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141.
Surface waters of the subtropical Sargasso Sea contain dissolved inorganic phosphate (DIP) concentrations of 0.2 to 1.0 nanomolar, which are sufficiently low to result in phosphorus control of primary production. The DIP concentrations in this area (which receives high inputs of iron-rich dust from arid regions of North Africa) are one to two orders of magnitude lower than surface levels in the North Pacific (where eolian iron inputs are much lower and water column denitrification is much more substantial). These data indicate a severe relative phosphorus depletion in the Atlantic. We hypothesize that nitrogen versus phosphorus limitation of primary production in the present-day ocean may be closely linked to iron supply through control of dinitrogen (N2) fixation, an iron-intensive metabolic process. Although the oceanic phosphorus inventory may set the upper limit for the total amount of organic matter produced in the ocean over geological time scales, at any instant in geological time, oceanic primary production may fall below this limit because of a persistent insufficient iron supply. By controlling N2 fixation, iron may control not only nitrogen versus phosphorus limitation but also carbon fixation and export stoichiometry and hence biological sequestration of atmospheric carbon dioxide. 相似文献
142.
Gaffen DJ Santer BD Boyle JS Christy JR Graham NE Ross RJ 《Science (New York, N.Y.)》2000,287(5456):1242-1245
Trends in global lower tropospheric temperature derived from satellite observations since 1979 show less warming than trends based on surface meteorological observations. Independent radiosonde observations of surface and tropospheric temperatures confirm that, since 1979, there has been greater warming at the surface than aloft in the tropics. Associated lapse-rate changes show a decrease in the static stability of the atmosphere, which exceeds unforced static stability variations in climate simulations with state-of-the-art coupled ocean-atmosphere models. The differential temperature trends and lapse-rate changes seen during the satellite era are not sustained back to 1960. 相似文献
143.
Laura B. DeLind 《Agriculture and Human Values》1987,4(2-3):4-10
The deepening U.S. farm crisis has been accompanied by numerous benefit fund raisers, individual donations and volunteer programs—all an expression of cooperation and concern on the part of U.S. citizens, farmer and non-farmer alike. These responses have received wide media attention and much public praise. A sense of patriotism and self-reliance underlies their popularity. Nevertheless, such efforts work to undermine their own ultimate objective—that of improving the economic circumstances of the family farm and farm family. This irony, it is argued, arises from the fact that these charitable responses typically depoliticize the nature of the U.S. farm crisis. First, they deflect public attention away from the larger economic context and the structural inequities within it. Second they revitalize agrarian-based myths which serve to rationalize and to reproduce the ‘independent’ behavior of small, commercial farmers. Finally, it is argued that the depoliticization of the farm crisis is itself a political strategy, one which supports and legitimizes ‘business as usual’ and is compatible with the interests of corporate agriculture. 相似文献
144.
Substrate stabilization: genetically controlled reciprocal relationship of two human enzymes 总被引:8,自引:0,他引:8
5-Phosphoribosyl-l-pyrophosphate, a substrate shared by adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase, accumulates in human erythrocytes lacking hypoxanthine-guanine phosphoribosyltransferase. 5-Phosphoribosyl-l-pyrophosphate added to purified adenine phosphoribosyltransferase stabilizes it against heat inactivation. The increased activity of adenine phosphoribosyltransferase seen in erythrocytes deficient in hypoxanthine-guanine phosphoribosyltransferase may result from substrate stabilization of this enzyme in vivo. 相似文献
145.
Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine 总被引:12,自引:0,他引:12
W J Burrows D J Armstrong F Skoog S M Hecht J T Boyle N J Leonard J Occolowitz 《Science (New York, N.Y.)》1968,161(842):691-693
We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine, C(16)H(23)N(5)0(4)S, on the basis of low-and high-reso!ution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical. 相似文献
146.
Variations in the cadmium/calcium ratio of North Atlantic Deep Water are recorded in the fossil shells of benthic foraminifera. The oceanic distribution of cadmium is similar to that of the nutrients, hence the cadmium/calcium ratio in shells records temporal variations in nutrient distributions. Data from a North Atlantic sediment core show that over the past 200,000 years there has been a continuous supply of nutrient-depleted waters into the deep North Atlantic. The intensity of this source relative to nutrient-enriched southern waters diminished by about a factor of 2 during severe glaciations. This evidence combined with carbon isotope data indicates that the continental carbon inventory may have been less variable than previously suggested. 相似文献
147.
Kochendoerfer GG Chen SY Mao F Cressman S Traviglia S Shao H Hunter CL Low DW Cagle EN Carnevali M Gueriguian V Keogh PJ Porter H Stratton SM Wiedeke MC Wilken J Tang J Levy JJ Miranda LP Crnogorac MM Kalbag S Botti P Schindler-Horvat J Savatski L Adamson JW Kung A Kent SB Bradburne JA 《Science (New York, N.Y.)》2003,299(5608):884-887
We report the design and total chemical synthesis of "synthetic erythropoiesis protein" (SEP), a 51-kilodalton protein-polymer construct consisting of a 166-amino-acid polypeptide chain and two covalently attached, branched, and monodisperse polymer moieties that are negatively charged. The ability to control the chemistry allowed us to synthesize a macromolecule of precisely defined covalent structure. SEP was homogeneous as shown by high-resolution analytical techniques, with a mass of 50,825 +/-10 daltons by electrospray mass spectrometry, and with a pI of 5.0. In cell and animal assays for erythropoiesis, SEP displayed potent biological activity and had significantly prolonged duration of action in vivo. These chemical methods are a powerful tool in the rational design of protein constructs with potential therapeutic applications. 相似文献
148.
Egloff S O'Reilly D Chapman RD Taylor A Tanzhaus K Pitts L Eick D Murphy S 《Science (New York, N.Y.)》2007,318(5857):1777-1779
RNA polymerase II (Pol II) transcribes genes that encode proteins and noncoding small nuclear RNAs (snRNAs). The carboxyl-terminal repeat domain (CTD) of the largest subunit of mammalian RNA Pol II, comprising tandem repeats of the heptapeptide consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expression of both gene types. We show that mutation of serine-7 to alanine causes a specific defect in snRNA gene expression. We also present evidence that phosphorylation of serine-7 facilitates interaction with the snRNA gene-specific Integrator complex. These findings assign a biological function to this amino acid and highlight a gene type-specific requirement for a residue within the CTD heptapeptide, supporting the existence of a CTD code. 相似文献
149.
Helmuth L 《Science (New York, N.Y.)》2002,297(5582):752-753
150.