桑园施沼液好处多

随着各地新农村建设不断深入开展,农村旧村改造以及城镇旧城改造进一步扩大,农民人居环境得到了不断改善,从事畜禽生产的农户急剧减少,并逐渐向专业大户养殖发展。农户在种植土地上施用农家肥也越来越少,     

产甲壳素酶菌株的筛选与诱变

以胶体甲壳素作为惟一碳源,从自然界微生物中筛选并诱导到一株产甲壳素酶活性较高的菌株,经16S r DNA鉴定为Aeromonas sp.。并以该菌株作为出发菌株,经过一次紫外诱变,酶活是初筛菌株的1.48倍。再经过二次紫外诱变或者紫外-Li Cl复合诱变,酶活分别是初筛菌株的3.16和3.87倍,最高酶活达到3.48 U/m L。最后通过连续传代培养,确定了菌株HMX-16经过紫外和复合诱变后突变性状稳定性良好。     

国家经济安全审计实施路径研究

国家经济安全审计是指审计机关依赖有效的审计制度对被审单位财政、财务收支和经济活动的真实性、合法性和效益性进行审计的基础上，针对经济安全的关键领域，识别、评估、预警和报告危害经济安全的风险，确保财产权益安全完整的一种审计模式。该模式奠定了审计监督在国家经济安全体系中的基础性地位。创新审计模式及审计策略、变革国家审计体制、构建以审计监督为基础的新型国家经济安全体系是国家经济安全审计的三大实施路径。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

新媒体时代计算机专业就业指导课程教改探索

新媒体技术的广泛应用,对大学生尤其是计算机专业的大学生的思想行为和学习生活产生了深远的影响,在很大程度上拓展了教学空间,给计算机专业就业指导课程教学改革带来机遇。本文通过对计算机专业就业指导课程教学现状分析,把握新媒体技术带来的机遇,引入慕课教学和O2O模式教学,对新媒体时代计算机专业就业指导课程教学改革提出了可行的建议。     

公众参与城市生活垃圾管理的影响因素研究

基于公众视角,研究影响公众参与城市生活垃圾管理的因素及影响程度。通过设计调查问卷获得相关数据,利用主成分分析和回归分析方法对环境保护需求、法律制度保障等因素影响公众参与城市生活垃圾管理的程度进行实证研究。研究结果表明:相较于权利的追求、公众的利益追求、个人修养这些内部因素,环境保护需求、法律制度保障、环保组织推动这些外部因素对公众参与城市生活垃圾管理的推动作用要小。而社会监督力量和信息技术发展对公众参与城市生活垃圾管理的推动作用则表现不明显。研究可为城市市政相关部门制定政策提供指导和借鉴。     

辽宁省公众生态文明意识调查分析

通过对辽宁省不同人群进行问卷调查,结合层次分析法、专家打分法,获取评价指标权重,对辽宁省公众生态文明意识现状进行分析。结果表明,辽宁省公众生态文明意识整体较好,其中认同度践行度知晓度,表明辽宁省公民对生态文明的了解、认识与行为之间存在一定的差异。根据调查结果,提出相应建议,为辽宁省生态文明建设提供科学依据。     

压力蒸汽灭菌器的性能验证

压力蒸汽灭菌器是一种常见的消毒灭菌装置。通过开展灭菌效果验证可确认压力蒸汽灭菌器是否能在规定的时间、温度和压力条件下达到设备性能指标要求,试剂是否能被有效灭菌。     

Sublytic C5b-9 induces protective autophagy in cultured podocytes

AIM: In podocytes, autophagy occurs at a high basal level and dysregulated autophagy is associa-ted with a variety of podocytopathies. This paper is to investigate the role of autophagy in sublytic C5b-9-induced podocyte injury. METHODS: Sublytic complement C5b-9 stimulation was used as an in vitro model. Autophagosomes were confirmed using monodansylcadaverine (MDC) staining. Immunoblotting was used to measure the change of autophagy-related markers. Cellular morphological changes were observed by Wright-Giemsa staining. Immunofluorescence staining and confocal microscopy were used to detect the expression and distribution of nephrin. The cell viability was assessed by methylthiazol tetrazolium (MTT) assay. The cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/PI staining. RESULTS: For ensuring sublytic complement injury, the maximal amounts of anti-podocyte antiserum and 160×-diluted normal human serum were used without inducing cell lysis (defined as >5% LDH release). Sublytic C5b-9 promoted autophagy of podocytes in vitro. The proautophagic effect of sublytic C5b-9 manifested in the form of accumulated MDC-labeled vesicles and enhanced the expression of LC3-Ⅱ. Autophagy inhibitor 3-methyladenosine (3-MA) promoted sublytic C5b-9-induced podocyte morphological abnormalities. Compared with the sublytic C5b-9-injured podocytes, 3-MA exposure further decreased the expression of nephrin. 3-MA enhanced sublytic C5b-9-induced podocyte apoptosis. CONCLUSION: Sublytic C5b-9 attack induces autophagy, which may play a protective role against complement-mediated podocyte injury.