2007 and 2008. Adult populations were monitored by pheromone traps, with 250 pieces ha⁻¹ of PBW rope dispensers used for each treated field of approximately 12 ha. Calculations were based on comparing cotton yield in the control field with that in treated fields. The PB-Rope L dispensers significantly decreased both number of males in the pheromone traps and cotton boll damage. Results showed that PB-Rope L dispenser application during the square period of cotton plants effectively reduced damage. Cotton yield of treated fields was increased compared with control fields by 20.28% and 10.28% in 2007 and 2008, respectively. Infestation ratios were determined by comparing cotton bolls collected from treated fields with bolls from control fields; the results were 25% and 15% for control fields and 16% and 4% for treated fields in 2007 and 2008, respectively. Larval densities in the control fields were higher than those in the treated fields in both years. Results showed that the PB-Rope L dispensers released pheromone satisfactorily to disrupt pest mating.     

The Effects of Sex and Seasonality on the Metal Levels of Warty Crab (Eriphia verrucosa) in the Black Sea

The present paper examines the impacts of gender and season variables on the nutritional composition and metal levels of warty crab caught in the central Black Sea region (Ordu). Crab samples were caught over four seasons, using gillnets, trammel net, and SCUBA diving. Results of chemical analysis showed that protein, lipid, moisture, and total mineral substance level ranges of male crabs were 20.45–22.64%, 0.95–1.27%, 75.44–77.09%, and 1.63–2.08%, while these ranges in female individuals were determined as 19.45–21.46%, 0.63–0.92%, 75.44–77.44%, and 2.08–3.28%, respectively. Macro and trace element levels in crab muscle tissue were determined as K > P > Na > Ca > Mg and Zn > Fe > Cu > Mn > Se > Ni > Mo > Co, respectively. Cd and Pb levels in crab muscle tissue were determined to be lower than the limit values given in codices independent of the seasonal variables. However, as the levels were found to be above the limit values, metal concentrations in crabs were also evaluated based on provisional tolerable daily intake and provisional tolerable weekly intake limits.     

Impact of exogenous salicylic acid on the growth,antioxidant activity and physiology of carrot plants subjected to combined salinity and boron toxicity

Previous studies have shown that salicylic acid (SA) plays a role in the response of plants to salt and osmotic stresses. Therefore, an experiment was conducted to investigate the impact of exogenous salicylic acid on the growth, physiology and antioxidant activity of carrot (Daucus carota L. cv. Nantes) grown under combined stress of salinity and boron toxicity. The treatments consisted of salt (control, NaCl, and Na₂SO₄), boron (−B: 0 and +B: 25 mg B kg⁻¹) and salicylic acid (−SA: 0 and +SA: 0.5 mmol SA kg⁻¹). The diameter of the storage root was increased by NaCl salinity in the absence of B toxicity, however, it was increased by Na₂SO₄ salinity under B toxicity. For the storage root yield, NaCl salinity was more toxic than Na₂SO₄ salinity. With its role in plant growth regulation, SA application positively affected the storage root dry weight, S concentration, carotenoids and anthocyanin content and increased the total antioxidant activity (AA) of the shoot and storage root. SA application regulated proline and toxic ion (B, Cl) accumulation in the storage root and shoot. This study reports the long term effects of SA under stress conditions and reveals that SA was not as effective as in alleviating abiotic stress as reported in the literature conducted with short-term studies. That means long-term effects of SA would be significantly different from its short-term effects.     

Forest pest control by common redstart (Phoenicurus phoenicurus L.) near Antalya, Turkey

This research was conducted in two Mediterranean forest study plots (Bük-Lütfi Büyükyıldırım BL and Elmalı Cedarforest EZ) near Antalya, Turkey, between 2000 and 2002. Adult redstarts fed their nestlings exclusively with insects (79.5%). Three hundred and thirty seven prey items were identified of which 34.4% were Coleoptera, 17.2% Lepidoptera, 11.6% Diplopoda, 9.8% Diptera, 8.0 % Hymenoptera, 5% Heteroptera, 4.7% Arachnida, 2.7% Orthoptera, 1.8% Myriapoda, 1.2% zu Sedantaria, 0.6% each scorpions, Homoptera, and Dermaptera, and 0.3% each Chilopoda, Neuroptera, and earthworms (Lumbricus terrestris). Fourteen different forest pest species were identified of which seven species were Lepidoptera, four Coleoptera, two Hymenoptera and one Orthoptera.     

SNP allele frequency estimation in pooled DNA of Frankliniella occidentalis (Pergande, 1895) (Thysanoptera: Thripidae) by next-generation sequencing

Journal of Plant Diseases and Protection - Next-generation sequencing combined with bioinformatic analysis has become an instrumental tool for quick and reliable SNP discovery in genomes. Here, we...