TTC法快速测定白鲜种子生活力

采用2,3,5-氯化三苯基四氮唑(TTC)染色法测定白鲜种子生活力,利用正交试验设计,研究不同的浸种温度、浸种时间、TTC溶液浓度、染色温度和染色时间对白鲜种子染色效果的影响。结果表明,TTC法能够快速测定白鲜种子的生活力,其染色最佳条件为30℃浸种6 h,35℃条件下0.3%TTC溶液避光染色24 h。     

食用花生油品质状况检测

食用花生油是日常生活中量大面广的消费食品,为评估食用花生油品质,对3项主要指标酸价、过氧化值及黄曲霉毒素B1(AFB1)进行了检测。结果表明,440批次样品的不合格率为32%,主要不合格项目为AFB1,不合格样品主要来源为小油坊。食用花生油仍存在食品安全隐患,为安全起见,建议消费者最好购买品牌产品。     

猪TOLL样受体7全基因的扩增、克隆及生物信息学分析

根据猪Toll样受体7基因(TLR7)序列设计、合成1对引物,直接从三元猪外周血淋巴细胞中提取总RNA,应用RT-PCR技术扩增三元猪TLR7全基因,并进行克隆和测序.测序结果表明,获得了三元猪TLR7全基因序列,其大小为3 160 bp,包含一个完整阅读框(3 153 bp),编码1 051个氨基酸残基,有15个潜在的N-糖基化位点和4个潜在的磷酸化位点,信号肽切割部位位于第29～30位氨基酸之间,跨膜基因序列为第844～866位.TLR7基因存在种属的差异性,亲缘关系越近,同源性越高.三元猪TLR7基因的成功克隆为进一步研究猪TLR7基因表达、生物学功能、揭示ssRNA病毒入侵宿主的致病机制以及猪抗病毒免疫及育种的相关研究奠定了基础.     

不同诱导因子对盾叶薯蓣试管苗生根的影响

为获得大量盾叶薯蓣试管苗以进行规模化生产,以盾叶薯蓣顶芽为外植体,研究了不同基本培养基(1/2 MS和MS)和生长素(NAA和IBA)对试管苗生根的影响,得到较有利于生根的基本培养基和生长素种类及浓度;在含有生长素的培养基中添加不同浓度的硝酸铈[Ce(NO3)3]或活性炭,综合生根率、根数、根长、株高、叶片数等指标,筛选最佳生根培养基配方。结果表明:1/2 MS、IBA和Ce(NO3)3利于盾叶薯蓣试管苗的生根,NAA和活性炭不利于盾叶薯蓣试管苗的生根。盾叶薯蓣试管苗最佳生根培养基为1/2 MS+IBA 0.3 mg/L+Ce(NO3)35 mg/L,生根率达98.75%,根16.1条,长4.17 cm,试管苗高3.86 cm,有叶片6.44个。可用于盾叶薯蓣试管苗的推广种植。     

甘蓝型油菜MAPK7基因家族及其启动子的克隆与表达分析

从甘蓝型油菜中克隆了MAPK7基因家族3个成员BnMAPK7-1、BnMAPK7-2和BnMAPK7-3的全长cDNA序列,它们最长标准mRNA分别为1593、1434和1547 bp。系统进化分析表明,甘蓝型油菜MAPK7基因全部来自白菜,而且BnMAPK7-1/-2的是由白菜Bra03723祖先基因加倍产生的,而BnMAPK7-3则由白菜中Bra037234的祖先基因进化形成的。同时,克隆获得BnMAPK7-1/-2的启动子序列,该启动子里含有多个与光诱导相关的元件、激素响应元件和逆境胁迫响应元件。实时荧光定量PCR (qRT-PCR)显示甘蓝型油菜MAPK7基因家族在所有的组织器官中均表达,并发现该基因家族的表达受植物激素(MeJA、ABA、SA)、信号分子(H₂O₂)、逆境(高温)以及伤害(损伤和核盘菌)的诱导,初步证明甘蓝型油菜MAPK7基因在植物逆境胁迫应答中起一定作用。实现了甘蓝型油菜MAPK7-1/-3基因的原核表达,并证明它们编码的蛋白主要以包涵体形式存在,为后续的研究提供了基础。     

均质压力对豆乳贮存过程中物性变化的影响

以豆乳为原料,通过不同均质压力处理后,将样品存放在常温(25℃)环境下,持续观测30 d,研究不同均质压力下贮存过程中豆乳的黏度、色差、丙二醛含量和蛋白稳定性的变化。发现在15～40 MPa的均质压力下,豆乳黏度无明显差别;贮存过程中,色差△E值在第20天时为8.51,感官评价消费者中不可接受,黏度和稳定性有极大差异。     

Effect of the Silicic Acid Polymerization Degree on the Coagulating Property of Polyalumium Silicate Chloride

Polyaluminum silicate is a new type of inorganic macromolecular flocculants, which is developed as a complex product in recent years on the base of polyalumium flocculant and polysilicic acid flocculant. Sodium silicate is diluted to the concentration of 3.0% SiO 2 with distilled water. The solution was adjusted to pH 4.5 with sulphate acid and kept at quiescency for different time to get polysilicic acid with different polymerization degrees. Using polysilicic acid prepared and aluminium chloride as materials, a series of polyaluminium silicate chlorides(PASC) with different basicities(B), Al/Si molar ratios and polymerization degree of silicic acid are prepared by copolymerization technique. The Al-Ferron timed complex colorimetric method is applied to characterize species distribution of PASC and to study its influence factors. The interaction between hydrolyzed aluminum species and polysilicic acid with different polymerization degrees in PASC is discussed.The flocculant prepared is applied to the treatment of commercial concrete wastewater. The turbidity removal of commercial concrete wastewater is up to 97% when a PASC, prepared with polysilicic acid of 145 min polymerization degree.     

Determination of Moisture Rate of Porous Saline Materials

The high humidity makes the indoor air quality sultry. The porous saline materials can function as efficient humidity conditioner. On the basis of analyzing mass exchange process between porous saline materials and air, the computational method of the moisture exchange rate on the porous saline materials surface is established. A number of methods for enhancing the humidity conditioning of porous saline materials are suggested.     

农杆菌介导法获得小麦转基因植株的研究

从gus基因的瞬时表达入手, 利用不同种类的农杆菌菌株感染小麦不同基因型和同一基因型的不同外植体, 研究了农杆菌菌系、小麦基因型和外植体对转化效率的影响. 从中优选出了对小麦愈伤组织感染力比较强的农杆菌菌系AGL0和MOG101, 以及高敏感受体基因型Alondra和扬麦158等. 实验结果还表明, 预培养10～15天的幼胚愈伤组织具有     

Model About Aggregation and Spreading Effect Brought by the Lightrail

Many cities are planning to build up the lightrail in order to solve their traffic jam.We try to find a function to describe the going-out cost and analyse the character of this function.The conclusion can be reached that lighrail can make aggregation and spreading effect on the economic activity in the district along the lightrail transit line because the change of cost brought by the lightrail traffic system lead to redistribution of the product region. Synthetically considering factors which can impact this aggregation and spreading, via applying the method of AHP, we set up an evaluation system which can be used to measure the economic effect brought by the lighrail.