Regional distribution of microsomal xenobiotic and steroid metabolism in kidney microsomes from rainbow trout

The regional distribution of microsomal cytochrome P-450-mediated reactions with exogenous and endogenous substrates in the kidney of rainbow trout was studied. The cytochrome P-450-dependent 7-ethoxyresorufin- and 7-ethoxycoumarin-0-deethylase activities were significantly higher (3–4 and 10–14 fold, respectively) in the trunk kidney than in the head kidney, whereas ethylmorphine-N-demethylase activity was evenly distributed along the kidney. The microsomal cytochrome P-450-dependent steroid hydroxylases and steroid reducing enzymes when using androstenedione as substrate also exhibited a regional distribution in trout kidney. The 6β- and 16-hydroxylase activities as well as the 5α-reductase and 17 hydroxysteroid oxidoreductase activities were higher in the anterior part of the trunk kidney than in the head kidney and posterior trunk kidney.     

Swedish Forest Owners’ Associations: Establishment and Development After the 1970s

The context under which forest owners’ associations operate has changed dramatically since the first ones were established in the 1910s and 1920s. The purpose of this article is to describe the development of these associations after their establishment particularly since the financial crises at the end of 1970s. The diffusion and merger theories form a conceptual base. The annual reports of the associations are the main data source. The number of forest owners’ associations after 1,985 decreased dramatically and presently is only four. The consolidation can be explained by a wish to achieve increased efficiency through economies of scale and more influence on the wood market. After the financial crises at the end of the 1970s the number of members dropped sharply but has since then increased. Some associations have had significant financial problems. In the 1970s it became apparent that during economic downturns the relatively low proportion of equity made them vulnerable. Return on equity has, on average, during the 2000s been around 7–8 %. One problem for the associations is the risk that larger and more efficient organisations lead to increased “distance” between the associations and their members. Another problem is that the members cannot take advantage of the large value growth. Moreover, there is capital tied up in operations that do not really have any formal ownership.     

Chemical treatment of lobster eggs against epibiotic bacteria

Eggs of the European lobster, Homarus gammarus (L.), were exposed to malachite green (5, 10, 15 mg 1^–1: 10 min), glutaraldehyde (50, 100, 150 mg 1^–1: 3 min) and iodine as Buffodine^TM (50, 100, 150 mg 1^–1: 10 min). The efficiency of the treatments was tested by incubating eggs individually in wells of multiwell dishes with TSB agar for 14 days after exposure. In order to find any effect on viability, batches of 30 eggs from each of three females were incubated artificially in a recirculation system for 19 days and repeatedly exposed to the disinfectants. Iodine as Buffodine (150 mg 1^–1) was the only treatment that resulted in a significant decrease of the bacterial growth on lobster eggs, but the treatment also resulted in inhibited hatching compared with the control group. Thus, our results indicate that treatment with 150mg^–1 iodine as Buffodine could be a strategy for reducing bacterial growth on lobster eggs when massive egg mortality due to bacteria is otherwise unavoidable. The treatment could, however, lead to decreased viability of larvae due to inhibited hatching.     

Ungulate-adapted forest management: effects of slash treatment at harvest on forage availability and use

Forest management strongly influences the interactions between ungulates and their food resources. Different ungulate-adapted measures have been proposed in forestry to improve forage availability or to reduce browsing damage. However, the potential and feasibility of such measures are inadequately known. We studied the effects of harvest timing and slash treatment in final felling and commercial thinning on the availability of Scots pine Pinus sylvestris forage and its use by ungulates during winter in the Swedish boreal forests. Pellet group counts showed that moose (Alces alces) was the dominating species using the post-harvest stands. Under conventional slash treatment, final felling stands held on average 226 kg pine forage ha^?1 after harvesting and commercial thinning stands 137 kg ha^?1. Ungulate-adapted slash treatment increased the available forage biomass by 20 %, but had no significant effect on consumption of forage by ungulates. Time since harvest had the strongest effect on forage consumption; for example, under conventional slash treatment, there was a tenfold increase in consumption (3 vs. 33 kg ha^?1) following final felling as exposure time increased from 2–3 to 4–5 months. Consumption was higher in thinned stands than in final felling stands for the first 3 months but not later. To increase ungulate use of the forage made available at harvest, pine-dominated stands should be harvested in the late autumn or early in the winter.     

