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111.
Cooked rice of ‘Sasanishiki’ is soft and not as sticky as those of Japanese leading cultivars ‘Koshihikari’ and ‘Hitomebore’. As a method for efficient selection of a breeding line having a good eating quality like that of ‘Sasanishiki’, the use of physical properties of cooked rice and cooking quality was examined. There were differences of physical properties of the surface layer, starch-iodine blue value per solid substance weight in cooking water and volume expansion of cooked rice between ‘Sasanishiki’ and ‘Hitomebore’, these properties being considered to be usable for the selection of breeding lines. Using these traits as selection targets, one line, named ‘Tohoku 194’, which has eating quality highly similar to that of ‘Sasanishiki’ and cold tolerance derived from ‘Hitomebore’, was selected from progeny of a cross between ‘Sasanishiki’ and ‘Hitomebore’. An application for registration as a new variety has been submitted for ‘Tohoku 194’ under the Japanese Plant Variety Protection Act, and is expected to become a recommended cultivar in Miyagi Prefecture. ‘Tohoku 194’ may fulfill various demands of consumers and companies in the food industry. 相似文献
112.
To overcome the lability of vitamin C (L-ascorbic acid or L-AsA), an essential nutrient of shrimp diets, the efficacy of Mg-L-ascorbyl-2-phosphate (MAP), a relatively stable derivative of L-AsA, was examined.
Groups of juvenile Penaeus japonicus were fed five different test diets containing different amounts of MAP for 100 days, to determine if MAP could be utilized by shrimp. Normal growth was observed on test groups fed diets containing 1,078, 430 and 215 mg MAP per kg, whereas mass mortality occurred in populations fed diets containing 43 and 0 mg MAP per kg. The dead shrimp found in the latter two treatments exhibited blackened lesions underneath the exoskeleton. The symptom looked similar to what was reported in previous papers as L-AsA deficiency syndrome. The feeding experiment suggests that MAP can be utilized by P.japonicus as a suitable replacement for L-AsA, and a supplement of 215 to 430 mg in 1 kg is sufficient to prevent clinical signs of vitamin C deficiency.
Repeated measurements of remaining L-AsA and MAP, made after diet processing and storage, demonstrated that MAP was significantly more stable than L-AsA and was less prone to leaching in sea water. 相似文献
Groups of juvenile Penaeus japonicus were fed five different test diets containing different amounts of MAP for 100 days, to determine if MAP could be utilized by shrimp. Normal growth was observed on test groups fed diets containing 1,078, 430 and 215 mg MAP per kg, whereas mass mortality occurred in populations fed diets containing 43 and 0 mg MAP per kg. The dead shrimp found in the latter two treatments exhibited blackened lesions underneath the exoskeleton. The symptom looked similar to what was reported in previous papers as L-AsA deficiency syndrome. The feeding experiment suggests that MAP can be utilized by P.japonicus as a suitable replacement for L-AsA, and a supplement of 215 to 430 mg in 1 kg is sufficient to prevent clinical signs of vitamin C deficiency.
Repeated measurements of remaining L-AsA and MAP, made after diet processing and storage, demonstrated that MAP was significantly more stable than L-AsA and was less prone to leaching in sea water. 相似文献
113.
The ATPase assay system on pH stat apparatus was developed. For the ATPase activity measurement, hydrogen ion (H+) concentration delivered from inorganic phosphate (Pi) as a hydrolysis production of ATP was estimated by titrating with
20 mM NaOH solution instead of colorimetric measurement of Pi. Inclusion of 0.5 M KCl in the ATP stock solution and 2 mM Tris-maleate
(pH 7.0) buffer in the reaction medium reduced the extent of pH change upon addition of ATP to initiate the ATPase reaction.
The amount of H+ liberated from Pi was strongly affected by the set pH for the ATPase assay with a promotion at alkaline pH. Thus, it was
required to estimate the coefficient to convert H+ to a Pi concentration at a specific pH. The specified coefficient at pH 7.0 was 1.248. ATPase assay on pH stat allowed us
to follow the ATP hydrolysis by myofibrils continuously showing a curvature profile at low salt medium (≤0.2 M KCl) or a linear
profile at high salt (≥0.3 M KCl). 相似文献
114.
Muhetaerjiang MUSHA Shuichi HIRAI Munekazu NAITO Hayato TERAYAMA Ning QU Naoyuki HATAYAMA Masahiro ITOH 《The Journal of reproduction and development》2013,59(2):139-144
Experimental autoimmune orchitis (EAO) is a model of immunologic male infertility and
pathologically characterized by lymphocytic inflammation, which causes breakdown of the
testicular immune privilege with spermatogenic disturbance. Generally, murine EAO is
induced by immunization with testicular homogenate (TH) from the testes of donor mice +
complete Freund''s adjuvant (CFA) + Bordetella pertussigens (BP), and it
has been considered that treatment with these two adjuvants is required to enhance the
immune response against testicular antigens. However, there remains a possibility that CFA
and BP may affect autoimmune responses against the testicular antigens without TH. In the
present study, we examined this possibility using real-time RT-PCR, Western blotting and
immunohistochemical staining. The results demonstrated that immunization with TH in
combination with CFA and BP evoked more severe EAO than that with only TH. Real-time
RT-PCR analyses revealed that Fas mRNA expression in TH+CFA+BP-induced EAO was
significantly higher than that in TH-induced EAO. Interestingly, IL-6 mRNA expression
dramatically increased in TH+CFA+BP-induced EAO; however, no apparent change in IL-6 mRNA
expression occurred in TH-induced EAO. It was also noted that treatment with CFA and BP
alone augmented autoimmune reactions against some testicular autoantigens. These results
indicates that these adjuvants are helpful in evoking severe EAO, and treatment with the
adjuvants alone can evoke autoimmune reactions against some testicular autoantigens
despite the use of no TH. 相似文献
115.
116.
Toshihiro Nade Yuya Kataoka Kazuhiro Hoshi Ikuo Maeda Kunihiko Saito 《Animal Science Journal》2014,85(3):247-253
This study investigated an objective method for estimating beef marbling using ultrasonic images of the Iliocostalis muscle and the Lomgissimus muscle area sections. Thirty‐one Japanese Black cattle steers were used in this study. The end of the left side shoulder blade bone was scanned using an ultrasonic device. Ultrasonic images were captured of the Longissimus muscle area and that around the Iliocostalis muscle area. Twenty items were measured in the two images using computer image analysis software. The level of beef marbling was measured according to the Beef Marbling Standard (BMS) for carcass grading, and the percentage of ether‐extractable fat content in the Longissimus muscle (EE). The difference in the gray level between the Iliocostalis muscle and intermuscular fat (X10) was used to estimate the BMS and the EE, which were highly correlated (r2 = 67.72% and 61.30%). An equation was developed using four parameters from the two ultrasonic images, which could estimate the BMS (r2 = 85.88%). This equation could also estimate the EE (r2 = 68.98%). The equations used to estimate beef marbling were based on one to four parameters that included X10. Thus, ultrasonic images of the Iliocostalis muscle area section are important for estimating beef marbling. 相似文献