Factors causing variation in fine root biomass in forest ecosystems

Fine roots form one of the most significant components contributing to carbon cycling in forest ecosystems. We study here the effect of variation in root diameter classes, sampling depth and the inclusion of understorey vegetation root biomass in fine root biomass (FRB) estimates. The FRB estimates for different forest biomes are updated using a database of 512 forest stands compiled from the literature. We also investigate the relationships between environmental or forest stand variables and fine root biomass (≤2 mm in diameter) at the stand (g m⁻²) and tree level (g tree⁻¹). The FRB estimates extrapolated for the whole rooting depth were 526 ± 321 g m⁻², 775 ± 474 g m⁻² and 776 ± 518 g m⁻² for boreal, temperate and tropical forests, respectively, and were 26-67% higher than those based on the original sampling depths used. We found significant positive correlations between ≤1 and ≤2 mm diameter roots and between ≤2 and ≤5 mm roots. The FRB estimates, standardized to the ≤2 mm diameter class, were 34-60% higher and 25-29% smaller than those standardized to the ≤1 mm and ≤5 mm diameter classes, respectively. The FRB of the understorey vegetation accounted for 31% of the total FRB in boreal forests and 20% in temperate forests. The results indicate that environmental factors (latitude, mean annual precipitation, elevation, temperature) or forest stand factors (life form, age, basal area, density) can not explain a significant amount of the variation in the total FRB and a maximum of 30% that in the FRB of trees at the stand level, whereas the mean basal area of the forest stand can explain 49% of the total FRB and 79% of the FRB of trees at the tree level.     

Carbon sequestration in the growing stock of trees in Finland under different cutting and climate scenarios

In this work the aim was to determine how carbon sequestration in the growing stock of trees in Finland is dependent on the forest management and increased production potential due to climate change. This was analysed for the period 2003–2053 using forest inventory data and the forestry model MELA. Four combinations of two climate change and two management scenarios were studied: current (CU) and gradually warming (CC) climate and forest management strategies corresponding to different rates of utilisation of the cutting potential, namely maximum sustainable removal (Sust) or maximum net present value (NPV) of wood production (Max). In this analysis of Finland, the initial amount of carbon in the growing stock was 765 Mt (2,802 Tg CO₂). At the end of the simulation, the carbon in the growing stock of trees in Finland had increased to 894 Mt (3,275 Tg CO₂) under CUSust, 906 Mt (3,321 Tg CO₂) under CUMax, 1,060 Mt (3,885 Tg CO₂) under CCSust and 1,026 Mt (3,758 Tg CO₂) under CCMax. The results show that future development of carbon in the growing stock is not only dependent on climate change scenarios but also on forest management. For example, maximising the NPV of wood production without sustainability constraints results, over the short term, in a large amount of wood obtained in regeneration cuttings and a consequent decrease in the amount of carbon in growing stock. Over the longer term, this decrease in the carbon of growing stock in regenerated forests is compensated by the subsequent increase in fast-growing young forests. By comparison, no drastic short-term decrease in carbon stock was found in the Sust scenarios; only minor decreases were observed.     

Seed set of ulluco (Ullucus tuberosus Loz.). Variation between clones and enhancement of seed production through the application of plant growth regulators

Summary Ulluco, Ullucus tuberosus, is a tuber crop plant with a reduced ability to produce botanical seed. This paper discusses problems related to the botanical seed set of ulluco. Ten clones of this species were studied, and the number of seeds per plant was recorded. Enhancement of seed production was attempted by the application of three growth regulators; gibberellic acid, CCC, and a synthetic cytokinin, 6-benzylamine purine. Seed set ability was shown to vary between the clones, and the application of plant growth regulators did not enhance seed production. Variation in fruit weight was observed. Because of genetic variation between clones in their ability to set seed, it is proposed that there is a possibility of breeding ulluco using botanical seeds. However, methods for improving the yield of botanical seed need to be developed.     

Brook Water Quality and Background Leaching from Unmanaged Forested Catchments in Finland

The water chemistry of 21 outlet brooks in undisturbed, forested catchments (0.074–38 km²) in Finland was monitored during three years (1997–1999) in order to estimate the background levels and leaching of total organic carbon (TOC), iron, nitrogen and phosphorus, and examine the relationships between catchment characteristics and brook water quality in pristine forested areas. The studied catchments are located throughout Finland except the northernmost parts of the country. The concentrations of TOC were relatively high, on average 20 mg L^-1. Annual leaching of TOC ranged from 3000 to 10 000 kg km^-2. The average total nitrogen concentration and annual leaching were 430 μg L^-1 and 140 kg km^-2, respectively. The average total phosphorus concentration and annual leaching were 15 μg L^-1 and 5.4 kg km^-2, respectively. On a national level temperature and discharge conditions were the most important variables for predicting total phosphorus, total nitrogen and TOC concentrations. Both total nitrogen and total phosphorus concentrationsdisplayed positive correlation with temperature, and thus the concentrations were higher in the south than in the north. Nitrogen was mainly organic and showed strong correlation with TOC. On the catchment level, both TOC and total nitrogen concentrations and export had a strongpositive correlation with the abundance of Norway spruce (Piceaabies Karsten) and a strong negative correlation with the abundance of Scots pine (Pinus sylvestris L.). Nitrate concentrations and leaching were related to average site type. The more fertile the average site type was in the catchment, the higher the nitrate concentrations and export were.     

Acute phase response in two consecutive experimentally induced E. coli intramammary infections in dairy cows

Background

Acute phase proteins haptoglobin (Hp), serum amyloid A (SAA) and lipopolysaccharide binding protein (LBP) have suggested to be suitable inflammatory markers for bovine mastitis. The aim of the study was to investigate acute phase markers along with clinical parameters in two consecutive intramammary challenges with Escherichia coli and to evaluate the possible carry-over effect when same animals are used in an experimental model.

Methods

Mastitis was induced with a dose of 1500 cfu of E. coli in one quarter of six cows and inoculation repeated in another quarter after an interval of 14 days. Concentrations of acute phase proteins haptoglobin (Hp), serum amyloid A (SAA) and lipopolysaccharide binding protein (LBP) were determined in serum and milk.

Results

In both challenges all cows became infected and developed clinical mastitis within 12 hours of inoculation. Clinical disease and acute phase response was generally milder in the second challenge. Concentrations of SAA in milk started to increase 12 hours after inoculation and peaked at 60 hours after the first challenge and at 44 hours after the second challenge. Concentrations of SAA in serum increased more slowly and peaked at the same times as in milk; concentrations in serum were about one third of those in milk. Hp started to increase in milk similarly and peaked at 36–44 hours. In serum, the concentration of Hp peaked at 60–68 hours and was twice as high as in milk. LBP concentrations in milk and serum started to increase after 12 hours and peaked at 36 hours, being higher in milk. The concentrations of acute phase proteins in serum and milk in the E. coli infection model were much higher than those recorded in experiments using Gram-positive pathogens, indicating the severe inflammation induced by E. coli.

Conclusion

Acute phase proteins would be useful parameters as mastitis indicators and to assess the severity of mastitis. If repeated experimental intramammary induction of the same animals with E. coli is used in cross-over studies, the interval between challenges should be longer than 2 weeks, due to the carry-over effect from the first infection.