Characteristics of 18F-FDG and 18F-FDOPA PET in an 8-year-old neutered male Yorkshire Terrier dog with glioma: long-term chemotherapy using hydroxyurea plus imatinib with prednisolone and immunoreactivity for PDGFR-β and LAT1

An 8-year-old neutered male Yorkshire Terrier dog presented with head pressing, vestibular ataxia, neck tenderness, and no oculocephalic reflex. A demarcated lesion in the pons was identified on MRI. The patient was tentatively diagnosed with a glioma and was treated with hydroxyurea plus imatinib and prednisolone. After 30 days of therapeutic treatment, the patient showed a clear improvement in neurological signs, which lasted for 1117 days. On day 569 after the initiation of treatment, ¹⁸F-fluorodeoxyglucose (FDG)-positron emission tomography (PET) was performed with no significant findings on visual analysis. The average and maximal standardized uptake values (SUVs) were 1.92 and 2.29, respectively. The tumor-to-normal-tissue (T/N) ratio was 0.97. The first evidence of clinical deterioration was noticed on day 1147. On day 1155, 3,4-dihydroxy-6-[¹⁸F]-fluoro-l-phenylalanine (¹⁸F-FDOPA)-PET was performed. High uptake of ¹⁸F-FDOPA was observed in the intracranial lesion. The mean and maximal SUVs of the tumor were 1.59 and 2.29, respectively. The T/N ratio was 2.22. The patient was euthanized on day 1155 and histopathologic evaluations confirmed glioma (astrocytoma). This case shows that chemotherapy with hydroxyurea plus imatinib may be considered in the treatment of canine glioma. Furthermore, this is the first case describing the application of ¹⁸F-FDG and ¹⁸F-FDOPA in a dog with glioma.     

Starch digestion rates in multiple samples of commonly used feed grains in diets for broiler chickens

In this study the starch digestion rates in broiler chickens from 18 samples of 5 commonly used feed grains (sorghum, wheat, maize, barley, triticale) were determined. The methodology to determine starch digestion rates in poultry is detailed herein. Starch digestion rates were not significantly different (P = 0.128) across the 18 feed grains, which reflects the wide variations that were observed within a given feedstuff. Nevertheless, starch digestion rates in broiler chickens offered wheat-based diets were significantly more rapid by 56.0% (0.117 versus 0.075 min⁻¹; P = 0.012) than their sorghum-based counterparts on the basis of a pair-wise comparison. In descending order, the following starch digestion rates were observed: wheat (0.117 min⁻¹), barley (0.104 min⁻¹), triticale (0.093 min⁻¹), maize (0.086 min⁻¹), sorghum (0.075 min⁻¹). The implications of these findings are discussed as they almost certainly have implications for poultry nutrition and the development of reduced crude protein diets for broiler chickens.     

成长型饲料企业人力资源面临的问题及解决策略研究

企业的发展通常要遵循企业的生命周期理论,从企业最初创立进入生存期,到企业规模进一步增大进入成长期,再到企业成熟期和衰退期,每个生命周期阶段的生产经营和管理策略都有所不同.当企业进入成长期,其发展潜力进一步释放,生产销售数据呈现快速增长的趋势,企业的管理形式也逐渐趋向成熟.对于成长型的企业而言,人力资源管理水平和现状关乎...     

Inactivation of the Wnt/β-catenin signaling contributes to the epithelial barrier dysfunction induced by sodium oxalate in canine renal epithelial cells

High oxalate consumption has been recognized as a risk factor for renal calcium oxalate stones in companion animals (dogs and cats). However, the cellular signaling involved in oxalate-induced dysfunction in renal tubular epithelial cells remains not fully elucidated. In this study, Mardin–Darby canine kidney (MDCK) cells, an epithelial cell line derived from canine kidney tubule, were tested for cell proliferation activity and barrier function after being exposed to sodium oxalate (NaOx). Further, the involvement of Wnt/β-catenin in NaOx-induced renal epithelial barrier dysfunction was evaluated. MDCK cells treated with NaOx exhibited reduction in cell proliferation and migration. Besides, NaOx exposure led to a decrease in transepithelial electrical resistance and an increase in paracellular permeability. The deleterious effects of NaOx on epithelial barrier function were related to the suppressed abundance of tight junction proteins including zonula occludens, occludin, and claudin-1. Of note, protein levels of β-catenin and phosphorylated (p)-β-catenin (Ser552) in MDCK cells were repressed by NaOx, indicating inhibitory effects on Wnt/β-catenin signaling. An inhibition of glycogen synthase kinase-3β (GSK-3β) by SB216763 enhanced the abundance of β-catenin and p-β-catenin (Ser552), and protected against epithelial barrier dysfunction in NaOx-treated MDCK cells. The results revealed a critical role of Wnt/β-catenin signaling in the epithelial barrier function of MDCK cells. Activation of Wnt/β-catenin signaling might be a potential therapeutic target for the treatment of oxalate-linked renal stones.     

