荒川库蠓人工繁殖的研究

实验室人工模拟条件下成功地进行荒川库蠓的饲养和繁殖。荒川库蠓吸鸡血的饱血时间为10min，饱血率为88.46%，饱血库蠓饲养3d后产卵，产卵率为69.56%。产卵后第2天开始孵化，一般在24h内孵化结束，孵化率为76.75%。幼虫先后蜕皮3次，共分4龄幼虫，1-4龄幼虫的体长分别为：0.3-1.12mm,1.12-3.02mm,3.02-4.55mm和4.55-5.90mm，其幼虫期分别为7、4、4和5d。4龄幼虫化蛹2-3d后即羽化为成蠓，羽化为81.82%,不同温度条件对卵到成蠓的发育影响较大，26℃是荒川库蠓发育相对适宜的温度条件。     

饲料钙测定中原子吸收法和高锰酸钾滴定法的比较

在国家标准中，饲料中钙的测定方法是高锰酸钾滴定法。其方法原理是：将试样中有机物破坏，钙变成溶于水的离子，用草酸铵定量沉淀，用高锰酸钾法间接测定钙含量。此方法前处理较繁琐，前后需三天时间，检测周期较长。对于大批量的样品检测的速度带来一定的影响。原子吸收法测定比高锰酸钾滴定法更简便和直接，并且更精确。其方法原理为：样品经湿法消化，分解有机质后，将钙元素溶解在稀酸中，将溶液直接吸入空气－乙炔火焰中原子化，并在光路中测定钙原子对特定波长谱线的吸收。1 仪器和试剂1.1 仪器原子吸收分光光度计(美国瓦里安公司，…     

低温胁迫对草地早熟禾抗性生理生化指标的影响

研究低温胁迫对草地早熟禾生理生化指标的影响。结果表明,在5℃下,超氧化物岐化酶(SOD)和过氧化氢酶(CAT)的活性随着胁迫时间的延长而增加,当4h时出现最大值,之后活性逐渐下降。当温度为0℃与-6℃时,两种酶的活性随着胁迫时间的延长而急剧下降,接近于零,温度越低,下降越快。叶绿素和类胡萝卜素含量随着肋迫时间的延长而下降,温度越低下降越快。5℃对脯氨酸含量无明显影响,在0℃与-6℃时,随着时间的延长,含量则明显增加。5℃和0℃低温胁迫对质膜相对透性无明显影响,-6℃时,质膜相对透性明显增大。供试指标对低温胁迫敏感性的顺序是:超氧化物岐化酶、过氧化氢酶>类胡萝卜素>脯氨酸>叶绿素>质膜相对透性。     

MAGNETIC RESONANCE IMAGING OF THE CANINE OPTIC NERVE

We describe the magnetic resonance (MR) imaging aspects of normal canine optic nerve, the diameter of the optic nerve as measured on MR images, and optimal MR sequences for the evaluation of the optic nerve using a 0.2 T MR unit. Three millimeter contiguous slides of the normal canine orbital region were acquired in transverse and dorsal oblique planes using a variety of tissue weighting sequences. It was apparent that detailed anatomic assessment of the optic nerve can be performed with low‐field MR imaging, but none of the sequences provided unequivocal superior image quality of the optic nerve. The mean diameter of the optic nerve sheath complex was 3.7 mm and of the optic nerve 1.7 mm. The intraorbital and intracanalicular parts of the optic nerve are consistently visible and differentiation between the optic nerve and optic nerve sheath complex is possible using low‐field MR systems.     

新疆兵团昭苏垦区家畜包虫病防控情况调查

为了解新疆昭苏垦区包虫病防控工作近况,对新疆生产建设兵团农四师76团和77团饲养的家犬和家畜进行包虫病调查。结果表明,两个团场分别饲养犬1 004条和620条,养殖家畜58 253头(只)和64 848头(只);犬感染细粒棘球绦虫感染率为9.76%(98/1 004)和15.98%(81/507);77团家畜感染包虫病感染率26.45%(338/1 278)。     

裂区设计下分析影响欧洲菊苣产量的因素

在裂区设计下,分析影响欧洲菊苣（Cichorium intybus cv.Europ）产量的7个因素,结果表明：播种季节对株高、产量有显著性影响;播种方式对株高、茎粗、密度、盖度、产量5个指标均有显著影响。进一步通过因子分析得出欧洲菊苣产量最佳组合为撒播、密度在50～59株·m^-2、盖度为94%～100%。     

A retrospective analysis of radiation therapy for the treatment of feline vaccine‐associated sarcoma*

We retrospectively evaluated predictive prognostic factors in 73 cats with vaccine‐associated sarcoma given postsurgical curative (n = 46, most with clean margins) or coarse fractionated radiotherapy (n = 27, most with either macroscopic disease or dirty margins). The former animals displayed a median survival of 43 months and a median progression free interval (PFI) of 37 months, the latter reached a median survival of 24 months and a median PFI of 10 months. In cats undergoing coarse fractionated therapy, factors predictive of a better outcome included lack of visible mass (n = 10) as opposed to macroscopic disease (n = 17, survival: 30 versus 7 months, P = 0.025; PFI: 20 versus 4 months, P = 0.01), adjuvant chemotherapy for gross disease (n = 5/17, survival: 29 versus 5 months, P = 0.04) and a smaller number of surgeries preceding radiation therapy (coeff = 0.41, P = 0.03). The Ki67 index was not predictive for survival. We concluded that postsurgical curative and coarse fractionated radiotherapy are effective legitimate options for managing vaccine‐associated sarcomas.     

Suspected venous air embolism in three horses

Venous air embolism was suspected in 3 hospitalised horses on the basis of an inadvertently open jugular venous line. Subsequently the 3 horses developed clinical signs including cardiovascular collapse, cardiac dysrhythmia, pulmonary oedema, behavioural abnormalities and neurological signs consistent with central nervous system injury. Treatment included intranasal oxygen therapy, i.v. fluid administration and anti‐inflammatory therapy. Specific therapy aimed at treating air embolism in man is reviewed, with pertinent therapy potentially applicable to horses emphasised.     

The coefficient of dominance is not (always) estimable with biallelic markers

The genetic relationship among individuals at one locus is characterized by nine coefficients of identity. The coefficients of inbreeding, coancestry and dominance (or fraternity) are just linear functions of them. Here, it is shown how they can be estimated using biallelic and triallelic markers using the method of moments, and comparisons are made with other methods based on molecular coancestry or molecular covariance. It is concluded that in the general case of dominance and inbreeding with biallelic markers, only the coefficients of inbreeding and coancestry can be estimated, but neither the single coefficients of identity nor the coefficient of dominance can be estimated. More than two alleles are required for a full estimation as illustrated with the triallelic situation.