Insect resistance in potatoes: sources,evolutionary relationships,morphological and chemical defenses,and ecogeographical associations

Summary The past 25 years, 1686 potato accessions, representing 100 species in the genus Solanum L., subgenus Potatoe, section Petota, were evaluated for field resistance to one or more of the following insect pests: green peach aphid, Myzus persicae (Sulzer); potato aphid, Macrosiphum euphorbiae (Thomas); Colorado potato beetle, Leptinotarsa decemlineata (Say); potato flea beetle, Epitrix cucumeris (Harris); and potato leafhopper, Empoasca fabae (Harris). Accessions highly resistant to green peach aphid were identified within 36 species, to potato aphid within 24 species, to Colorado potato beetle within 10 species, to potato flea beetle within 25 species, and to potato leafhopper within 39 species. Resistance levels were characteristic within Solanum species. Insect resistance appears to be a primitive trait in wild potatoes. Susceptibility was most common in the primitive and cultivated Tuberosa. Insect resistance was also characteristic of the most advanced species. The glycoalkaloid tomatine was associated with field resistance to Colorado potato beetle and potato leafhopper. Other glycoalkaloids were not associated with field resistance at the species level. Dense hairs were associated with resistance to green peach aphid, potato flea beetle, and potato leafhopper. Glandular trichomes were associated with field resistance to Colorado potato beetle, potato flea beetle, and potato leafhopper. Significant correlations between insect score and altitude of original collection were observed in six of thirteen species. Species from hot and arid areas were associated with resistance to Colorado potato beetle, potato flea beetle, and potato leafhopper. Species from cool or moist areas tended to be resistant to potato aphid.Abbreviations EBN Endosperm Balance Number     

Pharmacokinetics of intravenously and orally administered pyrimethamine in horses.

Single-dose pharmacokinetic variables of pyrimethamine were studied in horses. Pyrimethamine (1 mg/kg of body weight) was administered IV and orally to 6 adult horses, and plasma samples were obtained at frequent intervals thereafter. Plasma pyrimethamine concentration was assayed by gas chromatography, and concentration-time data were analyzed, using a pharmacokinetic computer program. The IV and oral administration data were best described by 3-compartment and 1-compartment models, respectively. The median volume of distribution at steady state after IV administration was 1,521 ml/kg and the median elimination half-time was 12.06 hours. Mean plasma concentration after oral administration fluctuated between a maximal concentration of 0.18 microgram/ml and 0.09 microgram/ml (24 hours after dosing). Bioavailability after oral administration was 56%.     

Evaluation of the use of subcutaneous implantable vascular access ports in feline blood donors

OBJECTIVE:To compare the ease and effects of collecting blood from cats by use of subcutaneous totally implantable vascular access ports (VAPs) with collection via conventional jugular phlebotomy. DESIGN: Prospective randomized experimental study. ANIMALS: 8 healthy cats. PROCEDURES: Cats in the port group (n = 4) underwent monthly blood donation by use of VAPs and manual restraint, and cats in the nonport group (4) underwent monthly blood donation by use of conventional jugular phlebotomy and sedation, for 6 months. RESULTS: Postsurgical VAP-related complications developed in 3 cats and included port erosion (n = 1), disconnection of the port from the catheter (1), and seroma formation (1). Blood was successfully collected 24 of 24 and 20 of 20 times in the nonport and port groups, respectively. Results of bacterial culture of blood were negative in 22 of 24 and 15 of 20 nonport and port collections, respectively. No differences in RBC morphology were observed between groups. Mean blood collection and total donation times were significantly longer for the nonport group. Collection time was more variable in the nonport group, and cats were less tolerant of handling during venipuncture, compared with cats in the port group. Blood collection required a mean of 2.4 persons for the nonport group and 2.1 persons for the port group. CONCLUSIONS AND CLINICAL RELEVANCE: Positive results for blood collections via VAPs were increased donor acceptance, decreased number of personnel required, and decreased collection time. Drawbacks included contamination of blood products and port-related complications.     

Frequency of 2n pollen in diploid hybrids betweenSolanum phureja Juz. & Buk. andSolanum chacoense Bitt.

Summary Solanum phureja (PP)xSolanum chacoense (CC) hybrid families (PC) were evaluated for pollen fertility and frequency of 2n pollen in two seasons in Morocco. PP clones produced high frequencies of 2n pollen, whereas CC clones produced none. The percentage of plants with 2n pollen ranged from 2 to 63% among 20 PC families and was consistent in some PC families over two growing seasons, whereas others varied between fall and spring growing seasons. The expected ratio of 2n to non-2n pollen-producers within a PC family based on a single recessive gene model [i.e. 1∶0 with CC homozygous dominant (PsPs) or 1∶1 with CC heterozygous (Psps)] was observed in only 5 of 20 progenies. Narrow sense heritability was 0.71, allowing gain from further selection. Only clones producing more than 10% 2n pollen exhibited more stable expressivity across seasons. Scientific Journal Series Article 17811 of the Minnesota Agricultural Experiment Station supported in part by the US Agency for International Development, contract no. 608-0160, IAV Morocco Project.     

Krantz: A Russet cultivar for the irrigated sands

Krantz is an oblong to slightly blocky, russet-skinned potato cultivar with medium-late maturity and a vigorous vine. It is resistant to hollow heart, has high resistance to common scab and possesses high field resistance to late blight. It is moderately resistant toVerticillium wilt, susceptible to early blight, viruses S and X, and exhibits symptoms of bacterial ring rot. Krantz is adapted to the irrigated sands, but will growth crack in heavier soils.     

Early detection of Verticillium wilt resistance in a potato breeding program

Nine potato clones/cultivars selected for varying degrees of Verticillium wilt (VW) resistance based on degree of vascular colonization, were intercrossed and outcrossed to 13 uncharacterized clones. Based on growth characteristics of 3,535 seedling transplants and an assay quantifyingV. dahliae in potato vascular tissue, 404 clones were selected for further tests. The average number ofVerticillium dahliae colony forming units (CFU) expressed as Log₁₀(CFU + l)/0.1 ml of plant sap for the 404 clones was 2.3, and 1.3 and 3.7 for resistant Reddale and susceptible Kennebec checks, respectively. Thirty-five clones (8.66%) had CFU values equal to or less than Reddale suggesting that VW resistance is a readily transmitted trait.     

Effect of environment and direction of hybridization on genetic variability in two diploid potato populations

Summary Twelve selections from each of theSolanum tuberosum Groups haploid Tuberosum and Phureja were intercrossed in a NC Design II pattern to generate reciprocal hybrids. Yield parameters, combining ability and variance components were estimated from field experiments conducted at three locations, one in Minnesota and two in Morocco. Heterosis for yield and tuber number was significant at all locations, but only in Tuberosum cytoplasm. Estimates of general combining ability (GCA) for Tuberosum parents and specific combining ability (SCA) for Tuberosum-Phureja combinations varied with location and direction of hybridization. GCA of Phreja parents, however, was consistently significant at all locations and in both cytoplasms. Genetic variance estimates revealed predominantly non-additive gene action for most traits. Environment and direction of hybridization significantly influenced estimates, of variance components, indicating that hybrid performance should be evaluated in both cytoplasms and in the target environments for effective utilization of this germplasm in future breeding efforts. Scientific Journal Series Article 14,991 of the Minnesota Agricultural Experiment Station supported in part by the U.S. Agency for International Development, contract no. 608-0160, IAV Morocco Project.