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41.
Expression of Genes Associated with Apoptosis in the Porcine Corpus Luteum During the Oestrous Cycle
The corpus luteum (CL) of the pig lacks luteolytic sensitivity (LS) to prostaglandin (PG) F‐2α until after day 12 of the oestrous cycle, but the mechanisms underlying this phenomenon are poorly understood. As luteolysis involves apoptosis, we hypothesized that critical apoptotic proteins may be deficient in CLs that lack LS. The specific aim of these studies was to examine mRNA expression and protein levels of apoptosis genes/proteins (BAX/Bax, BCLX/Bcl‐x, CASP3/Caspase‐3, CASP8/Caspase‐8, NFΚB1/NFκB, TP53/p53) in porcine CLs collected at different stages of the oestrous cycle. CLs were collected surgically, mRNA and protein extracted, and expression/levels analyzed by semi‐quantitative (SQ) PCR and Western blots, respectively. At the mRNA expression level, only BAX (maximal on day 4) and TP53 (maximal on day 7) showed significant variations during the oestrous cycle. At the protein level, only Bcl‐x and Caspase‐3 showed significant changes during the cycle; Bcl‐x decreased on day 13 and Caspase‐3 increased on day 13. It is concluded that apoptosis‐associated proteins (i.e. Bcl‐x and Caspase 3) may play a critical role in luteolytic sensitivity in the pig. 相似文献
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Ivermectin toxicosis in a neonatal foal 总被引:1,自引:0,他引:1
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Bovine citrullinaemia traced to the sire of Linmack Kriss King 总被引:1,自引:0,他引:1
PJ HEALY JA DENNIS LM CAMILLERI JL ROBINSON AL STELL RD SHANKS 《Australian veterinary journal》1991,68(4):155-155
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The wild South American camelids developed a strategy of seasonal reproduction during spring and summer with singleton birth. The photoperiod is one of the factors that may modulate this seasonality where light would be translated into a hormonal signal. This study evaluated the influence of changes in daily light intensity on melatonin concentration in captive guanacos under a long‐day photoperiod (16 hr light/8 hr dark; 33 ‘28′S). Mean melatonin concentration was 28.3 ± 20.3 pg/ml, with a maximum of 52.14 ± 17.19 pg/ml at 23:30 and minimum of 14.29 ± 6.64 pg/ml at 08:30 (p < .001). There was a negative association between light intensity and melatonin concentration (r = ?0.57; p < .001). The results indicate that guanacos respond to variation in daily environmental light with a hormonal response and point to a circannual rhythm as a function of the photoperiod. 相似文献
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The phase velocities of Rayleigh waves increase more rapidly with distance from the East Pacific Rise (EPR) axis than is predicted by models of conductive cooling of the lithosphere. Low velocities near the axis are probably caused by partial melt at depths of 20 to 70 kilometers in a zone several hundred kilometers wide. The lowest velocities are offset to the west of the EPR. Wave propagation is anisotropic; the fast direction is approximately perpendicular to the ridge, parallel to the spreading direction. Anisotropy increases from a minimum near the axis to 3 percent or more on the flanks. 相似文献