Response of Nooksack potatoes to nitrogen fertilizer

The Nooksack cultivar because of differing characteristics may have different responses to grower management practices developed principally for Russet Burbank. This study was conducted to determine the effects of nitrogen on yield, grade, and specific gravity of the Nooksack cultivar. In irrigated field experiments, N fertilizer was sprinkler-applied daily on Quincy sand soil. In other experiments, on a Warden silt loam soil all N was broadcast and incorporated before planting. Fertilization rate did not significantly affect tuber grade or specific gravity. Short term shifts in N supply did not cause tuber deformities and poor grades as they commonly do with Russet Burbank. Nooksack, while responsive, did not have a strong positive response to N fertilization. Tuber yields were depressed at fertilizer plus residual N levels above 200 kg/ha on Warden silt loam and above 300 kg/ha on Quincy sand. This difference was because of variation in NO₃ leaching and soil N mineralization and was reflected in calculation of optimum N rates. Nooksack may require less N fertilization than Russet Burbank partially because of the strong dormancy of Nooksack tubers that retards emergence and shortens growing season. Management to promote more rapid dormancy break and lower N nutrition during tuber initiation could result in earlier establishment, earlier tuber bulking, and greater yields of Nooksacks.     

Detection of the Matrix Metalloproteinases MMP‐2 and MMP‐9 and Tissue Inhibitors of Metalloproteinases TIMP‐1 and TIMP‐2 in Llama (Lama glama) Oviduct

Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are involved in several reproductive events like oocyte–spermatozoa interaction and semen liquefaction. In order to study their role in the llama oviductal reproductive process, MMP activity in oviductal fluid (OF) was assayed. Considering that llama genome sequences are partially known, a strategy to procure cDNA sequences of MMP‐2, MMP‐9, TIMP‐1 and TIMP‐2 was designed. Afterwards, their expression patterns in the different llama oviductal segments were assayed. Gelatine zymograms detected 62 and 94 kDa protease activities that matched MMP‐2 and pro‐MMP‐9, respectively. Expression pattern analysis showed that MMP and TIMP mRNAs were present in ampulla, isthmus, utero‐tubal junction (UTJ) and papilla. Altogether, these findings support the argument that MMPs/TIMPs are produced in the oviduct and secreted into the oviductal lumen. Our results encourage further studies to elucidate the role of these proteins in reproductive oviductal events.