Twin-Screw Extrusion Processing of Feed Blends Containing Distillers Dried Grains with Solubles (DDGS)

Extrusion trials were conducted with varying levels of distillers dried grains with solubles (DDGS) along with soy flour, corn flour, fish meal, vitamin mix, mineral mix, and net protein content adjusted to 28% using a Wenger TX-52 twin-screw extruder. The properties of extrudates were studied in experiments conducted using a full-factorial design with three levels of DDGS content, two levels of moisture content, and two levels of screw speed. Increasing the DDGS content from 20 to 60% resulted in a 36.7% decrease in the radial expansion, leading to a 159 and 61.4% increase in the unit density and bulk density of the extrudates, respectively. Increasing the DDGS content resulted in a significant increase in the water absorption index (WAI) but a significant decrease in the water solubility index (WSI) of the extrudates. Changing the screw speed and moisture content had no significant effect on the radial expansion ratio but resulted in a significant difference in the bulk density of the extrudates, which may be due to the occurrence of longitudinal expansion. Even though changing the moisture content and screw speed had no significant effect on the WSI of the extrudates, significant differences in the WAI of the extrudates were observed. The ingredient components in the blend and moisture content had an influence on the color changes of the extrudates, as did the biochemical changes occurring inside the barrel during processing. Overall, it was determined that DDGS could be included at a rate of up to 60% using twin-screw extrusion, and that viable pelleted floating feeds can be produced.     

Response of snails and slugs to fragmentation of lowland forests in NW Germany

Habitat fragmentation is a major cause for species loss, but its effect on invertebrates with low active dispersal power, like terrestrial gastropods, has rarely been studied. Such species can not cross a hostile habitat matrix, for which the predictions of island theory, such as positive relations between species richness and patch size, should apply. In order to test this prediction, we studied gastropod species diversity by assessing gastropod assemblage characteristics from 35 sites in 19 fragments of deciduous old-growth forests in the Lower Rhine Embayment, Germany. Assemblages differed between larger (≥700 ha) and smaller forests (<400 ha), those of large forests held a higher percentage of forest species. Although α-diversity was similar between the two forest size classes, small forests often comprised matrix species, resulting in a higher β-diversity. Edge effects on the species richness of matrix species were noticeable up to 250 m into the forest. Hierarchical partitioning revealed that distance to disturbances (external edge, internal edges like roads) explained most assemblage variables, whereas forest size and woodland cover within a 1 km radius from the sites explained only a few assemblage variables. Densities of two forest-associated species, Discus rotundatus and Arion fuscus, decreased with forest size. Yet, forest size was positively correlated with richness of typical forest species and densities of Limax cinereoniger. The latter species seems to need forests of >1,000 ha, i.e., well above the size of most fragments. In conclusion, the prediction is valid only for forest species. The response to fragmentation is species specific and seems to depend on habitat specialization and macroclimatic conditions. Jean-Pierre Maelfait: Deceased.     

Hematology of the Pascagoula map turtle (Graptemys gibbonsi) and the southeast Asian box turtle (Cuora amboinensis)

Turtle populations are decreasing dramatically due to habitat loss and collection for the food and pet market. This study sought to determine hematologic values in two species of turtles to help assess health status of captive and wild populations. Blood samples were collected from 12 individuals of the Pascagoula map turtle (Graptemys gibbonsi) and seven individuals of the southeast Asian box turtle (Cuora amboinensis) from the Savannah River Ecology Laboratory (South Carolina, USA). The hematologic data included hematocrit, total solids, erythrocyte count, leukocyte count, and differential and percentage leukocyte counts. Low hematocrit values and high basophil counts were found in both species. The basophil was the most abundant leukocyte in the Pascagoula map turtle (median = 0.80 x 10(9)/L), whereas in the Southeast Asian box turtle the most abundant leukocyte was the heterophil (median = 2.06 x 10(9)/L).     

