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Demonstration of a new intracellular antigen in porcine intestinal adenomatosis and hamster proliferative ileitis 总被引:6,自引:0,他引:6
In the proliferate enteropathies of the pig, intracellular Campylobacter-like organisms can be demonstrated in the apical cytoplasm of affected cells. A new antigen (omega) can be visualised at the site of bacterial parasitism using certain rabbit sera in a sandwich immunofluorescence test; the rabbit sera tested were largely derived from rabbits immunised with bacterial antigens including Campylobacter spp., but it appeared that the reaction was not related to the immunisation procedure and was also present in at least one "normal" rabbit serum. Rabbits immunised with bacteria extracted from the tissues sero-converted to omega-antigen yet did not react with Campylobacter spp., cultured from pig intestine. The omega-antigen was also present in the lesions of hamster proliferative ileitis. 相似文献
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C. Lawson S.A. Smith M. O'Brien M. McMichael 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):128-134
Background
Neutrophil extracellular traps (NETs) are part of the innate immune response and are essential in local pathogen control, but are associated with pathological inflammation, organ damage, autoimmunity, and thrombosis. Immune‐mediated hemolytic anemia (IMHA) is a pro‐inflammatory, prothrombotic disease associated with high mortality.Hypothesis/Objectives
Neutrophil extracellular traps (NETs) are a feature of the inflammatory process in dogs with IMHA. The objective of the study was to evaluate plasma from dogs with IMHA for the presence of 2 indirect markers and 1 direct marker of NETs.Animals
Healthy client‐owned dogs (56) and hospitalized dogs with IMHA (n = 35).Methods
Prospective study. Plasma samples for all dogs were evaluated for cell‐free DNA using a fluorescence assay, histone‐DNA (hisDNA) complex using an ELISA, and citrullinated histone H3 (specific for NETosis) using Western blot. Reference intervals were generated using plasma from healthy dogs.Results
In dogs with IMHA, cell‐free DNA concentration was above the reference interval in 17% of samples with a median (range) of 1.0 μg/mL (0.1–17.3), and hisDNA concentration was above the reference interval in 94% of samples with a median (range) of 30.7 × pooled normal plasma (PNP; 0.6–372.1). Western blot for citrullinated histone H3 identified detectable bands in 84% samples from dogs with IMHA.Conclusions and Clinical Importance
The assay for cell‐free DNA detected evidence of NETs in fewer dogs than did the other approaches. Excessive NETs appears to be a feature of IMHA in dogs and contributions to the prothrombotic state deserve further study. 相似文献106.
Sita S. Withers Corinne M. Lawson Andrew G. Burton Robert B. Rebhun Michele A. Steffey 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(3):299-304
We describe the surgical and post-operative management of a large, invasive, and metastatic functional Sertoli cell tumor in a 9-year-old cryptorchid male Labrador retriever dog. Despite residual disease after surgery, bone marrow recovery occurred without administration of bone marrow stimulants and serum estradiol accurately predicted tumor recurrence. 相似文献
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Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line. 总被引:3,自引:2,他引:3 下载免费PDF全文
K F Lawson R Hertler K M Charlton J B Campbell A J Rhodes 《Canadian journal of veterinary research》1989,53(4):438-444
The ERA strain of rabies virus was propagated in a baby hamster kidney cell line (BHK-21/C13). The viral titer was 10(1.8) tissue culture infective doses (TCID) higher than that of commercial ERA vaccine. The ERA/BHK-21 vaccine in baits retained titers of 10(6.3) to 10(6.4), TCID when subjected to daily temperature fluctuations from 9 degrees C to 24 degrees C for 21 days. This titer, according to a dose response in laboratory foxes, was still capable of immunizing up to 100% of foxes consuming a bait. The ERA/BHK-21 vaccine, when presented in baits, produced antibodies in 80 to 100% of dogs consuming more than one bait. Duration of immunity in foxes, from feeding the ERA strain rabies virus in baits, as determined by resistance to challenge with virulent virus, was at least 48 months. The vaccine strain retained some pathogenicity for nontarget species. In tests carried out on foxes, raccoons, dogs, cats and cattle, the vaccine did not cause vaccine-induced rabies. One of 14 skunks which consumed four baits developed vaccine-induced rabies, but virus could not be isolated from the salivary glands of this animal. The vaccine, when presented in baits, caused vaccine-induced rabies in 37% of laboratory mice, 3.4% of Microtus and 2.6% of Peromyscus species. Rabies virus could not be isolated from the salivary glands of rodents with vaccine-induced rabies. It was concluded that ERA virus propagated in BHK-21/C13 cells and incorporated in an acceptable bait produced a high titer, stable, immunogenic and safe vaccine for foxes. 相似文献
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