汽车前照灯智能控制系统的设计

基于汽车驾驶的安全性,结合传感器技术和微控制技术,对汽车前照灯设计了一套智能控制系统。该系统能在不同的情况下,对前照灯的照射角度和亮度进行自动实时调整和自动选择适宜的灯光模式,实现汽车前照灯的智能化控制,提高了汽车驾驶的安全性、舒适性以及智能感觉。     

东西汊湖水资源合理配置研究

以应城市东西汊湖为例,建立了以经济、生态和社会效益为目标的区域可持续发展水资源优化配置模型,对该区域的水资源合理配置进行了初步的研究。研究结果表明,模型能够准确、合理地反应该区域可持续发展的各项指标。从而可为东西汊湖流域的水资源配置提供技术支持。     

采用施奈德重合器实现馈线自动化的实践研究

针对基于传统重合器的馈线自动化原理及存在问题,提出基于施奈德重合器的馈线自动化方案。以大连庄河炮台变电所石山线-龙王庙变电所水泥线为例,分析施奈德重合器的馈线自动化工作原理,指出该馈线自动化系统在实践中的优点和不足。     

玉米机械精量播种技术在彰武地区的应用研究

介绍几年来彰武县玉米精量播种技术的推广应用情况,通过与传统播种方式田间对比试验,证明采用精量播种技术种植玉米具有节本增效、省时省工、增产增收的优势,并指出玉米机械精量播种技术实施过程中的关键环节。     

Effects of glucose and Mg2+ in the neurons damaged by glutamate

AIM and METHODS: To observe the effects of glucose-free and Mg²⁺-free in the extracellular fluid on the changes of [Ca ²⁺]_i in the cerebro-cortical neurons damaged by 1mmol/L glutamate using laser confocal scanning microscope. RESULTS: Both frequency and amplitude of neuronal calcium oscillation induced by glutamate were lowered in glucose-free and Mg²⁺-free buffers. The basic [Ca²⁺]_i concentration was lowered in the former case , but it was elevated in the latter case. CONCLUSION: Mg²⁺-free aggravates [Ca²⁺]_i overload induced by 1mmol/L glutamate ,under certain conditions the glucose-free might resist damage role of glutamate and Mg²⁺-free.     

Effect of ischemia or hypoxia on vascular endothelial growth factor production in rat myocardium and its significance

AIM:To determine the relationship between ischemia, hypoxia and the production of vascular endothelial growth factor in rat myocardium and its basic mechanism. METHODS:(1) 28 Wistar rats were randomly divided into 4 groups: group A, normal control;group B, 1 day's acute myocardial infarction;group C, 3 day's acute myocardial infarction;group D, 7 day's acute myocardial infarction. (2) Rat cardiac myocytes cultured were primarily divided into some groups, hypoxia incubated 24 hours; PMA groups, hypoxia incubated 24 hours with PKC activator (PMA), A 0 ng/mL; B 10 ng/mL; C 100 ng/mL; D 1 000 ng/mL; Chelerythrine groups, hypoxia incubated 24 hours with PKC inhibitor (chelerythrine), A 0 nmol/L; B 10 nmol/L. (3) By computer scanned and quantitated, vascular endothelial growth factor (VEGF) protein was detected with immunohistochemical technique. RESULTS:The longer time of ischemia and hypoxia was, the higher the VEGF production.The relat ionship was found between the time of ischemia or hypoxia and the production of VEGF.The product ion of VEGF protein was further promoted by PMA with different concentrat ion, decreased by chelerythrine.CONCLUSION: Ischemia or hypoxia strongly stimulated the production of VEGF in myocardium, which played an important role in autoprotecting of ischemic or hypoxic myocardium. Hypoxia-induced PKC activation is one kind of basic mechanisms in this course.     

Mechanical insights into the effect of fluctuation in soil moisture on nitrous oxide emissions from paddy soil

Paddy fields are subjected to fluctuating water regimes as a result of the alternate drying and wetting water management, which often incurs a sensitive change in N₂O emissions from paddy soils. However, how the soil moisture regulates the emission of N₂O from paddy soil remains uncertain. In this study, three incubation experiments were designed to study the effects of constant and fluctuating soil moisture on N₂O emission and the sources of N₂O emission from paddy soil. Results showed that the N₂O emission from paddy soil at 100 % WHC (water-holding capacity) was higher than that at 40, 65, 80, 120, and 160 % WHC, indicating that 100 % WHC was the optimum soil moisture content for N₂O emission under the incubation experiment. Small peak of N₂O flux appeared when the soil moisture content from 250 % WHC decreased near to 100 % WHC, lower than that triggered by nitrogen (N) fertilization, which was mainly owing to the low NH₄ ⁺ concentration at this period. Nitrification dominated the emissions of N₂O from paddy soil at 250 % WHC (54.96 %), higher than that of nitrification-coupled denitrification (6.74 %) and denitrification (38.3 %). The contribution of denitrification to N₂O emissions (44.10 %) was equivalent to that of nitrification (44.45 %) in soil at 100 % WHC, which was higher than that of 250 % WHC treatment. In conclusion, the finding suggested that the peak of N₂O in paddy soils during midseason aeration could be attributed to the occurrence of optimum soil moisture under sufficient N availability, favorable for the production and accumulation of N₂O.     