Temporal change in fragmentation of continental US forests

Changes in forest ecosystem function and condition arise from changes in forest fragmentation. Previous studies estimated forest fragmentation for the continental United States (US). In this study, new temporal land-cover data from the National Land Cover Database (NLCD) were used to estimate changes in forest fragmentation at multiple scales for the continental US. Early and late dates for the land-cover change data were ca. 1992 and ca. 2001. Forest density was used as a multi-scale index of fragmentation by measuring the proportion of forest in neighborhoods ranging in size from 2.25 to 5314.41 ha. The multi-scale forest density maps were classified using thresholds of 40% (patch), 60% (dominant), and 90% (interior) to analyze temporal change of fragmentation. The loss of dominant and interior forest showed distinct scale effects, whereas loss of patch forest was much less scale-dependent. Dominant forest loss doubled from the smallest to the largest spatial scale, while interior forest loss increased by approximately 80% from the smallest to the second largest spatial scale, then decreased somewhat. At the largest spatial scale, losses of dominant and interior forest were 5 and 10%, respectively, of their ca. 1992 amounts. In contrast, patch forest loss increased by only 25% from the smallest to largest spatial scale. These results indicate that continental US forests were sensitive to forest loss because of their already fragmented state. Forest loss would have had to occur in an unlikely spatial pattern in order to avoid the proportionately greater impact on dominant and interior forest at larger spatial scales.     

Mapping Spatial Patterns with Morphological Image Processing

We use morphological image processing for classifying spatial patterns at the pixel level on binary land-cover maps. Land-cover pattern is classified as ‘perforated,’ ‘edge,’ ‘patch,’ and ‘core’ with higher spatial precision and thematic accuracy compared to a previous approach based on image convolution, while retaining the capability to label these features at the pixel level for any scale of observation. The implementation of morphological image processing is explained and then demonstrated, with comparisons to results from image convolution, for a forest map of the Val Grande National Park in North Italy.     

The Efficacy of a Novel Microbial 6‐Phytase Expressed in Aspergillus oryzae on the Performance and Phosphorus Utilization of Cold‐ and Warm‐Water Fish: Rainbow Trout,Oncorhynchus mykiss,and Nile Tilapia,Oreochromis niloticus

The efficacy and tolerance of a novel microbial 6‐phytase were investigated in rainbow trout, Oncorhynchus mykiss, and Nile tilapia, Oreochromis niloticus. Reference diets were sufficient in available phosphorus (P). The test diet limiting in available P was supplemented with phytase at 500, 1000, or 2000 phytase units/kg feed. The enzyme was effective in increasing total P apparent digestibility coefficient in relation to increasing the dose of phytase in rainbow trout and Nile tilapia. Zinc apparent digestibility improved in relation to phytase supplementation in rainbow trout. P release due to phytase supplementation ranged from 0.06 to 0.18% P/kg feed in rainbow trout and from 0.13 to 0.26% P/kg feed in Nile tilapia. A 58‐d performance trial was conducted to evaluate tolerance of fish to phytase supplementation. Dietary treatments consisted of a basal diet without phytase or supplemented with 2000 and 200,000 phytase units/kg feed. Results indicate that this novel microbial 6‐phytase is well tolerated by fish. Significant improvements for growth as well as feed conversion ratio were observed when the phytase was fed at 2000 phytase units/kg feed. This phytase is proven efficient in releasing P from phytate and could be added when plants are used for fish meal replacement in diets for salmonid and omnivorous fish.     

The complementary role of lentic and lotic habitats for Arctic grayling in a complex stream‐lake network in Arctic Alaska

Lakes can be important to stream dwelling fishes, yet how individuals exploit habitat heterogeneity across complex stream‐lake networks is poorly understood. Furthermore, despite growing awareness that intermittent streams are widely used by fish, studies documenting the use of seasonally accessible lakes remain scarce. We studied Arctic grayling (Thymallus arcticus) in a small seasonally flowing (June–October) stream‐lake network in Alaska using PIT telemetry. Overall, 70% of fish visited two lakes, 8% used a single lake, and 22% used only stream reaches. We identified five distinct behavioural patterns that differed in dominant macrohabitat used (deep lake, shallow lake or stream reaches), entry day into the network and mobility. Some juvenile fish spent the entire summer in a shallow seasonally frozen lake (average 71 days), whereas others demonstrated prospecting behaviour and only entered the stream channel briefly in September. Another group included adult and juvenile fish that were highly mobile, moving up to 27 km while in the 3‐km stream‐lake network, and used stream reaches extensively (average 59 days). Lentic and lotic habitats served differing roles for individuals, some fish occupied stream reaches as summer foraging habitat, and other individuals used them as migration corridors to access lakes. Our study emphasises the importance of considering stream‐lake connectivity in stream fish assessments, even to shallow seasonally frozen habitats not widely recognised as important. Furthermore, we demonstrate that individuals may use temporary aquatic habitats in complex and changing ways across ontogeny that are not captured by typical classifications of fish movement behaviour.     

