Thrombocyte aggregation in the cat

Platelet aggregation in healthy and sick cats after adding various aggregating agents is described. Feline platelets aggregate irreversibly in response to 0.15-1.0 micrograms/ml collagen, 1 microM ADP, 0.3 IU/ml test-thrombin and 0.71 NIH/ml Topostasin. Epinephrine, ristocetin and kaolin failed to cause aggregation. The aggregation function was decreased in a cat with liver damage and icterus; in 2 cats with uremia platelet aggregation was normal. Acetylsalicylic acid (ASA) (10 mg/kg iv) inhibits platelet aggregation in the presence of collagen in low concentrations; high concentrations of collagen succeeded in inducing platelet aggregation.     

Measurement of nitrate leaching losses from arable plots under different nitrogen input regimes

Abstract. Leaching losses of nitrate-nitrogen were measured from a set of eight hydrologically isolated plots on a clay loam soil over the period from September 1987 to February 1990. Variable drainflow recovery from the plots hampered accurate estimation of nitrate loading, but results suggest that, when inorganic nitrogen fertilizer is applied up to the recommended amount, there is little influence of the amount applied on the amount leached. We did, however, observe the following effects on nitrate leaching: leguminous green manure incorporated in autumn increased leaching of nitrate-nitrogen by 10–15 kg per hectare during the winter; autumn cultivation caused some increase in leaching compared with no cultivation in one year; some systematic variations in nitrate leaching occurred between years and between plots, but were unrelated to treatments.
From the results we conclude that green manuring does not provide sufficient nitrogen for organically grown crops on this soil but contributes significantly to nitrate leaching, and that growing spring cereals, with the land remaining in stubble as long as possible in autumn, may be the best strategy to minimize nitrate leaching.     

THE INSENSITIVITY OF 99mTc PERTECHNETATE FOR DETECTING METASTASES OF A FUNCTIONAL THYROID CARCINOMA IN A DOG

This report describes the use of ^99mtechnetium pertechnetate (^99mTcO₄⁻) and ¹³¹[for imaging of a metastatic thyroid carcinoma in a dog. The ¹³¹] imaging showed metastatic lesions undetected by the ^99mTcO₄⁻ imaging on 2 separate occasions. The possible mechanisms for the discrepancies between ¹³¹I and ^99mTcO₄⁻ imaging of thyroid carcinomas are discussed. The use of ¹³¹I for the imaging of functional thyroid carcinomas in the dog is recommended.     

Purification and characterization of hepatic triacylglycerol lipase isolated from rainbow trout,Oncorhynchus mykiss

Trialcylglycerol (TG) lipase was isolated and partially purified from rainbow trout liver. Triacylglycerol lipase activity was assayed by measuring¹⁴C-oleic acid release from¹⁴C-triolein.¹⁴C-oleic acid release was linear for up to two hours. Optimal activity occurred at pH 7.0 and 15°C. Most of the lipase activity was recovered in the cytosolic fraction. A 27,000-fold purification was achieved after Sepharose (Bio-gel A 0.5 M, 200–400 mesh) chromatography of a resuspended 20% ammonium sulfate fraction. The molecular weight of the trout hepatic lipase as determined by size-exclusion chromatography and by SDS-polyacrylamide gel electrophoresis was 40–43 kD. Lipase-mediated hydrolysis of TG resulted in the production of diacylglycerols, monoacylglycerols, and fatty acids. Kinetic analysis indicated that V_max=0.016 nmol/h/mg protein and that K_m=0.28 mM triolein. Lipolytic activity was enhanced in the presence of cAMP/ATP-Mg²⁺. These results suggest that the liver of trout possesses a neutral TG lipase that is responsible for mobilizing stored TG and is catalytically activated by phosphorylation.A part of this work was presented at the Annual Meeting of the American Society of Zoologists, December 26–30, 1990, San Antonio, TX.     

Influence of Low Light Irradiance on Grain Filling in Rice (Oryza saliva L.) cultivars

Time course of grain filling pattern in two rice (Oryza sativa L.) cultivars, grown under normal and low light intensities, was studied. The number of spikelets, partially filled grains and high density grains were monitored at a 5 day interval during grain filling period from uniform panicles tagged at anthesis. The low light tolerant cultivar Swarnaprabha had more high density grains and less partially filled grains throughout the grain filling period and at harvest compared to cultivar Ratna under both normal and low light intensities. Further, the opening of spikelets in two flushes in Swarnaprabha seemed to result in a more efficient resource allocation and hence better yield as compared to Ratna, in which the opening of spikelets was in a single flush on day 10.     

Metal toxicosis in horses.

