Effect of diet with high fats and cholesterol on PPARα gene expression and body cholesterol level in mice

AIM:To investigate the effects of dietary fats and cholesterol on liver PPARα gene expression and body cholesterol (Chol) level in C57BL/6J mice. METHODS:The animals (n=75) were randomly divided into five groups and respectively received formula mash for 6 weeks. RESULTS:As compared to Chol diet, Chol+PUFA diet produced significantly higher liver cholesterol (P<0.01), serum total cholesterol (TC), focusing on HDL-C. While Chol+MUFA diet resulted in unchanged serum TC and lower liver cholesterol (P<0.01). Chol+SFA diet rsulted in higher liver cholesterol (P<0.01) and serum TC, focusing on LDL-C. Furthermore, Chol+PUFA diet increased the mRNA and protein content of PPARα (P<0.01) in liver, while Chol+MUFA and Chol+SFA diets decreased the mRNA content of PPARα significantly (P<0.01). CONCLUSIONS:These results indicated that addition of fats containing PUFA to diet high in cholesterol increased PPARα mRNA and protein expression, addition of fats containing MUFA or SFA to diet high in cholesterol decreased PPARα mRNA expression. The change of PPARα gene expression may further affect body cholesterol level.     

Role of neutrophil elastase and myeloperoxidase in asthma of rats

AIM:To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS:Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated andpurified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS:(1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P<0.01). Activities of MPO were significantly increased in bronchoalveolar lavage fluid (BALF) and lung tissue in asthma group compared with those in control group (P<0.05, P<0.01). (2) Levels of NE were significantly increased in BALF and PMN in asthma group compared with control group (P<0.01). (3) Number of PMN were significantly higher in BALF, bronchus and lung tissue in asthma group than those in control group (P<0.01). CONCLUSION:Levels of PMN counting and expression of MPO and NEare significantly increased in experimental asthma rats. PMN may be involved in inflammation in asthma via increasing the levels of NE and MPO.     

Effect of mitochondrial ATP sensitive potassium channels opening on translocation of protein kinase C epsilon in cultured adult rat ventricular myocytes

AIM: To investigate the effects of mitochondrial ATP sensitive potassium (MitoKATP) channel opening on translocation of protein kinase C epsilon (PKCε) and the relationship between the translocation of PKCε and the production of reactive oxygen species. METHODS: The expression of PKCε in cultured adult rat ventricular myocytes was investigated with immunofluorescence and Western blotting techniques. RESULTS: （1） Diazoxide, a selective MitoKATP channel opener, caused a significant translocation to myofibrillar-like structures in cultured adult rat ventricular myocytes. (2) The N-2-mercaptopropionylglycine (MPG), a free radical scavenger, partly inhibited the translocation of PKCε caused by diazoxide. (3) Chelerythrine, a selective protein kinase C (PKC) inhibitor, completely blocked the translocation of PKCε caused by diazoxide. CONCLUSION: Opening of MitoKATP channel might activate PKCε and make it translocation to myofibrillar-like structures. PKCε activation occurs downstream of MitoKATP channel, and might be caused by production of reactive oxygen species after opening of MitoKATP channel.     

谈谈兔的A型魏氏梭菌感染

近年来,我国不少养兔地区发生一种以急性下痢为主要特征的兔病,有的养兔场发率病达90%,死亡率常高达100%,而且用各种抗菌药物治疗无效,造成很大的经济损失.我国早在1984年就已确诊兔急性下痢的主要病原是A型魏氏梭茵,并研制出了有效的菌苗和高免血清,但许多基层兽医工作者对此尚无深入的了解和认识.现将本病的流行特点、症状、防治等介绍如下,供参考.     

秃杉种质资源迁地保存育苗技术研究

总结研究了13a来在地处亚热带在江苏省宁镇丘陵地区引种原产云贵高原、鄂西山地的珍稀树种秃杉获得在播种育苗与无性繁殖技术成果。该项 成果认为采用氧化剂消毒浸种侵芽,适时防治幼苗猝倒病,适度遮荫保湿,当年冬季防寒保苗等组装技术,可使每公斤种子产秃杉合格苗2．20万－5．60万株,1年生苗安全越冬;采用砻糠灰作基质,用KMnO4液浸条,在秋冬季扦插,生根率达41．3％－54．4％;用电子叶间隙喷雾,以珍珠岩作基质,或在日光温室内用塑料薄膜封闭保温,用KMnO4液浸条等技术,可使插条生根率达72．6％和76．1％。     

我国秦川牛等四品种黄牛屠宰试验报告

本屠宰试验所用良种黄牛品种系鲁西牛(25头)、秦川牛(29头)、晋南牛(28头)、南阳牛(26头)4个品种共108头。屠宰前平均活重(未停水停食处理)为554.15±67.6kg;屠宰率达63.30±1.84％;净肉率为53.65±2.34％;可供4～5星级饭店的高档牛肉重约占活重的5.2％,其产值却可占总产值的46％以上。经北京市4～5星级饭店品尝、试用、使用,各用户一致认为牛肉品质已达到进口的同类产品的最优等级,完全可以取代进口牛肉。     

防病生物有机肥在设施蔬菜生产中的应用

防病生物有机肥应用于设施蔬菜生产,将有效减少化学农药在设施生产中的使用,提高蔬菜品质,符合无公害蔬菜生产的需求。     

浅析网络环境下农业科技图书馆的联合服务

在网络环境下,开展农业科技图书馆的联合服务很有必要,但存在管理模式落后,材料不规范、技术滞后、人员素质低等问题.对策是一重要新观念,变被动服务为主动服务;二要建设信息站点,提高Web的访问率,提供反馈窗口.注重资源库建设,提高工作效率;三要正确处理好联合开发利用与保护知识产权的关系.     

苏打盐碱土种植水稻试验总结

通过试验研究,检验了磷石膏和硫酸铝这两种改良剂的实际效果,证明磷石膏能够有效降低苏打盐碱土的pH值,改善土壤的结构,增加土壤通透性,加快土壤熟化进程,减轻苏打盐碱土对水稻的危害,显著提高水稻产量.总结出一般中轻度苏打盐碱土种植水稻的关键技术,为利用中轻度苏打盐碱土提供了宝贵经验.