Landscape hierarchies influence riparian ground-flora communities in Wisconsin, USA

We examined the distribution of ground-flora species (herbaceous and woody species <1 m tall) across riparian areas of northeastern Wisconsin in an effort to understand how hierarchical landscape properties, such as the physiographic system (ground moraine and outwash plain), valley system (constrained and unconstrained), and valley floor landforms influence distribution patterns of ground-flora species and functional plant guilds across riparian areas of small streams and rivers in a glacial landscape. A total of 162 species were recorded on 417 (1 m²) plots stratified by four different valley types that reflect the dominant physiographic system and valley system (constrained ground moraine, constrained outwash plain, unconstrained ground moraine, unconstrained outwash plain) and transverse geomorphic structure (valley floor landforms including floodplains, terraces, slopes, and adjacent uplands). Although distribution patterns of individual ground-flora species are highly variable among the four valley types, canonical correspondence analyses (CCA) of individual valley types indicate that ground-flora vegetation is related strongly to hierarchical landscape properties, including valley type and the transverse geomorphic structure of the stream valley. Vegetation ultimately reflects the influence of hydrogeomorphic processes that shape valley floor landforms. Constrained valley types tend to be characterized by diverse floodplain ground-flora communities dominated by graminoids and pteridophytes, while the terraces and slope plant communities are comprised of facultative upland and obligate upland perennial forb and woody species whose distribution appears to be a function of topographic features, such as aspect. However, ground-flora communities of unconstrained valley types appear to be responding not only to changes associated with the transverse geomorphic structure of the riparian ecotone, but also to more localized changes in environmental conditions associated with flooding and hydrologic regime. As a result, floodplain, terrace, and slope ground-flora communities tend to be diverse, dominated by a mixture of obligate wetland, facultative wetland, and facultative species. Thus, variation in riparian plant community characteristics can be explained using a nested, hierarchical landscape framework to organize and group different riparian settings based on the underlying geomorphic processes shaping stream valleys. Based on these results, we suggest that riparian management zones (RMZs) designed to maintain riparian function should be variable in width rather than fixed, encompassing variation in valley floor landforms and valley walls, regardless of the physiographic system.     

Shoot water relations of mature black spruce families displaying a genotype x environment interaction in growth rate. I. Family and site effects over three growing seasons

Pressure-volume curves were determined for black spruce (Picea mariana (Mill.) BSP) trees from four full-sib families. During the first two years, trees were measured from a plantation on a dry site. In the third year, trees were sampled from the dry site and a wet site. Diurnal measurements of shoot water potential allowed in situ shoot turgor to be estimated in addition to standard water relations traits. Over all years, Female 59 progeny displayed lower osmotic potentials at saturation (Psi(sat)) than Female 63 progeny. Genetic differences in Psi(sat) were similar on both the dry and wet sites. Modulus of elasticity (epsilon) was greater for Female 59 progeny than for Female 63 progeny, producing a compensatory effect resulting in no genetic or site differences in osmotic potential at turgor loss point (Psi(tlp)) or relative water content at turgor loss point (RWC(tlp)). Mean and predawn shoot turgor pressures (P(x) and P(pd)) were higher for Female 59 progeny than for Female 63 progeny and higher at the wet site than the dry site. Genotype x environment trends were observed; compared to Female 63 progeny, Female 59 progeny displayed 9.8 and 5.1% higher P(pd) on the dry and wet sites, respectively, and 3.4 and 9.8% greater P(pd) values in wet and dry years, respectively. Tree volume growth showed no relationship to Psi(tlp) or RWC(tlp), but was correlated with Psi(sat) and P(x); however, the strongest correlation was with P(pd) (r = 0.90).