Seed germination in weedy Brassica campestris and its hybrids with B. napus: Implications for risk assessment of transgenic oilseed rape

We studied the germination behaviour of the following types of seeds: weedy Brassica campestris, oilseed rape (Brassica napus), B. campestris (♀) × B. napus (♂), B. napus (♀) × B. campestris (♂) and, finally, seeds harvested on B. napus (♀) × B. campestris (♂) hybrids in open pollination with B. campestris and B. napus. The seeds were germinated in Petri dishes, using three different consecutive treatments and assaying the viability of non-germinated seeds with tetrazolium staining. B. campestris seeds varied in the treatment they required in order to germinate and many seeds were dormant, in contrast to B. napus seeds, which lacked dormancy. B. campestris (♀) × B. napus (♂) and B. napus (♀) × B. campestris (♂) seeds both resembled B. napus being non-dormant whereas seeds harvested on B. napus (♀) × B. campestris (♂) hybrids were more B. campestris-like in germination behaviour. We discuss implications for risk of transgene spread from oilseed rape to weedy B. campestris. This revised version was published online in July 2006 with corrections to the Cover Date.     

Soil Fertility Variability and Constraints on Village Scale Transects in Northern Burkina Faso

Soil variability has a profound impact on crop yields in low-input agriculture. The objectives of this study were to examine soil fertility variability and to identify potential soil constraints relating to farmers' conditions for agricultural production in the Sahelian zone of northern Burkina Faso. Surface soil (0-20 cm) and subsoil (20-100 cm) were sampled from 25 pedons on village-scale transects embracing three genetic soil units: two generations of sandy dune soils showing incipient development; a complex unit of clayey pediplain soils with duplex properties showing both alkaline and acid subsoil reaction; and hydromorphic, clayey valley bottom soils. Particle size distribution, pH, electrical conductivity, cation exchange capacity, exchangeable base cations and acidity, soluble base cations, organic carbon, nitrogen, total and available phosphorus, moisture constants, and bulk density were determined. Contents of organic carbon, total nitrogen, total and available phosphorus,and exchangeable potassium are low in all soils and are presumably major constraints.The sodium influenced clayey soils show high absolute variability; properties that vary are subsoil clay (5.8-38.5%), pH (5.8-10.0), cation exchange capacity (4.49-24.81 cmolc kg1), exchangeable sodium percentage (0-26), as well as electrical conductivity, structure, consistency, and available water holding capacity. The sandy soils are homogeneous and not as acid as elsewhere in the Sahel, presumably due to a dust influx. The soil fertility is generally low but highly variable, implying that constraints within production units may consist of multiple com binations of adverse chemical and physical properties, conceptually consistent with the low and variable millet yields. The variability also implies that soil character ization depends on the design of soil sampling, that average values are of doubtful use, that application of chemical fertilizers will produce very different results, and that there is no general scheme for correcting soil fertility. The application of lives tock manure will continue to be the best strategy for sustaining soil productivity.     

Quantification of Whole Blood Short-chain Fatty Acids by Gas Chromatographic Determination of Plasma 2-chloroethyl Derivatives and Correction for Dilution Space in Erythrocytes

A precise, accurate and stable method for quantification of short-chain fatty acids (SCFA) in ovine whole blood is presented and validated. The method is based on esterification of plasma SCFA by reaction with chloroethyl chloroformate in a water/acetonitrile/2-chloroethanol solution and gas chromatographic (GC) analysis of the derivatives. Whole blood concentrations of SCFA could be obtained by correcting plasma concentrations for a 45% dilution space of SCFA in the erythrocyte fraction of the blood. The recovery of SCFA in plasma and whole blood was 96-100% independent of the haematocrit value when compared with water standards. The method avoided carry-over from sample to sample, contrary to earlier published methods. The average intra-assay and inter-assay coefficient of variation for repeated measurement of SCFA content in plasma samples was 2.5% and 3.1%, respectively. The derivative was found to be suitable for a precise and accurate determination of the ¹³     

Protostar formation in the early universe

The nature of the first generation of stars in the universe remains largely unknown. Observations imply the existence of massive primordial stars early in the history of the universe, and the standard theory for the growth of cosmic structure predicts that structures grow hierarchically through gravitational instability. We have developed an ab initio computer simulation of the formation of primordial stars that follows the relevant atomic and molecular processes in a primordial gas in an expanding universe. The results show that primeval density fluctuations left over from the Big Bang can drive the formation of a tiny protostar with a mass 1% that of the Sun. The protostar is a seed for the subsequent formation of a massive primordial star.