The ubiquity and stability of metals in the environment make them unique as a pollutant or an essential dietary component. Metals are neither created nor destroyed by chemical processes but are redistributed in the environment. In combination with other elements, metal compounds and alloys are essential materials of the contemporary world. Inappropriate use or distribution in the environment leads to adverse health effects on all biologic systems, including horses. Gastrointestinal upset is a common feature of acute toxicosis with metals in general. Among the metals discussed, arsenic and inorganic mercury have a propensity to do severe damage to the gut. Deposition of cadmium on forage is the source most likely to intoxicate horses. This subchronic to chronic problem in horses is manifest as disease of the musculoskeletal system and kidneys. Iron-containing hematinics are widely used in racetrack horses and occasionally result in hepatopathy when excessive doses are administered. Lead continues to be recognized as the most significant environmental metal pollutant. Poisoning is encountered routinely in humans and animals. Of the animal species of veterinary concern, lead-poisoned horses are not a frequent encounter. Lead-intoxicated horses show signs of peripheral neuropathy (laryngeal hemiplegia), intermittent colic, and mild anemia. Acute mercury poisoning sometimes occurs from the common use of mercury-containing blistering agents, with most clinical findings related to acute renal failure. Chronic excessive intake of zinc by horses is uncommon but devastating in rapidly growing foals. The mechanism of chronic zinc toxicosis is coupled to the induced copper deficiency. The condition is a disease of cartilage in the articular and growth physes.     

Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.     

Comparison of sesame cake and cottonseed as supplementary sources of protein to weaned N'Dama bull calves in The Gambia

Summary The potential for increased productivity of N'Dama cattle in response to small supplementary inputs of proteinaceous by-products has been shown to be substantial under village husbandry conditions in The Gambia. Young N'Dama bulls were used to compare sesame cake with cottonseed as sources of supplementary protein during the wet and early dry seasons of 1987. They were fed 0, 40, 80 or 160 g crude protein/day in these forms for four months. Supplementation significantly increased growth rate from 169 g/d in controls to means of 272 and 271 g/d for those given sesame cake and cottonseed respectively; there were no significant differences among the supplemented groups. During the four months of the dry season immediately after supplementation ceased all previously supplemented groups grew significantly more rapidly than the controls (at leastP<0·05), indicating a substantial carry over effect of the supplements. Over this period the mean gain of the groups previously given sesame cake exceeded that of those that had received cottonseed (217 cf. 158 g/d;P<0·01). The economic benefits of these responses were substantial.

---

Comparacion de torta de sesamo y de semilla de algodon como fuente suplementaria de proteina para terneros destetos N'Dama en Gambia

Resumen Se ha demonstrado el potencial para incrementar la productividad del ganado N'Dama, como resquesta a peque?as cantidades de insumos suplementarios de proteína, bajo condiciones de villorrio en Gambia. Se utilizaron terneros destetos N'Dama par comparar la torta de sésamo y de semilla de algodón como fuente de proteína suplementaria durate la estaicón lluviosa y principio de la seca del a?o 1987. Se les dió a los animales 0, 40, 80 o 160 g de proteína cruda/día en éstas formas durante cuatro meses. La suplementación incrementó significativamente la tasa de crecimiento, de 169 g/día en controles a 272 y 271 g/día en aquellos que recibieron torta de sésamo y de algodón respectivamente; no hubo diferenca estadística entre los grupos suplementados. Durate los cuatro meses de la estación seca inmediatamente después de que la suplementación fue suspendida, todos los grupos que habían sido suplementados crecieron sgnificativamente más rápido que los controls (P<0·05), indicando un efecto compensatorio en los suplementados. Durante ese período la media de crecimento de los grupos que recibieron torta de sésamo excedió a aquella de los que habían recibido torta de algodón (217 cf. 158 g/d;P<0·01). Los beneficios económicos de estos resultados fueron substanciales.

---

Comparaison entre le tourteau de sesame et la graine de coton comme sources complementaires de proteines pour les taurillons N'Dama sevres en Gambie

Résumé Le potentiel de réponse à de petites quantités supplémentaires de sous-produits proteiniques pour accroitre la productivité de bétail N'Dama, s'est révélé manifeste, dans les conditions d'élevage villageois en Gambie. De jeunes taureaux N'Dama ont été utilisés pour comparer le tourteau de sésame et la graine de coton en tant que source de complémentation protéinique pendant la saison sèche et le début de la saison humide au cours de l'année 1987. Ils ont re?u respectivement 0, 40, 80 ou 160 g de proteines brutes par jour sus ces deux formes pendat quatre mois. Cette supplémentation a accru de fa?on significative le taux de croissance, de 169 g/jour pour les témoins jusqu'à des moyennes de 272 et 271g/jour respectivement pour ceux qui ont re?u du tourteau de sésame et de la graine de coton. Quant aux groupes complémentés, il n'ont révélé aucune différence significative. Pendat les quatre mois de la saison sèche et immédiatement après l'arrêt de la supplémentation, tous les lots ayant fait précédemment l'objet d'une distribution complémentaire ont eu une croissance significativement plus rapide que les animaux témoins, du moins pourP<0,05, ce qui indique un effet important de report des suppléments. Durant cette période, le gain moyen des lots qui ont re?u le tourteau de sésame a dépassé celui des lots auxquels on a donné de la graine de coton, soit 217 g/jour contre 158 g/jour pourP<0,01. Les profits économiques de ces réponses ont été appréciables.