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11.
The profile of catechol estrogen formation by pig conceptuses from d 14 to 20 of pregnancy (d 0 = 1st d of estrus) was studied using a direct product isolation assay for estrogen-2/4-hydroxylase (E-2/4-H). High performance liquid chromatography was coupled to detection of radioactive products in a flowing system to separate the 2- and 4-hydroxyestradiol (2- and 4-OH-E2) formed from estradiol. The major product was 2-OH-E2; its formation increased with increasing amounts of nicotinamide cofactors and exhibited a preference for NADPH. Maximum velocities were 909 and 154 pmol.mg protein-1.30 min-1, and apparent Michaelis constants were 7.14 and 5.12 microns, for 2- and 4-OH-E2 formation, respectively. Homogenates of d-13 conceptuses produced 1,151 +/- 142 pmol.mg protein-1.30 min-1 of 2-OH-E2. Estradiol-2-hydroxylase was affected (P less than .01) by day of pregnancy, and day effects from d 14 to 20 were described by a fourth-order equation (P less than .001). Conceptuses collected on d 14 produced 119 +/- 15.4 pmol of 2-OH-E2.mg protein-1.30 min-1. By d 15, E-2-activity had declined to 15.7 +/- 1.8 pmol.mg protein-1.30 min-1. Formation of 2-OH-E2 increased to 64.2 +/- 15.4 pmol.mg protein-1.30 min-1 on d 17, remained stable through d 19, and was 39.4 +/- 13.8 pmol.mg protein-1. 30 min-1 on d 20. Therefore, E-2/4-H activity appears to be regulated from d 14 to 20 of pregnancy in pigs; it may participate in the establishment of pregnancy. 相似文献
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Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis 总被引:4,自引:0,他引:4
Dey S Mohan CM Kumar TM Ramadass P Nainar AM Nachimuthu K 《Veterinary microbiology》2004,103(1-2):99-106
A recombinant antigen-based single serum dilution enzyme-linked immunosorbent assay (ELISA) was developed to measure the specific antibody activity in sera of dogs with leptospirosis. The recombinant antigen developed and used in the assay was specific for the pathogenic serovars of Leptospira. A linear relationship was found to exist between the predicted antibody titres at a single working dilution of 1:1000 and the corresponding observed serum titres as determined by the standard serial-dilution method. Regression analysis was used to determine a standard curve from which an equation can be derived that allows demonstration of the mentioned correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay was proved to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT). 相似文献
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Dey Daniel C. Gardiner Emile S. Schweitzer Callie J. Kabrick John M. Jacobs Douglass F. 《New Forests》2012,43(5-6):955-978
New Forests - Oaks (Quercus spp.) are one of the most important tree taxa in the northern hemisphere. Although they are dominant in mixed species forests and widely distributed, there are frequent... 相似文献
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Dey Daniel C. Knapp Benjamin O. Battaglia Mike A. Deal Robert L. Hart Justin L. O’Hara Kevin L. Schweitzer Callie J. Schuler Thomas M. 《New Forests》2019,50(1):11-40
New Forests - For millennia, natural disturbance regimes, including anthropogenic fire and hunting practices, have led to forest regeneration patterns that created a diversity of forest lands... 相似文献
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Jiangjiang Zhang Li Chang Jiangtao Yan Susmita Dey Defang Li Lining Zhao 《中国油料作物学报(英文)》2019,(2):110-117
In order to accelerate breeding progress of oil-used hemp, 8 quality traits related to lipid component were measured and analyzed in 55 representative hemp germplasms. Results showed that linolenic acid content had the highest variance coefficient of 51.67% as well as protein content had minimum variance coefficient of 4.07%. Correlation analysis showed that total oil content was significantly positively correlated to linolenic acid, while significantly negatively correlated with oleinic acid. Protein content was significantly positively correlated with linolenic acid but significantly negatively with stearic acid. Both total oil and protein content were closely correlated with other traits. The first 3 PCs from principal component analysis of 8 traits accounted for 83.53% contribution. All hemp germplasms were classified into 3 groups at the level of euclidian distance D=8.5 using cluster analysis. The above analysis and classification may provide a reliable theoretical basis and technological guidance for germplasms utilizing and accelerating breeding process. 相似文献
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Phytochemical composition and metabolic performance-enhancing activity of dietary berries traditionally used by Native North Americans 总被引:1,自引:0,他引:1
Burns Kraft TF Dey M Rogers RB Ribnicky DM Gipp DM Cefalu WT Raskin I Lila MA 《Journal of agricultural and food chemistry》2008,56(3):654-660
Four wild berry species, Amelanchier alnifolia, Viburnum trilobum, Prunus virginiana, and Shepherdia argentea, all integral to the traditional subsistence diet of Native American tribal communities, were evaluated to elucidate phytochemical composition and bioactive properties related to performance and human health. Biological activity was screened using a range of bioassays that assessed the potential for these little-known dietary berries to affect diabetic microvascular complications, hyperglycemia, pro-inflammatory gene expression, and metabolic syndrome symptoms. Nonpolar constituents from berries, including carotenoids, were potent inhibitors of aldose reductase (an enzyme involved in the etiology of diabetic microvascular complications), whereas the polar constituents, mainly phenolic acids, anthocyanins, and proanthocyanidins, were hypoglycemic agents and strong inhibitors of IL-1beta and COX-2 gene expression. Berry samples also showed the ability to modulate lipid metabolism and energy expenditure in a manner consistent with improving metabolic syndrome. The results demonstrate that these berries traditionally consumed by tribal cultures contain a rich array of phytochemicals that have the capacity to promote health and protect against chronic diseases, such as diabetes. 相似文献
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Panja Suraj Mondal Kongkong Kar Rup Kumar Dey Pradip Chandra Dey Narottam 《Journal of Crop Science and Biotechnology》2023,26(1):87-95
Journal of Crop Science and Biotechnology - Assamese Bora rice lines are valuable genetic resource for their socio-economic importance and traditional usage. Being lowland rice lines of upper... 相似文献