沈阳地区高氟地下水形成机理研究

根据沈阳地区多年水文地质和气象资料,初步探明了该地区高氟地下水的主要分布区域,并从水文地质等因素方面分析了该地区高氟地下水的形成机理。     

3种喷雾助剂对呋虫胺防治苹果黄蚜的增效作用

采用室内毒力测定、助剂润湿铺展性能和田间效果评价研究了3种功能助剂NF-100、GY-T1602和迈道对呋虫胺防治苹果黄蚜的减施增效作用。结果表明,NF-100、GY-T1602和迈道3种助剂均未能提高呋虫胺对苹果黄蚜的毒力;20%呋虫胺SG 1 110～5 000倍液中分别添加0.1%NF-100、0.5%GY-T1602和0.3%迈道,表面张力降低6.97～37.44 mN/m、静态接触角降低10.58°～48.67°、黏附张力增加2.32～21.71 mN/m; 20%呋虫胺SG(有效成分用量32 mg/kg)减量处理添加3种助剂药后3～15 d对苹果黄蚜田间防效达90.83%～99.33%,其中添加NF-100和GY-T1602药后15 d防效均显著高于添加迈道处理及20%呋虫胺SG(40 mg/kg)未减量处理。因此,喷雾助剂NF-100和GY-T1602可显著改善呋虫胺药液的润湿铺展性能,减少呋虫胺有效成分用量20%,对苹果黄蚜仍有较高防效、且持效期延长。     

陕北丘陵沟壑区畜牧业发展问题研究

陕北丘陵沟壑区是陕北水土流失最严重的区域,也是国家黄土高原综合治理的主要区域。由于生态建设的需要,以放牧为主的畜牧业面临挑战。本文在对陕北丘陵沟壑区畜牧业发展现状和存在问题调查研究的基础上,分析了该区粮食生产对畜牧业的影响,提出了陕北丘陵沟壑区不同种类家畜发展的方向及畜牧业发展总体思路：陕北丘陵沟壑区应以舍饲养羊为主,全面发展各种适宜舍饲的畜禽品种;以生态建设和经济发展为目标,促进陕北畜牧业可持续发展。     

不同日粮组成对断奶初期奶公犊瘤胃内环境指标的影响

选用3头三月龄左右装有瘤胃瘘管的荷斯坦奶公犊,按3×3完全拉丁方试验设计,喂给三种日粮.日粮组成按精粗比分别表示为7030;6040;5050,研究不同日粮组成对断奶初期奶公犊瘤胃内环境指标包括pH值、氨氮(NH3-N)浓度、纤毛虫数量及微生物蛋白的影响.试验结果表明精粗比为7030日粮条件下奶公犊瘤胃内pH值显著低于其它组(P＜0.01),7030日粮组瘤胃内NH3-N浓度显著高于精粗比为6040和5050日粮(P＜0.01).不同日粮组成下精粗比为6040和5050日粮的瘤胃纤毛虫数量和微生物蛋白显著高于高精料组7030日粮(P＜0.01).     

海水泵用材料的选择比较

该文通过对铸铁，铜合金及0Cr17Ni12Mo2(日本JIS标准SUS316）不锈钢等3种材料在海水泵中的使用情况进行分析比较，并在为香港地区输送海水而生产制造海水泵的长期实践中证明，使用SUS316不锈钢且严格 控制其铸造工艺和其它热处理工艺，则SUS316具有较强的耐海水腐蚀能力及较高的性能价格比，因此，该材料应用在耐海水泵中具有广阔的发展前景。     

植物根围促生细菌菌剂增强玉米干旱耐受性研究

选用植物根围促生细菌(PGPR)菌剂BBS处理玉米幼苗,研究干旱胁迫下玉米幼苗叶片相关生理特性及基因表达差异。结果表明,干旱胁迫15 d后,BBS处理组玉米幼苗的萎蔫程度显著低于对照组;处理组植株叶片MDA含量仅为对照组的74.35%,叶片脯氨酸和可溶性糖含量分别是对照组的3.39倍和1.07倍;处理组植株叶片ZmP5CS1的表达水平及SOD活性均显著高于对照组;BBS处理组植株叶片在干旱胁迫过程中一直保持较低的H_2O_2含量。研究得出,BBS处理能够有效减缓干旱胁迫对玉米幼苗造成的伤害,进而加速正常浇水后的恢复。处理组植株叶片NCED1和ZmDREB2.7的高水平表达也表明干旱胁迫下玉米幼苗与BBS的互作过程可能有依赖ABA信号途径及不依赖ABA信号途径的共同参与。     

瞬时表达比较2种不同的RNAi载体的干涉效果

 RNAi with natural defence mechanism of homologous RNA degradation is widely used in research of antiviral plant. It is important to construct a highly efficent RNAi vector for transgenic plants of virus resis-tance. In this study, part fragments of coat protein gene of Potato virus Y (PVY) (451-750 bp) were inserted into the two expression vectors. Vector pROKY300 without intron and pHelY300 with PDK and CAT introns on the hpRNA stem were constructed. The silence efficiency of virus resistance of the two vectors was investigated as 88% (22/25)for pROKY300 and 92% (23/25) for pHelY300 through transient expression mediated by agroinfiltration. The results showed that both vectors were highly antiviral and elucidated the validity of RNAi-medicates resistance to virus.     

Over-expression of P21 attenuates cisplatin-induced HK-2 cells injury

AIM:To investigate the effect of P21 on cisplatin-induced renal tubular epithelial cells injury.METHODS:The expression of P21 at mRNA and protein levels in cisplatin treated human renal tubular epithelial cells (HK-2) cells was determined by RT-qPCR and Western blot. Over-expression of P21in the HK-2 cells was induced by the transfection of pcDNA3-P21. The cell viability and cell apoptosis were detected by CCK-8 assay and flow cytometry, respectively. Furthermore, the protein expression of kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), caspase-3, glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP), and phosphorylation level of eucaryotic translation initiation factor 2α (eIF2α) and protein kinase R-like endoplasmic reticulum kinase (PERK) were detected by Western blot.RESULTS:Cisplatin increased the mRNA and protein levels of P21 in a time-and concentration-dependent manner in the HK-2 cells. Over-expression of P21 inhibited cisplatin-induced cell apoptosis, and down-regulated the expression of KIM-1 and NGAL. Furthermore, Over-expression of P21 decreased the protein levels of GRP78, p-PERK, p-eIF2α, CHOP and cleaved caspase-3.CONCLUSION:Over-expression of P21 attenuates cisplatin-induced HK-2 cells injury, and the mechanism may be related to the modulation of endoplasmic reticulum stress pathway and inhibition of cell apoptosis.     

塞北兔品系选育初探

根据塞北兔品种特征及其毛色分离现象,组建0世代品系基础群,采用闭锁群体继代选育法进行品系选育。经过5个世代连续选育,塞北兔各品系毛色纯合度逐渐提高,同时各品系的生长发育性状和繁殖性状无显著变化,使原塞北兔种兔群逐渐形成了遗传稳定的野灰色、白色、红黄色3种被毛颜色的群体。     

抗缩果病和抗裂果枣树新品种‘曙光 3 号’

 ‘曙光3号’是从河北省太行山区‘婆枣’资源中选育出来的抗枣缩果病、抗裂果的优良枣树新品种,果实圆形,平均单果质量19.3 g,鲜枣可溶性固形物含量23.1%,酸甜味浓。丰产稳产,抗缩果、抗裂果,适宜干制,适合河北太行山枣区栽培。在河北枣区10月上旬成熟。