Investigation and genetic mapping of a Glomerella leaf spot resistance locus in apple

Apple Glomerella leaf spot (GLS) is a severe fungal disease that damages apple leaves during the summer in China. Breeding new apple varieties that are resistant to the disease is considered the best way of controlling GLS. Fine mapping and tightly linked marker are critically essential for the preselection of resistant seedlings. In this study, a population of 207 F₁ individuals derived from a cross between ‘Golden Delicious’ and ‘Fuji’ was used to construct a fine simple sequence repeat (SSR)‐based genetic linkage map. The position of R_gls, a locus responsible for resistance to GLS, was identified on apple linkage group (LG) 15 using SSR markers CH05g05 and CH01d08, which was adapted from a published set of 300 SSR markers that were developed using the bulked segregant analysis (BSA) method. These two SSR markers flanked the gene, and its recombination rate was 8.7% and 23.2%, respectively. A total of 276 newly developed SSR markers around the target region and designed from the genome apple assembly contig of LG15 were screened. Only nine of these were determined to be linked to the R_gls locus. Thus, a total of 11 SSR markers were in linkage with R_gls, and mapped at distances ranging from 0.5 to 33.8 cM. The closest marker to the R_gls locus was S0405127, which showed a genetic distance of approximately 0.5 cM. The first mapping of the gene R_gls was constructed, and the locations of the 11 effective primers in the ‘Golden Delicious’ apple genome sequence were anchored. This result facilitates better understanding of the molecular mechanisms underlying the trait of resistance to GLS and could be used in improving the breeding efficiency of GLS‐resistant apple varieties.     

Preliminary study of patients with chronic mountain sickness by GC-TOF-MS based serum metabolomics analysis

AIM: To evaluate specific metabolomics profiles in the serum of patients with chronic mountain sickness (CMS) and to explore the potential metabolic biomarkers in the native Tibetans living on the Qinghai-Tibet Pla-teau.METHODS: A gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) approach as a metabolomics technique was used to evaluate metabolic differences. The native Tibetan CMS patients (n=10) and healthy Tibetan controls (n=10) were enrolled from YuShu in Qinghai province in this study. The serum samples were collected and analyzed by GC-TOF-MS coupled with a series of multivariate statistical analyses such as principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA).RESULTS: The intergroup differences between CMS patients and control subjects have been observed. A list of differential metabolites and several top altered metabolic pathways have been identified. The levels of fumaric acid, an intermediate in the tricarboxylic acid (TCA) cycle, and inosine were highly upregulated in the CMS patients, suggesting a greater effort to hypoxic adaptation in high elevation area. Other differential metabolites, such as methyl phosphate, 2-ketoadipate, lyxose and phytanic acid were also identified. Importantly, the differential metabolites possessed higher area under the ROC curve (AUC) values, indicating an excellent clinical ability for the prediction of CMS. Increased levels of amino acids (isoleucine, glycine, serine, L-cysteine, citrulline and trimethyllysine) were detected in CMS group, yet significantly decreased levels of sulfuric acid, oxamic acid, lyxose and glutamine were also detected in CMS group than those in control group. At the same time, the levels of ribose and glucose-1-phosphate were markedly elevated in CMS group (P<0.05).CONCLUSION: The metabolic activities are significantly altered in the serum of CMS patients. High altitude hypoxia may act on the disturbed glucose metabolism and amino acid metabolism in part of the Tibetan triggered by CMS.     

玉树独一味异型花柱对结实率影响的初步研究

[目的]研究独一味异型花柱对结实率的影响.[方法]通过对独一味异型花柱在花序中不同位置和不同花期中的分布、盛花期异型花柱花部特征参数、异型花柱中花粉活力及柱头活性差异及花粉管在柱头上的萌发、花序不同部位自然结实率方面的研究,探讨独一味异型花柱与结实率的相关性.[结果]独一味L型花柱上花粉的萌发高于S型,L型花柱的位置和结实种子的位置具有一致性.[结论]独一味异型花柱与结实存在一定的相关性.