密码子优化的鸡白细胞介素-17在昆虫细胞中的表达研究

为了获得具有良好生物学活性且具有较高表达量的鸡白细胞介素-17(ChIL-17),本研究在前期研究工作基础上,将ChIL-17的编码基因按照真核细胞(昆虫细胞)偏爱的密码子进行优化改造,经全基因合成后插入到转座载体pFastBacTM Ⅰ中,构建重组转移质粒pfast-mod.ChIL-17并转化DH10Bac感受态细胞.通过位点特异性转座将mod.ChIL-17基因整合到穿梭质粒Bacmid中,获得重组穿梭质粒Bacmid-mod.ChIL-17.应用脂质体将重组穿梭质粒转染Sf9昆虫细胞,获得重组杆状病毒rBac-mod.ChIL-17.重组病毒传代扩增感染Sf9细胞,通过间接免疫荧光检测目的蛋白的表达.结果表明,经过优化的ChIL-17在杆状病毒系统中获得表达.     

养鱼池塘增氧机械产氧量比较

为比较分析不同类型机械增氧效果,选择扬水水车式、微管鼓风式、涡轮叶片式和扬水喷水式4种增氧机械,采用滴定法测定开机前后养鱼池塘溶氧量,用养殖种类、增氧时间校正,同一时间内,单位功率的产氧量分别是3953.42、735.66、616.58、177.55/(g/kw.h),扬水水车式显著高于其他增氧方式产氧量。     

肉牛冻精细管无伤害制作技术

本文对日本和牛冻精细管生产过程中的种公牛饲养管理、采精管理、稀释液的配方及其配制过程,稀释过程,冷冻过程进行了详细描述。这项技术使细管冻精的平均活力由的0.3提高到0.4以上,大幅提高了冻精生产质量。     

复方中草药添加剂对稻鸭共作模式下成都麻鸭生理效应的影响研究

为研究复方中草药添加剂对稻鸭共作模式下成都麻鸭生长性能、血清生化指标、抗氧化指标及免疫指标的影响,试验选取体重相近、体况健康的15日龄成都麻鸭240只,随机分为4组,每组5个重复,每个重复12只。4组为对照组饲喂基础饲粮;试验1、2、3组在基础饲粮中按照质量比分别添加0.5%(低剂量组)、1%(中剂量组)、2%(高剂量组)的复方中草药,试验期90 d。结果显示:(1)试验1、2、3组的平均日增重分别较对照组提高2.19%、4.34%、4.98%(P<0.05);试验2、3组的料重比分别较对照组降低3.37%、3.63%(P<0.05);(2)试验2组和3组的总蛋白(TP)分别较对照组提高4.20%、6.08%(P<0.05);试验2组和3组的尿素氮(BUN)分别较对照组降低12.72%、16.36%(P<0.05);试验2组和3组的谷草转氨酶(ALT)分别较对照组降低15.84%、19.22%(P<0.05);试验3组的谷丙转氨酶(AST)较对照组降低19.34%(P<0.05);(3)试验2组和3组超氧化物歧化酶(SOD)分别较对照组提高15.80%、20.00%(P<0.05);试验3组的总抗氧化能力(T-AOC)较对照组提高22.37%(P<0.05);(4)三个试验组的IgA、IgM、IgG、IFN-γ、IL-2显著高于对照组(P<0.05),试验2组的肿瘤坏死因子-α(TNF-α)较对照组提高10.06%(P<0.05),试验2组和3的组溶菌酶(LZM)分别较对照组提高6.79%、5.66%(P<0.05)。综上,中草药添加剂能有效提高稻鸭共作模式下成都麻鸭的生长性能、血清生化指标、抗氧化指标及免疫指标,本试验条件下,最佳饲喂剂量为1%。     

四种动物凝血三项值初探

为了探索正常生理状态下4种动物的凝血3项值,分别用凝血酶原时间测定试剂盒、自制兔脑粉浸出液、自制鸡脑粉浸出液测定了鸡、鲤鱼、犬和羊4种动物的血浆凝血酶原时间(PT),用试剂盒测定了4种动物的凝血酶时间(TT)和活化部分凝血酶原时间(APTT).结果表明:3种方法测定的4种动物的PT值在相同动物间无显著差异(P>0.05),说明有颌类脊椎动物体内的组织因子在启动血液凝固的作用上具有进化的保守性;4种动物的凝血3项值分别为鸡PT 43.42 s ,TT 16.85 s ,APTT 91.33 s;鲤鱼PT 73.39 s ,TT 20.75 s ,APTT 12.80 s;犬PT 10.70 s ,TT 13.32 s ,APTT 25.89 s;羊PT 23.29 s ,TT 8.04 s ,APTT 25.92 s.     

O型口蹄疫病毒P1-2A3C基因在家蚕杆状病毒表达系统中的表达

口蹄疫是一种严重危害畜牧业生产的烈性传染病。为了促进O型口蹄疫病毒(FMDV)基因工程活载体疫苗的研制,选取O型FMDV编码序列中的衣壳蛋白前体P1-2A基因和蛋白酶3C基因,插入家蚕杆状病毒转移载体pVL1393中,构建重组载体pVL-P1-2A3C,并与线性化病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞,获得重组病毒Bm-P1-2A3C。将重组病毒感染家蚕5龄幼虫,以双抗体夹心ELISA法和间接血凝方法检测血淋巴中的表达产物:目的蛋白在感染病毒后120 h的蚕血淋巴中表达量最高,抗原表达呈阳性的最大稀释倍数为1∶128。结果显示O型FMDV的P1-2A3C基因已在家蚕体内获得表达。